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IL17-Producing γδ T Cells May Enhance Humoral Immunity during Pulmonary Pseudomonas aeruginosa Infection in Mice

The host acquired immune response, especially the humoral immunity, plays key roles in preventing bacterial pneumonia in the lung. Our previous research demonstrated that interleukin 17-producing γδ T cells (IL17-γδ T cells) have a protective effect on the early innate immune response during acute p...

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Autores principales: Pan, Tingting, Tan, Ruoming, Li, Meiling, Liu, Zhaojun, Wang, Xiaoli, Tian, Lijun, Liu, Jialin, Qu, Hongping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5138229/
https://www.ncbi.nlm.nih.gov/pubmed/27999768
http://dx.doi.org/10.3389/fcimb.2016.00170
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author Pan, Tingting
Tan, Ruoming
Li, Meiling
Liu, Zhaojun
Wang, Xiaoli
Tian, Lijun
Liu, Jialin
Qu, Hongping
author_facet Pan, Tingting
Tan, Ruoming
Li, Meiling
Liu, Zhaojun
Wang, Xiaoli
Tian, Lijun
Liu, Jialin
Qu, Hongping
author_sort Pan, Tingting
collection PubMed
description The host acquired immune response, especially the humoral immunity, plays key roles in preventing bacterial pneumonia in the lung. Our previous research demonstrated that interleukin 17-producing γδ T cells (IL17-γδ T cells) have a protective effect on the early innate immune response during acute pulmonary Pseudomonas aeruginosa infection. However, whether IL17-γδ T cells also play a role in humoral immunity is unknown. In this study, an acute pulmonary P. aeruginosa infection model was established in wild-type and γδ TCR(−/−) C57BL/6 mice. The expression of IL-17 on γδ T cells isolated from infected lung tissues increased rapidly and peaked at day 7 after acute infection with P. aeruginosa. Compared with wild-type infected mice, the levels of total immunoglobulins including IgA, IgG, and IgM in the serum and BALF were significantly decreased in γδ TCR(−/−) mice, with the exception of IgM in the BALF. Moreover, CD69 expression in B cells from the lungs and spleen and the level of BAFF in the plasma were also decreased in γδ TCR(−/−) mice. IL17-γδ T cell transfusion significantly improved the production of immunoglobulins, B cell activation and BAFF levels in γδ TCR(−/−) mice compared with γδ TCR(−/−) mice without transfusion; this effect was blocked when cells were pretreated with an IL-17 antibody. Together, these data demonstrate that IL17-γδ T cells are involved in CD19(+) B cell activation and the production of immunoglobulins during acute pulmonary P. aeruginosa infection. Thus, we conclude that IL17-γδ T cells may facilitate the elimination of bacteria and improve survival through not only innate immunity but also humoral immunity.
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spelling pubmed-51382292016-12-20 IL17-Producing γδ T Cells May Enhance Humoral Immunity during Pulmonary Pseudomonas aeruginosa Infection in Mice Pan, Tingting Tan, Ruoming Li, Meiling Liu, Zhaojun Wang, Xiaoli Tian, Lijun Liu, Jialin Qu, Hongping Front Cell Infect Microbiol Microbiology The host acquired immune response, especially the humoral immunity, plays key roles in preventing bacterial pneumonia in the lung. Our previous research demonstrated that interleukin 17-producing γδ T cells (IL17-γδ T cells) have a protective effect on the early innate immune response during acute pulmonary Pseudomonas aeruginosa infection. However, whether IL17-γδ T cells also play a role in humoral immunity is unknown. In this study, an acute pulmonary P. aeruginosa infection model was established in wild-type and γδ TCR(−/−) C57BL/6 mice. The expression of IL-17 on γδ T cells isolated from infected lung tissues increased rapidly and peaked at day 7 after acute infection with P. aeruginosa. Compared with wild-type infected mice, the levels of total immunoglobulins including IgA, IgG, and IgM in the serum and BALF were significantly decreased in γδ TCR(−/−) mice, with the exception of IgM in the BALF. Moreover, CD69 expression in B cells from the lungs and spleen and the level of BAFF in the plasma were also decreased in γδ TCR(−/−) mice. IL17-γδ T cell transfusion significantly improved the production of immunoglobulins, B cell activation and BAFF levels in γδ TCR(−/−) mice compared with γδ TCR(−/−) mice without transfusion; this effect was blocked when cells were pretreated with an IL-17 antibody. Together, these data demonstrate that IL17-γδ T cells are involved in CD19(+) B cell activation and the production of immunoglobulins during acute pulmonary P. aeruginosa infection. Thus, we conclude that IL17-γδ T cells may facilitate the elimination of bacteria and improve survival through not only innate immunity but also humoral immunity. Frontiers Media S.A. 2016-12-06 /pmc/articles/PMC5138229/ /pubmed/27999768 http://dx.doi.org/10.3389/fcimb.2016.00170 Text en Copyright © 2016 Pan, Tan, Li, Liu, Wang, Tian, Liu and Qu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Pan, Tingting
Tan, Ruoming
Li, Meiling
Liu, Zhaojun
Wang, Xiaoli
Tian, Lijun
Liu, Jialin
Qu, Hongping
IL17-Producing γδ T Cells May Enhance Humoral Immunity during Pulmonary Pseudomonas aeruginosa Infection in Mice
title IL17-Producing γδ T Cells May Enhance Humoral Immunity during Pulmonary Pseudomonas aeruginosa Infection in Mice
title_full IL17-Producing γδ T Cells May Enhance Humoral Immunity during Pulmonary Pseudomonas aeruginosa Infection in Mice
title_fullStr IL17-Producing γδ T Cells May Enhance Humoral Immunity during Pulmonary Pseudomonas aeruginosa Infection in Mice
title_full_unstemmed IL17-Producing γδ T Cells May Enhance Humoral Immunity during Pulmonary Pseudomonas aeruginosa Infection in Mice
title_short IL17-Producing γδ T Cells May Enhance Humoral Immunity during Pulmonary Pseudomonas aeruginosa Infection in Mice
title_sort il17-producing γδ t cells may enhance humoral immunity during pulmonary pseudomonas aeruginosa infection in mice
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5138229/
https://www.ncbi.nlm.nih.gov/pubmed/27999768
http://dx.doi.org/10.3389/fcimb.2016.00170
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