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Methodology for High-Throughput Field Phenotyping of Canopy Temperature Using Airborne Thermography

Lower canopy temperature (CT), resulting from increased stomatal conductance, has been associated with increased yield in wheat. Historically, CT has been measured with hand-held infrared thermometers. Using the hand-held CT method on large field trials is problematic, mostly because measurements ar...

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Autores principales: Deery, David M., Rebetzke, Greg J., Jimenez-Berni, Jose A., James, Richard A., Condon, Anthony G., Bovill, William D., Hutchinson, Paul, Scarrow, Jamie, Davy, Robert, Furbank, Robert T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5138222/
https://www.ncbi.nlm.nih.gov/pubmed/27999580
http://dx.doi.org/10.3389/fpls.2016.01808
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author Deery, David M.
Rebetzke, Greg J.
Jimenez-Berni, Jose A.
James, Richard A.
Condon, Anthony G.
Bovill, William D.
Hutchinson, Paul
Scarrow, Jamie
Davy, Robert
Furbank, Robert T.
author_facet Deery, David M.
Rebetzke, Greg J.
Jimenez-Berni, Jose A.
James, Richard A.
Condon, Anthony G.
Bovill, William D.
Hutchinson, Paul
Scarrow, Jamie
Davy, Robert
Furbank, Robert T.
author_sort Deery, David M.
collection PubMed
description Lower canopy temperature (CT), resulting from increased stomatal conductance, has been associated with increased yield in wheat. Historically, CT has been measured with hand-held infrared thermometers. Using the hand-held CT method on large field trials is problematic, mostly because measurements are confounded by temporal weather changes during the time required to measure all plots. The hand-held CT method is laborious and yet the resulting heritability low, thereby reducing confidence in selection in large scale breeding endeavors. We have developed a reliable and scalable crop phenotyping method for assessing CT in large field experiments. The method involves airborne thermography from a manned helicopter using a radiometrically-calibrated thermal camera. Thermal image data is acquired from large experiments in the order of seconds, thereby enabling simultaneous measurement of CT on potentially 1000s of plots. Effects of temporal weather variation when phenotyping large experiments using hand-held infrared thermometers are therefore reduced. The method is designed for cost-effective and large-scale use by the non-technical user and includes custom-developed software for data processing to obtain CT data on a single-plot basis for analysis. Broad-sense heritability was routinely >0.50, and as high as 0.79, for airborne thermography CT measured near anthesis on a wheat experiment comprising 768 plots of size 2 × 6 m. Image analysis based on the frequency distribution of temperature pixels to remove the possible influence of background soil did not improve broad-sense heritability. Total image acquisition and processing time was ca. 25 min and required only one person (excluding the helicopter pilot). The results indicate the potential to phenotype CT on large populations in genetics studies or for selection within a plant breeding program.
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spelling pubmed-51382222016-12-20 Methodology for High-Throughput Field Phenotyping of Canopy Temperature Using Airborne Thermography Deery, David M. Rebetzke, Greg J. Jimenez-Berni, Jose A. James, Richard A. Condon, Anthony G. Bovill, William D. Hutchinson, Paul Scarrow, Jamie Davy, Robert Furbank, Robert T. Front Plant Sci Plant Science Lower canopy temperature (CT), resulting from increased stomatal conductance, has been associated with increased yield in wheat. Historically, CT has been measured with hand-held infrared thermometers. Using the hand-held CT method on large field trials is problematic, mostly because measurements are confounded by temporal weather changes during the time required to measure all plots. The hand-held CT method is laborious and yet the resulting heritability low, thereby reducing confidence in selection in large scale breeding endeavors. We have developed a reliable and scalable crop phenotyping method for assessing CT in large field experiments. The method involves airborne thermography from a manned helicopter using a radiometrically-calibrated thermal camera. Thermal image data is acquired from large experiments in the order of seconds, thereby enabling simultaneous measurement of CT on potentially 1000s of plots. Effects of temporal weather variation when phenotyping large experiments using hand-held infrared thermometers are therefore reduced. The method is designed for cost-effective and large-scale use by the non-technical user and includes custom-developed software for data processing to obtain CT data on a single-plot basis for analysis. Broad-sense heritability was routinely >0.50, and as high as 0.79, for airborne thermography CT measured near anthesis on a wheat experiment comprising 768 plots of size 2 × 6 m. Image analysis based on the frequency distribution of temperature pixels to remove the possible influence of background soil did not improve broad-sense heritability. Total image acquisition and processing time was ca. 25 min and required only one person (excluding the helicopter pilot). The results indicate the potential to phenotype CT on large populations in genetics studies or for selection within a plant breeding program. Frontiers Media S.A. 2016-12-06 /pmc/articles/PMC5138222/ /pubmed/27999580 http://dx.doi.org/10.3389/fpls.2016.01808 Text en Copyright © 2016 Deery, Rebetzke, Jimenez-Berni, James, Condon, Bovill, Hutchinson, Scarrow, Davy and Furbank. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Deery, David M.
Rebetzke, Greg J.
Jimenez-Berni, Jose A.
James, Richard A.
Condon, Anthony G.
Bovill, William D.
Hutchinson, Paul
Scarrow, Jamie
Davy, Robert
Furbank, Robert T.
Methodology for High-Throughput Field Phenotyping of Canopy Temperature Using Airborne Thermography
title Methodology for High-Throughput Field Phenotyping of Canopy Temperature Using Airborne Thermography
title_full Methodology for High-Throughput Field Phenotyping of Canopy Temperature Using Airborne Thermography
title_fullStr Methodology for High-Throughput Field Phenotyping of Canopy Temperature Using Airborne Thermography
title_full_unstemmed Methodology for High-Throughput Field Phenotyping of Canopy Temperature Using Airborne Thermography
title_short Methodology for High-Throughput Field Phenotyping of Canopy Temperature Using Airborne Thermography
title_sort methodology for high-throughput field phenotyping of canopy temperature using airborne thermography
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5138222/
https://www.ncbi.nlm.nih.gov/pubmed/27999580
http://dx.doi.org/10.3389/fpls.2016.01808
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