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Glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma

BACKGROUND: The incidence of liver disease is increasing in USA. Animal models had shown glutathione species in plasma reflects liver glutathione state and it could be a surrogate for the detection of hepatocellular carcinoma (HCC). METHODS: The present study aimed to translate methods to the human...

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Autores principales: Sanabria, Juan R., Kombu, Rajan S., Zhang, Guo-Fang, Sandlers, Yana, Ai, Jizhou, Ibarra, Rafael A., Abbas, Rime, Goyal, Kush, Brunengraber, Henri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5144552/
https://www.ncbi.nlm.nih.gov/pubmed/28340971
http://dx.doi.org/10.1016/j.hpb.2016.09.007
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author Sanabria, Juan R.
Kombu, Rajan S.
Zhang, Guo-Fang
Sandlers, Yana
Ai, Jizhou
Ibarra, Rafael A.
Abbas, Rime
Goyal, Kush
Brunengraber, Henri
author_facet Sanabria, Juan R.
Kombu, Rajan S.
Zhang, Guo-Fang
Sandlers, Yana
Ai, Jizhou
Ibarra, Rafael A.
Abbas, Rime
Goyal, Kush
Brunengraber, Henri
author_sort Sanabria, Juan R.
collection PubMed
description BACKGROUND: The incidence of liver disease is increasing in USA. Animal models had shown glutathione species in plasma reflects liver glutathione state and it could be a surrogate for the detection of hepatocellular carcinoma (HCC). METHODS: The present study aimed to translate methods to the human and to explore the role of glutathione/metabolic prints in the progression of liver dysfunction and in the detection of HCC. Treated plasma from healthy subjects (n = 20), patients with liver disease (ESLD, n = 99) and patients after transplantation (LTx, n = 7) were analyzed by GC- or LC/MS. Glutathione labeling profile was measured by isotopomer analyzes of (2)H(2)O enriched plasma. Principal Component Analyzes (PCA) were used to determined metabolic prints. RESULTS: There was a significant difference in glutathione/metabolic profiles from patients with ESLD vs healthy subjects and patients after LTx. Similar significant differences were noted on patients with ESLD when stratified by the MELD score. PCA analyses showed myristic acid, citric acid, succinic acid, l-methionine, d-threitol, fumaric acid, pipecolic acid, isoleucine, hydroxy-butyrate and glycolic, steraric and hexanoic acids were discriminative metabolites for ESLD-HCC(+) vs ESLD-HCC(−) subject status. CONCLUSIONS: Glutathione species and metabolic prints defined liver disease severity and may serve as surrogate for the detection of HCC in patients with established cirrhosis.
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spelling pubmed-51445522016-12-12 Glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma Sanabria, Juan R. Kombu, Rajan S. Zhang, Guo-Fang Sandlers, Yana Ai, Jizhou Ibarra, Rafael A. Abbas, Rime Goyal, Kush Brunengraber, Henri HPB (Oxford) Original Article BACKGROUND: The incidence of liver disease is increasing in USA. Animal models had shown glutathione species in plasma reflects liver glutathione state and it could be a surrogate for the detection of hepatocellular carcinoma (HCC). METHODS: The present study aimed to translate methods to the human and to explore the role of glutathione/metabolic prints in the progression of liver dysfunction and in the detection of HCC. Treated plasma from healthy subjects (n = 20), patients with liver disease (ESLD, n = 99) and patients after transplantation (LTx, n = 7) were analyzed by GC- or LC/MS. Glutathione labeling profile was measured by isotopomer analyzes of (2)H(2)O enriched plasma. Principal Component Analyzes (PCA) were used to determined metabolic prints. RESULTS: There was a significant difference in glutathione/metabolic profiles from patients with ESLD vs healthy subjects and patients after LTx. Similar significant differences were noted on patients with ESLD when stratified by the MELD score. PCA analyses showed myristic acid, citric acid, succinic acid, l-methionine, d-threitol, fumaric acid, pipecolic acid, isoleucine, hydroxy-butyrate and glycolic, steraric and hexanoic acids were discriminative metabolites for ESLD-HCC(+) vs ESLD-HCC(−) subject status. CONCLUSIONS: Glutathione species and metabolic prints defined liver disease severity and may serve as surrogate for the detection of HCC in patients with established cirrhosis. Elsevier 2016-12 2016-10-27 /pmc/articles/PMC5144552/ /pubmed/28340971 http://dx.doi.org/10.1016/j.hpb.2016.09.007 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Sanabria, Juan R.
Kombu, Rajan S.
Zhang, Guo-Fang
Sandlers, Yana
Ai, Jizhou
Ibarra, Rafael A.
Abbas, Rime
Goyal, Kush
Brunengraber, Henri
Glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma
title Glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma
title_full Glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma
title_fullStr Glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma
title_full_unstemmed Glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma
title_short Glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma
title_sort glutathione species and metabolomic prints in subjects with liver disease as biological markers for the detection of hepatocellular carcinoma
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5144552/
https://www.ncbi.nlm.nih.gov/pubmed/28340971
http://dx.doi.org/10.1016/j.hpb.2016.09.007
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