Cargando…

9.4 T MR microscopy of the substantia nigra with pathological validation in controls and disease

BACKGROUND: The anatomy of the substantia nigra on conventional MRI is controversial. Even using histological techniques it is difficult to delineate with certainty from surrounding structures. We sought to define the anatomy of the SN using high field spin-echo MRI of pathological material in which...

Descripción completa

Detalles Bibliográficos
Autores principales: Massey, LA, Miranda, MA, Al-Helli, O, Parkes, HG, Thornton, JS, So, P-W, White, MJ, Mancini, L, Strand, C, Holton, J, Lees, AJ, Revesz, T, Yousry, TA
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5144755/
https://www.ncbi.nlm.nih.gov/pubmed/27981030
http://dx.doi.org/10.1016/j.nicl.2016.11.015
_version_ 1782473174474031104
author Massey, LA
Miranda, MA
Al-Helli, O
Parkes, HG
Thornton, JS
So, P-W
White, MJ
Mancini, L
Strand, C
Holton, J
Lees, AJ
Revesz, T
Yousry, TA
author_facet Massey, LA
Miranda, MA
Al-Helli, O
Parkes, HG
Thornton, JS
So, P-W
White, MJ
Mancini, L
Strand, C
Holton, J
Lees, AJ
Revesz, T
Yousry, TA
author_sort Massey, LA
collection PubMed
description BACKGROUND: The anatomy of the substantia nigra on conventional MRI is controversial. Even using histological techniques it is difficult to delineate with certainty from surrounding structures. We sought to define the anatomy of the SN using high field spin-echo MRI of pathological material in which we could study the anatomy in detail to corroborate our MRI findings in controls and Parkinson's disease and progressive supranuclear palsy. METHODS: 23 brains were selected from the Queen Square Brain Bank (10 controls, 8 progressive supranuclear palsy, 5 Parkinson's disease) and imaged using high field 9.4 Tesla spin-echo MRI. Subsequently brains were cut and stained with Luxol fast blue, Perls stain, and immunohistochemistry for substance P and calbindin. Once the anatomy was defined on histology the dimensions and volume of the substantia nigra were determined on high field magnetic resonance images. RESULTS: The anterior border of the substantia nigra was defined by the crus cerebri. In the medial half it was less distinct due to the deposition of iron and the interdigitation of white matter and the substantia nigra. The posterior border was flanked by white matter bridging the red nucleus and substantia nigra and seen as hypointense on spin-echo magnetic resonance images. Within the substantia nigra high signal structures corresponded to confirmed nigrosomes. These were still evident in Parkinson's disease but not in progressive supranuclear palsy. The volume and dimensions of the substantia nigra were similar in Parkinson's disease and controls, but reduced in progressive supranuclear palsy. CONCLUSIONS: We present a histologically validated anatomical description of the substantia nigra on high field spin-echo high resolution magnetic resonance images and were able to delineate all five nigrosomes. In accordance with the pathological literature we did not observe changes in the nigrosome structure as manifest by volume or signal characteristics within the substantia nigra in Parkinson's disease whereas in progressive supranuclear palsy there was microarchitectural destruction.
format Online
Article
Text
id pubmed-5144755
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-51447552016-12-15 9.4 T MR microscopy of the substantia nigra with pathological validation in controls and disease Massey, LA Miranda, MA Al-Helli, O Parkes, HG Thornton, JS So, P-W White, MJ Mancini, L Strand, C Holton, J Lees, AJ Revesz, T Yousry, TA Neuroimage Clin Regular Article BACKGROUND: The anatomy of the substantia nigra on conventional MRI is controversial. Even using histological techniques it is difficult to delineate with certainty from surrounding structures. We sought to define the anatomy of the SN using high field spin-echo MRI of pathological material in which we could study the anatomy in detail to corroborate our MRI findings in controls and Parkinson's disease and progressive supranuclear palsy. METHODS: 23 brains were selected from the Queen Square Brain Bank (10 controls, 8 progressive supranuclear palsy, 5 Parkinson's disease) and imaged using high field 9.4 Tesla spin-echo MRI. Subsequently brains were cut and stained with Luxol fast blue, Perls stain, and immunohistochemistry for substance P and calbindin. Once the anatomy was defined on histology the dimensions and volume of the substantia nigra were determined on high field magnetic resonance images. RESULTS: The anterior border of the substantia nigra was defined by the crus cerebri. In the medial half it was less distinct due to the deposition of iron and the interdigitation of white matter and the substantia nigra. The posterior border was flanked by white matter bridging the red nucleus and substantia nigra and seen as hypointense on spin-echo magnetic resonance images. Within the substantia nigra high signal structures corresponded to confirmed nigrosomes. These were still evident in Parkinson's disease but not in progressive supranuclear palsy. The volume and dimensions of the substantia nigra were similar in Parkinson's disease and controls, but reduced in progressive supranuclear palsy. CONCLUSIONS: We present a histologically validated anatomical description of the substantia nigra on high field spin-echo high resolution magnetic resonance images and were able to delineate all five nigrosomes. In accordance with the pathological literature we did not observe changes in the nigrosome structure as manifest by volume or signal characteristics within the substantia nigra in Parkinson's disease whereas in progressive supranuclear palsy there was microarchitectural destruction. Elsevier 2016-11-17 /pmc/articles/PMC5144755/ /pubmed/27981030 http://dx.doi.org/10.1016/j.nicl.2016.11.015 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Regular Article
Massey, LA
Miranda, MA
Al-Helli, O
Parkes, HG
Thornton, JS
So, P-W
White, MJ
Mancini, L
Strand, C
Holton, J
Lees, AJ
Revesz, T
Yousry, TA
9.4 T MR microscopy of the substantia nigra with pathological validation in controls and disease
title 9.4 T MR microscopy of the substantia nigra with pathological validation in controls and disease
title_full 9.4 T MR microscopy of the substantia nigra with pathological validation in controls and disease
title_fullStr 9.4 T MR microscopy of the substantia nigra with pathological validation in controls and disease
title_full_unstemmed 9.4 T MR microscopy of the substantia nigra with pathological validation in controls and disease
title_short 9.4 T MR microscopy of the substantia nigra with pathological validation in controls and disease
title_sort 9.4 t mr microscopy of the substantia nigra with pathological validation in controls and disease
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5144755/
https://www.ncbi.nlm.nih.gov/pubmed/27981030
http://dx.doi.org/10.1016/j.nicl.2016.11.015
work_keys_str_mv AT masseyla 94tmrmicroscopyofthesubstantianigrawithpathologicalvalidationincontrolsanddisease
AT mirandama 94tmrmicroscopyofthesubstantianigrawithpathologicalvalidationincontrolsanddisease
AT alhellio 94tmrmicroscopyofthesubstantianigrawithpathologicalvalidationincontrolsanddisease
AT parkeshg 94tmrmicroscopyofthesubstantianigrawithpathologicalvalidationincontrolsanddisease
AT thorntonjs 94tmrmicroscopyofthesubstantianigrawithpathologicalvalidationincontrolsanddisease
AT sopw 94tmrmicroscopyofthesubstantianigrawithpathologicalvalidationincontrolsanddisease
AT whitemj 94tmrmicroscopyofthesubstantianigrawithpathologicalvalidationincontrolsanddisease
AT mancinil 94tmrmicroscopyofthesubstantianigrawithpathologicalvalidationincontrolsanddisease
AT strandc 94tmrmicroscopyofthesubstantianigrawithpathologicalvalidationincontrolsanddisease
AT holtonj 94tmrmicroscopyofthesubstantianigrawithpathologicalvalidationincontrolsanddisease
AT leesaj 94tmrmicroscopyofthesubstantianigrawithpathologicalvalidationincontrolsanddisease
AT reveszt 94tmrmicroscopyofthesubstantianigrawithpathologicalvalidationincontrolsanddisease
AT yousryta 94tmrmicroscopyofthesubstantianigrawithpathologicalvalidationincontrolsanddisease