AB275. SPR-02 Influence of mucosal remodeling on urothelial functions

OBJECTIVE: Lower urinary tract symptoms (LUTS), in particular storage symptoms are a major health related problem. Much of research past and present, has focused on detrusor muscle function and changes in the central neurological control of LUT function; however, much less is known about the role of the urothelium and cells within the lamina propria in these events. Our research has shown that decreased vascular perfusion and changes within the extracellular matrix (ECM) can significantly impact urothelial signaling. METHODS: These include microangiography (for vascular imaging) as well as concurrent (multi-photon) imaging and mechanical testing of collagen fibers in the bladder wall of the rat. RESULTS AND CONCLUSIONS: Besides sensing chemical signals, the urothelium is highly responsive to mechanical loads thus the mechanical properties of the lamina propria and associated ECM may an important role in its function. Thus, changes in release of mediators and fiber architecture with age or bladder pathology can adversely impact the mechanobiology and hence sensation and bladder function. FUNDING SOURCE(S): NIH