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Acfs: accurate circRNA identification and quantification from RNA-Seq data
Circular RNAs (circRNAs) are a group of single-stranded RNAs in closed circular form. They are splicing-generated, widely expressed in various tissues and have functional implications in development and diseases. To facilitate genome-wide characterization of circRNAs using RNA-Seq data, we present a...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5144000/ https://www.ncbi.nlm.nih.gov/pubmed/27929140 http://dx.doi.org/10.1038/srep38820 |
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author | You, Xintian Conrad, Tim OF |
author_facet | You, Xintian Conrad, Tim OF |
author_sort | You, Xintian |
collection | PubMed |
description | Circular RNAs (circRNAs) are a group of single-stranded RNAs in closed circular form. They are splicing-generated, widely expressed in various tissues and have functional implications in development and diseases. To facilitate genome-wide characterization of circRNAs using RNA-Seq data, we present a freely available software package named acfs. Acfs allows de novo, accurate and fast identification and abundance quantification of circRNAs from single- and paired-ended RNA-Seq data. On simulated datasets, acfs achieved the highest F1 accuracy and lowest false discovery rate among current state-of-the-art tools. On real-world datasets, acfs efficiently identified more bona fide circRNAs. Furthermore, we demonstrated the power of circRNA analysis on two leukemia datasets. We identified a set of circRNAs that are differentially expressed between AML and APL samples, which might shed light on the potential molecular classification of complex diseases using circRNA profiles. Moreover, chromosomal translocation, as manifested in numerous diseases, could produce not only fusion transcripts but also fusion circRNAs of clinical relevance. Featured with high accuracy, low FDR and the ability to identify fusion circRNAs, we believe that acfs is well suited for a wide spectrum of applications in characterizing the landscape of circRNAs from non-model organisms to cancer biology. |
format | Online Article Text |
id | pubmed-5144000 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-51440002016-12-16 Acfs: accurate circRNA identification and quantification from RNA-Seq data You, Xintian Conrad, Tim OF Sci Rep Article Circular RNAs (circRNAs) are a group of single-stranded RNAs in closed circular form. They are splicing-generated, widely expressed in various tissues and have functional implications in development and diseases. To facilitate genome-wide characterization of circRNAs using RNA-Seq data, we present a freely available software package named acfs. Acfs allows de novo, accurate and fast identification and abundance quantification of circRNAs from single- and paired-ended RNA-Seq data. On simulated datasets, acfs achieved the highest F1 accuracy and lowest false discovery rate among current state-of-the-art tools. On real-world datasets, acfs efficiently identified more bona fide circRNAs. Furthermore, we demonstrated the power of circRNA analysis on two leukemia datasets. We identified a set of circRNAs that are differentially expressed between AML and APL samples, which might shed light on the potential molecular classification of complex diseases using circRNA profiles. Moreover, chromosomal translocation, as manifested in numerous diseases, could produce not only fusion transcripts but also fusion circRNAs of clinical relevance. Featured with high accuracy, low FDR and the ability to identify fusion circRNAs, we believe that acfs is well suited for a wide spectrum of applications in characterizing the landscape of circRNAs from non-model organisms to cancer biology. Nature Publishing Group 2016-12-08 /pmc/articles/PMC5144000/ /pubmed/27929140 http://dx.doi.org/10.1038/srep38820 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article You, Xintian Conrad, Tim OF Acfs: accurate circRNA identification and quantification from RNA-Seq data |
title | Acfs: accurate circRNA identification and quantification from RNA-Seq data |
title_full | Acfs: accurate circRNA identification and quantification from RNA-Seq data |
title_fullStr | Acfs: accurate circRNA identification and quantification from RNA-Seq data |
title_full_unstemmed | Acfs: accurate circRNA identification and quantification from RNA-Seq data |
title_short | Acfs: accurate circRNA identification and quantification from RNA-Seq data |
title_sort | acfs: accurate circrna identification and quantification from rna-seq data |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5144000/ https://www.ncbi.nlm.nih.gov/pubmed/27929140 http://dx.doi.org/10.1038/srep38820 |
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