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The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato

The physiological functions of sphingolipids in animals have been intensively studied, while less attention has been paid to their roles in plants. Here, we reveal the involvement of sphingolipid delta8 desaturase (SlSLD) in the chilling resistance of tomato (Solanum lycopersicum cv. Micro-Tom). We...

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Autores principales: Zhou, Ying, Zeng, Lanting, Fu, Xiumin, Mei, Xin, Cheng, Sihua, Liao, Yinyin, Deng, Rufang, Xu, Xinlan, Jiang, Yueming, Duan, Xuewu, Baldermann, Susanne, Yang, Ziyin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5143999/
https://www.ncbi.nlm.nih.gov/pubmed/27929095
http://dx.doi.org/10.1038/srep38742
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author Zhou, Ying
Zeng, Lanting
Fu, Xiumin
Mei, Xin
Cheng, Sihua
Liao, Yinyin
Deng, Rufang
Xu, Xinlan
Jiang, Yueming
Duan, Xuewu
Baldermann, Susanne
Yang, Ziyin
author_facet Zhou, Ying
Zeng, Lanting
Fu, Xiumin
Mei, Xin
Cheng, Sihua
Liao, Yinyin
Deng, Rufang
Xu, Xinlan
Jiang, Yueming
Duan, Xuewu
Baldermann, Susanne
Yang, Ziyin
author_sort Zhou, Ying
collection PubMed
description The physiological functions of sphingolipids in animals have been intensively studied, while less attention has been paid to their roles in plants. Here, we reveal the involvement of sphingolipid delta8 desaturase (SlSLD) in the chilling resistance of tomato (Solanum lycopersicum cv. Micro-Tom). We used the virus-induced gene silencing (VIGS) approach to knock-down SlSLD expression in tomato leaves, and then evaluated chilling resistance. Changes in leaf cell structure under a chilling treatment were observed by transmission electron microscopy. In control plants, SlSLD was highly expressed in the fruit and leaves in response to a chilling treatment. The degree of chilling damage was greater in SlSLD-silenced plants than in control plants, indicating that SlSLD knock-down significantly reduced the chilling resistance of tomato. Compared with control plants, SlSLD-silenced plants showed higher relative electrolytic leakage and malondialdehyde content, and lower superoxide dismutase and peroxidase activities after a chilling treatment. Chilling severely damaged the chloroplasts in SlSLD-silenced plants, resulting in the disruption of chloroplast membranes, swelling of thylakoids, and reduced granal stacking. Together, these results show that SlSLD is crucial for chilling resistance in tomato.
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spelling pubmed-51439992016-12-16 The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato Zhou, Ying Zeng, Lanting Fu, Xiumin Mei, Xin Cheng, Sihua Liao, Yinyin Deng, Rufang Xu, Xinlan Jiang, Yueming Duan, Xuewu Baldermann, Susanne Yang, Ziyin Sci Rep Article The physiological functions of sphingolipids in animals have been intensively studied, while less attention has been paid to their roles in plants. Here, we reveal the involvement of sphingolipid delta8 desaturase (SlSLD) in the chilling resistance of tomato (Solanum lycopersicum cv. Micro-Tom). We used the virus-induced gene silencing (VIGS) approach to knock-down SlSLD expression in tomato leaves, and then evaluated chilling resistance. Changes in leaf cell structure under a chilling treatment were observed by transmission electron microscopy. In control plants, SlSLD was highly expressed in the fruit and leaves in response to a chilling treatment. The degree of chilling damage was greater in SlSLD-silenced plants than in control plants, indicating that SlSLD knock-down significantly reduced the chilling resistance of tomato. Compared with control plants, SlSLD-silenced plants showed higher relative electrolytic leakage and malondialdehyde content, and lower superoxide dismutase and peroxidase activities after a chilling treatment. Chilling severely damaged the chloroplasts in SlSLD-silenced plants, resulting in the disruption of chloroplast membranes, swelling of thylakoids, and reduced granal stacking. Together, these results show that SlSLD is crucial for chilling resistance in tomato. Nature Publishing Group 2016-12-08 /pmc/articles/PMC5143999/ /pubmed/27929095 http://dx.doi.org/10.1038/srep38742 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zhou, Ying
Zeng, Lanting
Fu, Xiumin
Mei, Xin
Cheng, Sihua
Liao, Yinyin
Deng, Rufang
Xu, Xinlan
Jiang, Yueming
Duan, Xuewu
Baldermann, Susanne
Yang, Ziyin
The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato
title The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato
title_full The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato
title_fullStr The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato
title_full_unstemmed The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato
title_short The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato
title_sort sphingolipid biosynthetic enzyme sphingolipid delta8 desaturase is important for chilling resistance of tomato
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5143999/
https://www.ncbi.nlm.nih.gov/pubmed/27929095
http://dx.doi.org/10.1038/srep38742
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