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In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia
Graphene, graphene-based nanomaterials (GBNs), and carbon nanotubes (CNTs) are being investigated as potential substrates for the growth of neural cells. However, in most in vitro studies, the cells were seeded on these materials coated with various proteins implying that the observed effects on the...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5138223/ https://www.ncbi.nlm.nih.gov/pubmed/27999773 http://dx.doi.org/10.3389/fbioe.2016.00094 |
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author | Defteralı, Çağla Verdejo, Raquel Majeed, Shahid Boschetti-de-Fierro, Adriana Méndez-Gómez, Héctor R. Díaz-Guerra, Eva Fierro, Daniel Buhr, Kristian Abetz, Clarissa Martínez-Murillo, Ricardo Vuluga, Daniela Alexandre, Michaël Thomassin, Jean-Michel Detrembleur, Christophe Jérôme, Christine Abetz, Volker López-Manchado, Miguel Ángel Vicario-Abejón, Carlos |
author_facet | Defteralı, Çağla Verdejo, Raquel Majeed, Shahid Boschetti-de-Fierro, Adriana Méndez-Gómez, Héctor R. Díaz-Guerra, Eva Fierro, Daniel Buhr, Kristian Abetz, Clarissa Martínez-Murillo, Ricardo Vuluga, Daniela Alexandre, Michaël Thomassin, Jean-Michel Detrembleur, Christophe Jérôme, Christine Abetz, Volker López-Manchado, Miguel Ángel Vicario-Abejón, Carlos |
author_sort | Defteralı, Çağla |
collection | PubMed |
description | Graphene, graphene-based nanomaterials (GBNs), and carbon nanotubes (CNTs) are being investigated as potential substrates for the growth of neural cells. However, in most in vitro studies, the cells were seeded on these materials coated with various proteins implying that the observed effects on the cells could not solely be attributed to the GBN and CNT properties. Here, we studied the biocompatibility of uncoated thermally reduced graphene (TRG) and poly(vinylidene fluoride) (PVDF) membranes loaded with multi-walled CNTs (MWCNTs) using neural stem cells isolated from the adult mouse olfactory bulb (termed aOBSCs). When aOBSCs were induced to differentiate on coverslips treated with TRG or control materials (polyethyleneimine-PEI and polyornithine plus fibronectin-PLO/F) in a serum-free medium, neurons, astrocytes, and oligodendrocytes were generated in all conditions, indicating that TRG permits the multi-lineage differentiation of aOBSCs. However, the total number of cells was reduced on both PEI and TRG. In a serum-containing medium, aOBSC-derived neurons and oligodendrocytes grown on TRG were more numerous than in controls; the neurons developed synaptic boutons and oligodendrocytes were more branched. In contrast, neurons growing on PVDF membranes had reduced neurite branching, and on MWCNTs-loaded membranes oligodendrocytes were lower in numbers than in controls. Overall, these findings indicate that uncoated TRG may be biocompatible with the generation, differentiation, and maturation of aOBSC-derived neurons and glial cells, implying a potential use for TRG to study functional neuronal networks. |
format | Online Article Text |
id | pubmed-5138223 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-51382232016-12-20 In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia Defteralı, Çağla Verdejo, Raquel Majeed, Shahid Boschetti-de-Fierro, Adriana Méndez-Gómez, Héctor R. Díaz-Guerra, Eva Fierro, Daniel Buhr, Kristian Abetz, Clarissa Martínez-Murillo, Ricardo Vuluga, Daniela Alexandre, Michaël Thomassin, Jean-Michel Detrembleur, Christophe Jérôme, Christine Abetz, Volker López-Manchado, Miguel Ángel Vicario-Abejón, Carlos Front Bioeng Biotechnol Bioengineering and Biotechnology Graphene, graphene-based nanomaterials (GBNs), and carbon nanotubes (CNTs) are being investigated as potential substrates for the growth of neural cells. However, in most in vitro studies, the cells were seeded on these materials coated with various proteins implying that the observed effects on the cells could not solely be attributed to the GBN and CNT properties. Here, we studied the biocompatibility of uncoated thermally reduced graphene (TRG) and poly(vinylidene fluoride) (PVDF) membranes loaded with multi-walled CNTs (MWCNTs) using neural stem cells isolated from the adult mouse olfactory bulb (termed aOBSCs). When aOBSCs were induced to differentiate on coverslips treated with TRG or control materials (polyethyleneimine-PEI and polyornithine plus fibronectin-PLO/F) in a serum-free medium, neurons, astrocytes, and oligodendrocytes were generated in all conditions, indicating that TRG permits the multi-lineage differentiation of aOBSCs. However, the total number of cells was reduced on both PEI and TRG. In a serum-containing medium, aOBSC-derived neurons and oligodendrocytes grown on TRG were more numerous than in controls; the neurons developed synaptic boutons and oligodendrocytes were more branched. In contrast, neurons growing on PVDF membranes had reduced neurite branching, and on MWCNTs-loaded membranes oligodendrocytes were lower in numbers than in controls. Overall, these findings indicate that uncoated TRG may be biocompatible with the generation, differentiation, and maturation of aOBSC-derived neurons and glial cells, implying a potential use for TRG to study functional neuronal networks. Frontiers Media S.A. 2016-12-06 /pmc/articles/PMC5138223/ /pubmed/27999773 http://dx.doi.org/10.3389/fbioe.2016.00094 Text en Copyright © 2016 Defteralı, Verdejo, Majeed, Boschetti-de-Fierro, Méndez-Gómez, Díaz-Guerra, Fierro, Buhr, Abetz, Martínez-Murillo, Vuluga, Alexandre, Thomassin, Detrembleur, Jérôme, Abetz, López-Manchado and Vicario-Abejón. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Defteralı, Çağla Verdejo, Raquel Majeed, Shahid Boschetti-de-Fierro, Adriana Méndez-Gómez, Héctor R. Díaz-Guerra, Eva Fierro, Daniel Buhr, Kristian Abetz, Clarissa Martínez-Murillo, Ricardo Vuluga, Daniela Alexandre, Michaël Thomassin, Jean-Michel Detrembleur, Christophe Jérôme, Christine Abetz, Volker López-Manchado, Miguel Ángel Vicario-Abejón, Carlos In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia |
title | In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia |
title_full | In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia |
title_fullStr | In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia |
title_full_unstemmed | In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia |
title_short | In Vitro Evaluation of Biocompatibility of Uncoated Thermally Reduced Graphene and Carbon Nanotube-Loaded PVDF Membranes with Adult Neural Stem Cell-Derived Neurons and Glia |
title_sort | in vitro evaluation of biocompatibility of uncoated thermally reduced graphene and carbon nanotube-loaded pvdf membranes with adult neural stem cell-derived neurons and glia |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5138223/ https://www.ncbi.nlm.nih.gov/pubmed/27999773 http://dx.doi.org/10.3389/fbioe.2016.00094 |
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