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Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T

AIM: To demonstrate the design, fabrication and testing of a portable, label-free biosensor for ultrasensitive detection of the cardiac Troponin-T (cTnT) from patient blood. MATERIALS & METHODS: The biosensor is comprised of a nanoporous membrane integrated on to a microelectrode sensor platform...

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Autores principales: Shanmugam, Nandhinee Radha, Selvam, Anjan Panneer, Barrett, Thomas W, Kazmierczak, Steven C, Rana, Milin Nilesh, Prasad, Shalini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137999/
https://www.ncbi.nlm.nih.gov/pubmed/28031897
http://dx.doi.org/10.4155/fso.15.22
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author Shanmugam, Nandhinee Radha
Selvam, Anjan Panneer
Barrett, Thomas W
Kazmierczak, Steven C
Rana, Milin Nilesh
Prasad, Shalini
author_facet Shanmugam, Nandhinee Radha
Selvam, Anjan Panneer
Barrett, Thomas W
Kazmierczak, Steven C
Rana, Milin Nilesh
Prasad, Shalini
author_sort Shanmugam, Nandhinee Radha
collection PubMed
description AIM: To demonstrate the design, fabrication and testing of a portable, label-free biosensor for ultrasensitive detection of the cardiac Troponin-T (cTnT) from patient blood. MATERIALS & METHODS: The biosensor is comprised of a nanoporous membrane integrated on to a microelectrode sensor platform for nanoconfinement effects. Charge perturbations due to antigen binding are recorded as impedance changes using electrochemical impedance spectroscopy. RESULTS: The measured impedance change is used to quantitatively determine the cTnT concentration from the tested sample. We were successful in detecting and quantifying cardiac Troponin-T from a 40-patient cohort. The limit of detection was 0.01 pg/ml. CONCLUSION: This novel technology has promising preliminary results for rapid and sensitive detection of cTnT.
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spelling pubmed-51379992016-12-28 Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T Shanmugam, Nandhinee Radha Selvam, Anjan Panneer Barrett, Thomas W Kazmierczak, Steven C Rana, Milin Nilesh Prasad, Shalini Future Sci OA Preliminary Communication AIM: To demonstrate the design, fabrication and testing of a portable, label-free biosensor for ultrasensitive detection of the cardiac Troponin-T (cTnT) from patient blood. MATERIALS & METHODS: The biosensor is comprised of a nanoporous membrane integrated on to a microelectrode sensor platform for nanoconfinement effects. Charge perturbations due to antigen binding are recorded as impedance changes using electrochemical impedance spectroscopy. RESULTS: The measured impedance change is used to quantitatively determine the cTnT concentration from the tested sample. We were successful in detecting and quantifying cardiac Troponin-T from a 40-patient cohort. The limit of detection was 0.01 pg/ml. CONCLUSION: This novel technology has promising preliminary results for rapid and sensitive detection of cTnT. Future Science Ltd 2015-11-01 /pmc/articles/PMC5137999/ /pubmed/28031897 http://dx.doi.org/10.4155/fso.15.22 Text en © S Prasad et al. This work is licensed under a Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/)
spellingShingle Preliminary Communication
Shanmugam, Nandhinee Radha
Selvam, Anjan Panneer
Barrett, Thomas W
Kazmierczak, Steven C
Rana, Milin Nilesh
Prasad, Shalini
Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T
title Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T
title_full Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T
title_fullStr Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T
title_full_unstemmed Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T
title_short Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T
title_sort portable nanoporous electrical biosensor for ultrasensitive detection of troponin-t
topic Preliminary Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137999/
https://www.ncbi.nlm.nih.gov/pubmed/28031897
http://dx.doi.org/10.4155/fso.15.22
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