Cargando…
Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T
AIM: To demonstrate the design, fabrication and testing of a portable, label-free biosensor for ultrasensitive detection of the cardiac Troponin-T (cTnT) from patient blood. MATERIALS & METHODS: The biosensor is comprised of a nanoporous membrane integrated on to a microelectrode sensor platform...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Future Science Ltd
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137999/ https://www.ncbi.nlm.nih.gov/pubmed/28031897 http://dx.doi.org/10.4155/fso.15.22 |
_version_ | 1782471997467394048 |
---|---|
author | Shanmugam, Nandhinee Radha Selvam, Anjan Panneer Barrett, Thomas W Kazmierczak, Steven C Rana, Milin Nilesh Prasad, Shalini |
author_facet | Shanmugam, Nandhinee Radha Selvam, Anjan Panneer Barrett, Thomas W Kazmierczak, Steven C Rana, Milin Nilesh Prasad, Shalini |
author_sort | Shanmugam, Nandhinee Radha |
collection | PubMed |
description | AIM: To demonstrate the design, fabrication and testing of a portable, label-free biosensor for ultrasensitive detection of the cardiac Troponin-T (cTnT) from patient blood. MATERIALS & METHODS: The biosensor is comprised of a nanoporous membrane integrated on to a microelectrode sensor platform for nanoconfinement effects. Charge perturbations due to antigen binding are recorded as impedance changes using electrochemical impedance spectroscopy. RESULTS: The measured impedance change is used to quantitatively determine the cTnT concentration from the tested sample. We were successful in detecting and quantifying cardiac Troponin-T from a 40-patient cohort. The limit of detection was 0.01 pg/ml. CONCLUSION: This novel technology has promising preliminary results for rapid and sensitive detection of cTnT. |
format | Online Article Text |
id | pubmed-5137999 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Future Science Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-51379992016-12-28 Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T Shanmugam, Nandhinee Radha Selvam, Anjan Panneer Barrett, Thomas W Kazmierczak, Steven C Rana, Milin Nilesh Prasad, Shalini Future Sci OA Preliminary Communication AIM: To demonstrate the design, fabrication and testing of a portable, label-free biosensor for ultrasensitive detection of the cardiac Troponin-T (cTnT) from patient blood. MATERIALS & METHODS: The biosensor is comprised of a nanoporous membrane integrated on to a microelectrode sensor platform for nanoconfinement effects. Charge perturbations due to antigen binding are recorded as impedance changes using electrochemical impedance spectroscopy. RESULTS: The measured impedance change is used to quantitatively determine the cTnT concentration from the tested sample. We were successful in detecting and quantifying cardiac Troponin-T from a 40-patient cohort. The limit of detection was 0.01 pg/ml. CONCLUSION: This novel technology has promising preliminary results for rapid and sensitive detection of cTnT. Future Science Ltd 2015-11-01 /pmc/articles/PMC5137999/ /pubmed/28031897 http://dx.doi.org/10.4155/fso.15.22 Text en © S Prasad et al. This work is licensed under a Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/) |
spellingShingle | Preliminary Communication Shanmugam, Nandhinee Radha Selvam, Anjan Panneer Barrett, Thomas W Kazmierczak, Steven C Rana, Milin Nilesh Prasad, Shalini Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T |
title | Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T |
title_full | Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T |
title_fullStr | Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T |
title_full_unstemmed | Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T |
title_short | Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T |
title_sort | portable nanoporous electrical biosensor for ultrasensitive detection of troponin-t |
topic | Preliminary Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137999/ https://www.ncbi.nlm.nih.gov/pubmed/28031897 http://dx.doi.org/10.4155/fso.15.22 |
work_keys_str_mv | AT shanmugamnandhineeradha portablenanoporouselectricalbiosensorforultrasensitivedetectionoftroponint AT selvamanjanpanneer portablenanoporouselectricalbiosensorforultrasensitivedetectionoftroponint AT barrettthomasw portablenanoporouselectricalbiosensorforultrasensitivedetectionoftroponint AT kazmierczakstevenc portablenanoporouselectricalbiosensorforultrasensitivedetectionoftroponint AT ranamilinnilesh portablenanoporouselectricalbiosensorforultrasensitivedetectionoftroponint AT prasadshalini portablenanoporouselectricalbiosensorforultrasensitivedetectionoftroponint |