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Glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma
BACKGROUND: The incidence of liver disease is increasing in USA. Animal models had shown glutathione species in plasma reflects liver glutathione state and it could be a surrogate for the detection of hepatocellular carcinoma (HCC). METHODS: The present study aimed to translate methods to the human...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5144552/ https://www.ncbi.nlm.nih.gov/pubmed/28340971 http://dx.doi.org/10.1016/j.hpb.2016.09.007 |
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author | Sanabria, Juan R. Kombu, Rajan S. Zhang, Guo-Fang Sandlers, Yana Ai, Jizhou Ibarra, Rafael A. Abbas, Rime Goyal, Kush Brunengraber, Henri |
author_facet | Sanabria, Juan R. Kombu, Rajan S. Zhang, Guo-Fang Sandlers, Yana Ai, Jizhou Ibarra, Rafael A. Abbas, Rime Goyal, Kush Brunengraber, Henri |
author_sort | Sanabria, Juan R. |
collection | PubMed |
description | BACKGROUND: The incidence of liver disease is increasing in USA. Animal models had shown glutathione species in plasma reflects liver glutathione state and it could be a surrogate for the detection of hepatocellular carcinoma (HCC). METHODS: The present study aimed to translate methods to the human and to explore the role of glutathione/metabolic prints in the progression of liver dysfunction and in the detection of HCC. Treated plasma from healthy subjects (n = 20), patients with liver disease (ESLD, n = 99) and patients after transplantation (LTx, n = 7) were analyzed by GC- or LC/MS. Glutathione labeling profile was measured by isotopomer analyzes of (2)H(2)O enriched plasma. Principal Component Analyzes (PCA) were used to determined metabolic prints. RESULTS: There was a significant difference in glutathione/metabolic profiles from patients with ESLD vs healthy subjects and patients after LTx. Similar significant differences were noted on patients with ESLD when stratified by the MELD score. PCA analyses showed myristic acid, citric acid, succinic acid, l-methionine, d-threitol, fumaric acid, pipecolic acid, isoleucine, hydroxy-butyrate and glycolic, steraric and hexanoic acids were discriminative metabolites for ESLD-HCC(+) vs ESLD-HCC(−) subject status. CONCLUSIONS: Glutathione species and metabolic prints defined liver disease severity and may serve as surrogate for the detection of HCC in patients with established cirrhosis. |
format | Online Article Text |
id | pubmed-5144552 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-51445522016-12-12 Glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma Sanabria, Juan R. Kombu, Rajan S. Zhang, Guo-Fang Sandlers, Yana Ai, Jizhou Ibarra, Rafael A. Abbas, Rime Goyal, Kush Brunengraber, Henri HPB (Oxford) Original Article BACKGROUND: The incidence of liver disease is increasing in USA. Animal models had shown glutathione species in plasma reflects liver glutathione state and it could be a surrogate for the detection of hepatocellular carcinoma (HCC). METHODS: The present study aimed to translate methods to the human and to explore the role of glutathione/metabolic prints in the progression of liver dysfunction and in the detection of HCC. Treated plasma from healthy subjects (n = 20), patients with liver disease (ESLD, n = 99) and patients after transplantation (LTx, n = 7) were analyzed by GC- or LC/MS. Glutathione labeling profile was measured by isotopomer analyzes of (2)H(2)O enriched plasma. Principal Component Analyzes (PCA) were used to determined metabolic prints. RESULTS: There was a significant difference in glutathione/metabolic profiles from patients with ESLD vs healthy subjects and patients after LTx. Similar significant differences were noted on patients with ESLD when stratified by the MELD score. PCA analyses showed myristic acid, citric acid, succinic acid, l-methionine, d-threitol, fumaric acid, pipecolic acid, isoleucine, hydroxy-butyrate and glycolic, steraric and hexanoic acids were discriminative metabolites for ESLD-HCC(+) vs ESLD-HCC(−) subject status. CONCLUSIONS: Glutathione species and metabolic prints defined liver disease severity and may serve as surrogate for the detection of HCC in patients with established cirrhosis. Elsevier 2016-12 2016-10-27 /pmc/articles/PMC5144552/ /pubmed/28340971 http://dx.doi.org/10.1016/j.hpb.2016.09.007 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Sanabria, Juan R. Kombu, Rajan S. Zhang, Guo-Fang Sandlers, Yana Ai, Jizhou Ibarra, Rafael A. Abbas, Rime Goyal, Kush Brunengraber, Henri Glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma |
title | Glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma |
title_full | Glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma |
title_fullStr | Glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma |
title_full_unstemmed | Glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma |
title_short | Glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma |
title_sort | glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5144552/ https://www.ncbi.nlm.nih.gov/pubmed/28340971 http://dx.doi.org/10.1016/j.hpb.2016.09.007 |
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