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Cell studies of hybridized carbon nanofibers containing bioactive glass nanoparticles using bone mesenchymal stromal cells

Bone regeneration required suitable scaffolding materials to support the proliferation and osteogenic differentiation of bone-related cells. In this study, a kind of hybridized nanofibrous scaffold material (CNF/BG) was prepared by incorporating bioactive glass (BG) nanoparticles into carbon nanofib...

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Autores principales: Zhang, Xiu-Rui, Hu, Xiao-Qing, Jia, Xiao-Long, Yang, Li-Ka, Meng, Qing-Yang, Shi, Yuan-Yuan, Zhang, Zheng-Zheng, Cai, Qing, Ao, Yin-Fang, Yang, Xiao-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5141487/
https://www.ncbi.nlm.nih.gov/pubmed/27924854
http://dx.doi.org/10.1038/srep38685
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author Zhang, Xiu-Rui
Hu, Xiao-Qing
Jia, Xiao-Long
Yang, Li-Ka
Meng, Qing-Yang
Shi, Yuan-Yuan
Zhang, Zheng-Zheng
Cai, Qing
Ao, Yin-Fang
Yang, Xiao-Ping
author_facet Zhang, Xiu-Rui
Hu, Xiao-Qing
Jia, Xiao-Long
Yang, Li-Ka
Meng, Qing-Yang
Shi, Yuan-Yuan
Zhang, Zheng-Zheng
Cai, Qing
Ao, Yin-Fang
Yang, Xiao-Ping
author_sort Zhang, Xiu-Rui
collection PubMed
description Bone regeneration required suitable scaffolding materials to support the proliferation and osteogenic differentiation of bone-related cells. In this study, a kind of hybridized nanofibrous scaffold material (CNF/BG) was prepared by incorporating bioactive glass (BG) nanoparticles into carbon nanofibers (CNF) via the combination of BG sol-gel and polyacrylonitrile (PAN) electrospinning, followed by carbonization. Three types (49 s, 68 s and 86 s) of BG nanoparticles were incorporated. To understand the mechanism of CNF/BG hybrids exerting osteogenic effects, bone marrow mesenchymal stromal cells (BMSCs) were cultured directly on these hybrids (contact culture) or cultured in transwell chambers in the presence of these materials (non-contact culture). The contributions of ion release and contact effect on cell proliferation and osteogenic differentiation were able to be correlated. It was found that the ionic dissolution products had limited effect on cell proliferation, while they were able to enhance osteogenic differentiation of BMSCs in comparison with pure CNF. Differently, the proliferation and osteogenic differentiation were both significantly promoted in the contact culture. In both cases, CNF/BG(68 s) showed the strongest ability in influencing cell behaviors due to its fastest release rate of soluble silicium-relating ions. The synergistic effect of CNF and BG would make CNF/BG hybrids promising substrates for bone repairing.
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spelling pubmed-51414872016-12-16 Cell studies of hybridized carbon nanofibers containing bioactive glass nanoparticles using bone mesenchymal stromal cells Zhang, Xiu-Rui Hu, Xiao-Qing Jia, Xiao-Long Yang, Li-Ka Meng, Qing-Yang Shi, Yuan-Yuan Zhang, Zheng-Zheng Cai, Qing Ao, Yin-Fang Yang, Xiao-Ping Sci Rep Article Bone regeneration required suitable scaffolding materials to support the proliferation and osteogenic differentiation of bone-related cells. In this study, a kind of hybridized nanofibrous scaffold material (CNF/BG) was prepared by incorporating bioactive glass (BG) nanoparticles into carbon nanofibers (CNF) via the combination of BG sol-gel and polyacrylonitrile (PAN) electrospinning, followed by carbonization. Three types (49 s, 68 s and 86 s) of BG nanoparticles were incorporated. To understand the mechanism of CNF/BG hybrids exerting osteogenic effects, bone marrow mesenchymal stromal cells (BMSCs) were cultured directly on these hybrids (contact culture) or cultured in transwell chambers in the presence of these materials (non-contact culture). The contributions of ion release and contact effect on cell proliferation and osteogenic differentiation were able to be correlated. It was found that the ionic dissolution products had limited effect on cell proliferation, while they were able to enhance osteogenic differentiation of BMSCs in comparison with pure CNF. Differently, the proliferation and osteogenic differentiation were both significantly promoted in the contact culture. In both cases, CNF/BG(68 s) showed the strongest ability in influencing cell behaviors due to its fastest release rate of soluble silicium-relating ions. The synergistic effect of CNF and BG would make CNF/BG hybrids promising substrates for bone repairing. Nature Publishing Group 2016-12-07 /pmc/articles/PMC5141487/ /pubmed/27924854 http://dx.doi.org/10.1038/srep38685 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zhang, Xiu-Rui
Hu, Xiao-Qing
Jia, Xiao-Long
Yang, Li-Ka
Meng, Qing-Yang
Shi, Yuan-Yuan
Zhang, Zheng-Zheng
Cai, Qing
Ao, Yin-Fang
Yang, Xiao-Ping
Cell studies of hybridized carbon nanofibers containing bioactive glass nanoparticles using bone mesenchymal stromal cells
title Cell studies of hybridized carbon nanofibers containing bioactive glass nanoparticles using bone mesenchymal stromal cells
title_full Cell studies of hybridized carbon nanofibers containing bioactive glass nanoparticles using bone mesenchymal stromal cells
title_fullStr Cell studies of hybridized carbon nanofibers containing bioactive glass nanoparticles using bone mesenchymal stromal cells
title_full_unstemmed Cell studies of hybridized carbon nanofibers containing bioactive glass nanoparticles using bone mesenchymal stromal cells
title_short Cell studies of hybridized carbon nanofibers containing bioactive glass nanoparticles using bone mesenchymal stromal cells
title_sort cell studies of hybridized carbon nanofibers containing bioactive glass nanoparticles using bone mesenchymal stromal cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5141487/
https://www.ncbi.nlm.nih.gov/pubmed/27924854
http://dx.doi.org/10.1038/srep38685
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