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Influence of Fasting Status and Sample Preparation on Metabolic Biomarker Measurements in Postmenopausal Women

BACKGROUND: Epidemiologic data linking metabolic markers-such as insulin, insulin-like growth factors (IGFs)-and adipose tissue-derived factors with cancer are inconsistent. Between-study differences in blood collection protocols, in particular participant’s fasting status, may influence measurement...

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Autores principales: Murphy, Neil, Falk, Roni T., Messinger, Diana B., Pollak, Michael, Xue, Xiaonan, Lin, Juan, Sgueglia, Robin, Strickler, Howard D., Gaudet, Mia M., Gunter, Marc J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5145182/
https://www.ncbi.nlm.nih.gov/pubmed/27930694
http://dx.doi.org/10.1371/journal.pone.0167832
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author Murphy, Neil
Falk, Roni T.
Messinger, Diana B.
Pollak, Michael
Xue, Xiaonan
Lin, Juan
Sgueglia, Robin
Strickler, Howard D.
Gaudet, Mia M.
Gunter, Marc J.
author_facet Murphy, Neil
Falk, Roni T.
Messinger, Diana B.
Pollak, Michael
Xue, Xiaonan
Lin, Juan
Sgueglia, Robin
Strickler, Howard D.
Gaudet, Mia M.
Gunter, Marc J.
author_sort Murphy, Neil
collection PubMed
description BACKGROUND: Epidemiologic data linking metabolic markers-such as insulin, insulin-like growth factors (IGFs)-and adipose tissue-derived factors with cancer are inconsistent. Between-study differences in blood collection protocols, in particular participant’s fasting status, may influence measurements. METHODS: We investigated the impact of fasting status and blood sample processing time on components of the insulin/IGF axis and in adipokines in a controlled feeding study of 45 healthy postmenopausal-women aged 50–75 years. Fasting blood samples were drawn (T0), after which subjects ate a standardized breakfast; subsequent blood draws were made at 1 hour (T1), 3 hours (T3), and 6 hours (T6) after breakfast. Serum samples were assayed for insulin, C-peptide, total- and free-IGF-I, IGF-binding protein [BP]-1 and -3, total and high molecular weight (HMW)-adiponectin, retinol binding protein-4, plasminogen activator inhibitor (PAI)-1, and resistin. RESULTS: Insulin and C-peptide levels followed similar postprandial trajectories; intra-class correlation coefficients [ICC] for insulin = 0.75, (95%CI:0.64–0.97) and C-peptide (ICC = 0.66, 95%CI:0.54–0.77) were similarly correlated in fasting (Spearman correlation, r = 0.78, 95%CI:0.64–0.88) and postprandial states (T1, r = 0.77 (95%CI: 0.62–0.87); T3,r = 0.78 (95%CI: 0.63–0.87); T6,r = 0.77 (95%CI: 0.61–0.87)). Free-IGF-I and IGFBP-1 levels were also affected by fasting status, whereas total-IGF-I and IGFBP-3 levels remained unchanged. Levels of adipokines were largely insensitive to fasting status and blood sample processing delays. CONCLUSION: Several components of the insulin/IGF axis were significantly impacted by fasting state and in particular, C-peptide levels were substantially altered postprandially and in a similar manner to insulin.
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spelling pubmed-51451822016-12-22 Influence of Fasting Status and Sample Preparation on Metabolic Biomarker Measurements in Postmenopausal Women Murphy, Neil Falk, Roni T. Messinger, Diana B. Pollak, Michael Xue, Xiaonan Lin, Juan Sgueglia, Robin Strickler, Howard D. Gaudet, Mia M. Gunter, Marc J. PLoS One Research Article BACKGROUND: Epidemiologic data linking metabolic markers-such as insulin, insulin-like growth factors (IGFs)-and adipose tissue-derived factors with cancer are inconsistent. Between-study differences in blood collection protocols, in particular participant’s fasting status, may influence measurements. METHODS: We investigated the impact of fasting status and blood sample processing time on components of the insulin/IGF axis and in adipokines in a controlled feeding study of 45 healthy postmenopausal-women aged 50–75 years. Fasting blood samples were drawn (T0), after which subjects ate a standardized breakfast; subsequent blood draws were made at 1 hour (T1), 3 hours (T3), and 6 hours (T6) after breakfast. Serum samples were assayed for insulin, C-peptide, total- and free-IGF-I, IGF-binding protein [BP]-1 and -3, total and high molecular weight (HMW)-adiponectin, retinol binding protein-4, plasminogen activator inhibitor (PAI)-1, and resistin. RESULTS: Insulin and C-peptide levels followed similar postprandial trajectories; intra-class correlation coefficients [ICC] for insulin = 0.75, (95%CI:0.64–0.97) and C-peptide (ICC = 0.66, 95%CI:0.54–0.77) were similarly correlated in fasting (Spearman correlation, r = 0.78, 95%CI:0.64–0.88) and postprandial states (T1, r = 0.77 (95%CI: 0.62–0.87); T3,r = 0.78 (95%CI: 0.63–0.87); T6,r = 0.77 (95%CI: 0.61–0.87)). Free-IGF-I and IGFBP-1 levels were also affected by fasting status, whereas total-IGF-I and IGFBP-3 levels remained unchanged. Levels of adipokines were largely insensitive to fasting status and blood sample processing delays. CONCLUSION: Several components of the insulin/IGF axis were significantly impacted by fasting state and in particular, C-peptide levels were substantially altered postprandially and in a similar manner to insulin. Public Library of Science 2016-12-08 /pmc/articles/PMC5145182/ /pubmed/27930694 http://dx.doi.org/10.1371/journal.pone.0167832 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Murphy, Neil
Falk, Roni T.
Messinger, Diana B.
Pollak, Michael
Xue, Xiaonan
Lin, Juan
Sgueglia, Robin
Strickler, Howard D.
Gaudet, Mia M.
Gunter, Marc J.
Influence of Fasting Status and Sample Preparation on Metabolic Biomarker Measurements in Postmenopausal Women
title Influence of Fasting Status and Sample Preparation on Metabolic Biomarker Measurements in Postmenopausal Women
title_full Influence of Fasting Status and Sample Preparation on Metabolic Biomarker Measurements in Postmenopausal Women
title_fullStr Influence of Fasting Status and Sample Preparation on Metabolic Biomarker Measurements in Postmenopausal Women
title_full_unstemmed Influence of Fasting Status and Sample Preparation on Metabolic Biomarker Measurements in Postmenopausal Women
title_short Influence of Fasting Status and Sample Preparation on Metabolic Biomarker Measurements in Postmenopausal Women
title_sort influence of fasting status and sample preparation on metabolic biomarker measurements in postmenopausal women
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5145182/
https://www.ncbi.nlm.nih.gov/pubmed/27930694
http://dx.doi.org/10.1371/journal.pone.0167832
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