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The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato
The physiological functions of sphingolipids in animals have been intensively studied, while less attention has been paid to their roles in plants. Here, we reveal the involvement of sphingolipid delta8 desaturase (SlSLD) in the chilling resistance of tomato (Solanum lycopersicum cv. Micro-Tom). We...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5143999/ https://www.ncbi.nlm.nih.gov/pubmed/27929095 http://dx.doi.org/10.1038/srep38742 |
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author | Zhou, Ying Zeng, Lanting Fu, Xiumin Mei, Xin Cheng, Sihua Liao, Yinyin Deng, Rufang Xu, Xinlan Jiang, Yueming Duan, Xuewu Baldermann, Susanne Yang, Ziyin |
author_facet | Zhou, Ying Zeng, Lanting Fu, Xiumin Mei, Xin Cheng, Sihua Liao, Yinyin Deng, Rufang Xu, Xinlan Jiang, Yueming Duan, Xuewu Baldermann, Susanne Yang, Ziyin |
author_sort | Zhou, Ying |
collection | PubMed |
description | The physiological functions of sphingolipids in animals have been intensively studied, while less attention has been paid to their roles in plants. Here, we reveal the involvement of sphingolipid delta8 desaturase (SlSLD) in the chilling resistance of tomato (Solanum lycopersicum cv. Micro-Tom). We used the virus-induced gene silencing (VIGS) approach to knock-down SlSLD expression in tomato leaves, and then evaluated chilling resistance. Changes in leaf cell structure under a chilling treatment were observed by transmission electron microscopy. In control plants, SlSLD was highly expressed in the fruit and leaves in response to a chilling treatment. The degree of chilling damage was greater in SlSLD-silenced plants than in control plants, indicating that SlSLD knock-down significantly reduced the chilling resistance of tomato. Compared with control plants, SlSLD-silenced plants showed higher relative electrolytic leakage and malondialdehyde content, and lower superoxide dismutase and peroxidase activities after a chilling treatment. Chilling severely damaged the chloroplasts in SlSLD-silenced plants, resulting in the disruption of chloroplast membranes, swelling of thylakoids, and reduced granal stacking. Together, these results show that SlSLD is crucial for chilling resistance in tomato. |
format | Online Article Text |
id | pubmed-5143999 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-51439992016-12-16 The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato Zhou, Ying Zeng, Lanting Fu, Xiumin Mei, Xin Cheng, Sihua Liao, Yinyin Deng, Rufang Xu, Xinlan Jiang, Yueming Duan, Xuewu Baldermann, Susanne Yang, Ziyin Sci Rep Article The physiological functions of sphingolipids in animals have been intensively studied, while less attention has been paid to their roles in plants. Here, we reveal the involvement of sphingolipid delta8 desaturase (SlSLD) in the chilling resistance of tomato (Solanum lycopersicum cv. Micro-Tom). We used the virus-induced gene silencing (VIGS) approach to knock-down SlSLD expression in tomato leaves, and then evaluated chilling resistance. Changes in leaf cell structure under a chilling treatment were observed by transmission electron microscopy. In control plants, SlSLD was highly expressed in the fruit and leaves in response to a chilling treatment. The degree of chilling damage was greater in SlSLD-silenced plants than in control plants, indicating that SlSLD knock-down significantly reduced the chilling resistance of tomato. Compared with control plants, SlSLD-silenced plants showed higher relative electrolytic leakage and malondialdehyde content, and lower superoxide dismutase and peroxidase activities after a chilling treatment. Chilling severely damaged the chloroplasts in SlSLD-silenced plants, resulting in the disruption of chloroplast membranes, swelling of thylakoids, and reduced granal stacking. Together, these results show that SlSLD is crucial for chilling resistance in tomato. Nature Publishing Group 2016-12-08 /pmc/articles/PMC5143999/ /pubmed/27929095 http://dx.doi.org/10.1038/srep38742 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Zhou, Ying Zeng, Lanting Fu, Xiumin Mei, Xin Cheng, Sihua Liao, Yinyin Deng, Rufang Xu, Xinlan Jiang, Yueming Duan, Xuewu Baldermann, Susanne Yang, Ziyin The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato |
title | The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato |
title_full | The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato |
title_fullStr | The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato |
title_full_unstemmed | The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato |
title_short | The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato |
title_sort | sphingolipid biosynthetic enzyme sphingolipid delta8 desaturase is important for chilling resistance of tomato |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5143999/ https://www.ncbi.nlm.nih.gov/pubmed/27929095 http://dx.doi.org/10.1038/srep38742 |
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