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Progressive Muscle Cell Delivery as a Solution for Volumetric Muscle Defect Repair

Reconstructing functional volumetric tissue in vivo following implantation remains a critical challenge facing cell-based approaches. Several pre-vascularization approaches have been developed to increase cell viability following implantation. Structural and functional restoration was achieved in a...

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Detalles Bibliográficos
Autores principales: Kim, Ji Hyun, Ko, In Kap, Atala, Anthony, Yoo, James J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5141432/
https://www.ncbi.nlm.nih.gov/pubmed/27924941
http://dx.doi.org/10.1038/srep38754
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author Kim, Ji Hyun
Ko, In Kap
Atala, Anthony
Yoo, James J.
author_facet Kim, Ji Hyun
Ko, In Kap
Atala, Anthony
Yoo, James J.
author_sort Kim, Ji Hyun
collection PubMed
description Reconstructing functional volumetric tissue in vivo following implantation remains a critical challenge facing cell-based approaches. Several pre-vascularization approaches have been developed to increase cell viability following implantation. Structural and functional restoration was achieved in a preclinical rodent tissue defect; however, the approach used in this model fails to repair larger (>mm) defects as observed in a clinical setting. We propose an effective cell delivery system utilizing appropriate vascularization at the site of cell implantation that results in volumetric and functional tissue reconstruction. Our method of multiple cell injections in a progressive manner yielded improved cell survival and formed volumetric muscle tissues in an ectopic muscle site. In addition, this strategy supported the reconstruction of functional skeletal muscle tissue in a rodent volumetric muscle loss injury model. Results from our study suggest that our method may be used to repair volumetric tissue defects by overcoming diffusion limitations and facilitating adequate vascularization.
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spelling pubmed-51414322016-12-16 Progressive Muscle Cell Delivery as a Solution for Volumetric Muscle Defect Repair Kim, Ji Hyun Ko, In Kap Atala, Anthony Yoo, James J. Sci Rep Article Reconstructing functional volumetric tissue in vivo following implantation remains a critical challenge facing cell-based approaches. Several pre-vascularization approaches have been developed to increase cell viability following implantation. Structural and functional restoration was achieved in a preclinical rodent tissue defect; however, the approach used in this model fails to repair larger (>mm) defects as observed in a clinical setting. We propose an effective cell delivery system utilizing appropriate vascularization at the site of cell implantation that results in volumetric and functional tissue reconstruction. Our method of multiple cell injections in a progressive manner yielded improved cell survival and formed volumetric muscle tissues in an ectopic muscle site. In addition, this strategy supported the reconstruction of functional skeletal muscle tissue in a rodent volumetric muscle loss injury model. Results from our study suggest that our method may be used to repair volumetric tissue defects by overcoming diffusion limitations and facilitating adequate vascularization. Nature Publishing Group 2016-12-07 /pmc/articles/PMC5141432/ /pubmed/27924941 http://dx.doi.org/10.1038/srep38754 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Kim, Ji Hyun
Ko, In Kap
Atala, Anthony
Yoo, James J.
Progressive Muscle Cell Delivery as a Solution for Volumetric Muscle Defect Repair
title Progressive Muscle Cell Delivery as a Solution for Volumetric Muscle Defect Repair
title_full Progressive Muscle Cell Delivery as a Solution for Volumetric Muscle Defect Repair
title_fullStr Progressive Muscle Cell Delivery as a Solution for Volumetric Muscle Defect Repair
title_full_unstemmed Progressive Muscle Cell Delivery as a Solution for Volumetric Muscle Defect Repair
title_short Progressive Muscle Cell Delivery as a Solution for Volumetric Muscle Defect Repair
title_sort progressive muscle cell delivery as a solution for volumetric muscle defect repair
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5141432/
https://www.ncbi.nlm.nih.gov/pubmed/27924941
http://dx.doi.org/10.1038/srep38754
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