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Chrysophanic Acid Suppresses Adipogenesis and Induces Thermogenesis by Activating AMP-Activated Protein Kinase Alpha In vivo and In vitro

Chrysophanic acid (CA) is a member of the anthraquinone family abundant in rhubarb, a widely used herb for obesity treatment in Traditional Korean Medicine. Though several studies have indicated numerous features of CA, no study has yet reported the effect of CA on obesity. In this study, we tried t...

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Autores principales: Lim, Hara, Park, Jinbong, Kim, Hye-Lin, Kang, JongWook, Jeong, Mi-Young, Youn, Dong-Hyun, Jung, Yunu, Kim, Yong-Il, Kim, Hyun-Ju, Ahn, Kwang Seok, Kim, Su-Jin, Choe, Seong-Kyu, Hong, Seung-Heon, Um, Jae-Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5143616/
https://www.ncbi.nlm.nih.gov/pubmed/28008317
http://dx.doi.org/10.3389/fphar.2016.00476
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author Lim, Hara
Park, Jinbong
Kim, Hye-Lin
Kang, JongWook
Jeong, Mi-Young
Youn, Dong-Hyun
Jung, Yunu
Kim, Yong-Il
Kim, Hyun-Ju
Ahn, Kwang Seok
Kim, Su-Jin
Choe, Seong-Kyu
Hong, Seung-Heon
Um, Jae-Young
author_facet Lim, Hara
Park, Jinbong
Kim, Hye-Lin
Kang, JongWook
Jeong, Mi-Young
Youn, Dong-Hyun
Jung, Yunu
Kim, Yong-Il
Kim, Hyun-Ju
Ahn, Kwang Seok
Kim, Su-Jin
Choe, Seong-Kyu
Hong, Seung-Heon
Um, Jae-Young
author_sort Lim, Hara
collection PubMed
description Chrysophanic acid (CA) is a member of the anthraquinone family abundant in rhubarb, a widely used herb for obesity treatment in Traditional Korean Medicine. Though several studies have indicated numerous features of CA, no study has yet reported the effect of CA on obesity. In this study, we tried to identify the anti-obesity effects of CA. By using 3T3-L1 adipocytes and primary cultured brown adipocytes as in vitro models, high-fat diet (HFD)-induced obese mice, and zebrafish as in vivo models, we determined the anti-obesity effects of CA. CA reduced weight gain in HFD-induced obese mice. They also decreased lipid accumulation and the expressions of adipogenesis factors including peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein alpha (C/EBPα) in 3T3-L1 adipocytes. In addition, uncoupling protein 1 (UCP1) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α), the brown fat specific thermogenic genes, were up-regulated in brown adipocytes by CA treatment. Furthermore, when co-treated with Compound C, the AMP-activated protein kinase (AMPK) inhibitor, the action of CA on AMPKα was nullified in both types of adipocytes, indicating the multi-controlling effect of CA was partially via the AMPKα pathway. Given all together, these results indicate that CA can ameliorate obesity by controlling the adipogenic and thermogenic pathway at the same time. On these bases, we suggest the new potential of CA as an anti-obese pharmacotherapy.
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spelling pubmed-51436162016-12-22 Chrysophanic Acid Suppresses Adipogenesis and Induces Thermogenesis by Activating AMP-Activated Protein Kinase Alpha In vivo and In vitro Lim, Hara Park, Jinbong Kim, Hye-Lin Kang, JongWook Jeong, Mi-Young Youn, Dong-Hyun Jung, Yunu Kim, Yong-Il Kim, Hyun-Ju Ahn, Kwang Seok Kim, Su-Jin Choe, Seong-Kyu Hong, Seung-Heon Um, Jae-Young Front Pharmacol Pharmacology Chrysophanic acid (CA) is a member of the anthraquinone family abundant in rhubarb, a widely used herb for obesity treatment in Traditional Korean Medicine. Though several studies have indicated numerous features of CA, no study has yet reported the effect of CA on obesity. In this study, we tried to identify the anti-obesity effects of CA. By using 3T3-L1 adipocytes and primary cultured brown adipocytes as in vitro models, high-fat diet (HFD)-induced obese mice, and zebrafish as in vivo models, we determined the anti-obesity effects of CA. CA reduced weight gain in HFD-induced obese mice. They also decreased lipid accumulation and the expressions of adipogenesis factors including peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein alpha (C/EBPα) in 3T3-L1 adipocytes. In addition, uncoupling protein 1 (UCP1) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α), the brown fat specific thermogenic genes, were up-regulated in brown adipocytes by CA treatment. Furthermore, when co-treated with Compound C, the AMP-activated protein kinase (AMPK) inhibitor, the action of CA on AMPKα was nullified in both types of adipocytes, indicating the multi-controlling effect of CA was partially via the AMPKα pathway. Given all together, these results indicate that CA can ameliorate obesity by controlling the adipogenic and thermogenic pathway at the same time. On these bases, we suggest the new potential of CA as an anti-obese pharmacotherapy. Frontiers Media S.A. 2016-12-08 /pmc/articles/PMC5143616/ /pubmed/28008317 http://dx.doi.org/10.3389/fphar.2016.00476 Text en Copyright © 2016 Lim, Park, Kim, Kang, Jeong, Youn, Jung, Kim, Kim, Ahn, Kim, Choe, Hong and Um. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Lim, Hara
Park, Jinbong
Kim, Hye-Lin
Kang, JongWook
Jeong, Mi-Young
Youn, Dong-Hyun
Jung, Yunu
Kim, Yong-Il
Kim, Hyun-Ju
Ahn, Kwang Seok
Kim, Su-Jin
Choe, Seong-Kyu
Hong, Seung-Heon
Um, Jae-Young
Chrysophanic Acid Suppresses Adipogenesis and Induces Thermogenesis by Activating AMP-Activated Protein Kinase Alpha In vivo and In vitro
title Chrysophanic Acid Suppresses Adipogenesis and Induces Thermogenesis by Activating AMP-Activated Protein Kinase Alpha In vivo and In vitro
title_full Chrysophanic Acid Suppresses Adipogenesis and Induces Thermogenesis by Activating AMP-Activated Protein Kinase Alpha In vivo and In vitro
title_fullStr Chrysophanic Acid Suppresses Adipogenesis and Induces Thermogenesis by Activating AMP-Activated Protein Kinase Alpha In vivo and In vitro
title_full_unstemmed Chrysophanic Acid Suppresses Adipogenesis and Induces Thermogenesis by Activating AMP-Activated Protein Kinase Alpha In vivo and In vitro
title_short Chrysophanic Acid Suppresses Adipogenesis and Induces Thermogenesis by Activating AMP-Activated Protein Kinase Alpha In vivo and In vitro
title_sort chrysophanic acid suppresses adipogenesis and induces thermogenesis by activating amp-activated protein kinase alpha in vivo and in vitro
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5143616/
https://www.ncbi.nlm.nih.gov/pubmed/28008317
http://dx.doi.org/10.3389/fphar.2016.00476
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