Cargando…
Nitric oxide-an endogenous inhibitor of gastric acid secretion in isolated human gastric glands
BACKGROUND: Endothelial nitric oxide synthase (eNOS) has previously been detected in the glandular part of the human gastric mucosa. Furthermore, nitric oxide (NO) has been shown to influence gastric secretion in various animal models. The present study was conducted to investigate the influence of...
Autores principales: | , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2004
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC514546/ https://www.ncbi.nlm.nih.gov/pubmed/15298720 http://dx.doi.org/10.1186/1471-230X-4-16 |
_version_ | 1782121724756623360 |
---|---|
author | Berg, Anna Redeen, Stefan Ericson, Ann-Charlott Sjöstrand, Sven Erik |
author_facet | Berg, Anna Redeen, Stefan Ericson, Ann-Charlott Sjöstrand, Sven Erik |
author_sort | Berg, Anna |
collection | PubMed |
description | BACKGROUND: Endothelial nitric oxide synthase (eNOS) has previously been detected in the glandular part of the human gastric mucosa. Furthermore, nitric oxide (NO) has been shown to influence gastric secretion in various animal models. The present study was conducted to investigate the influence of exogenously and endogenously derived NO on histamine- and cAMP-stimulated gastric acid secretion in isolated human oxyntic glands. METHODS: Oxyntic glands were isolated from human gastric biopsies and were subsequently pre-treated with NO donors and nitric oxide synthase inhibitors and then exposed to histamine or dibutyryl-cAMP (db-cAMP). The secretory response of the glands was determined as accumulation of [(14)C]aminopyrine. RESULTS: The histamine- or db-cAMP-induced acid secretion was attenuated by L-arginine, a known source of endogenous NO, and also by the NO-donors sodium nitroprusside (SNP) and S-nitroso-N-acetyl-penicillamine (SNAP). Pre-treatment with either of the NOS inhibitors N(G)-nitro-L-arginine methyl ester (L-NAME) or N(G)-nitro-L-arginine (L-NNA) enhanced the secretory response. CONCLUSION: Our results show that NO inhibits gastric acid secretion in isolated human gastric glands, and that there is endogenous formation of NO within the glandular epithelium in the vicinity of the parietal cells. |
format | Text |
id | pubmed-514546 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-5145462004-08-27 Nitric oxide-an endogenous inhibitor of gastric acid secretion in isolated human gastric glands Berg, Anna Redeen, Stefan Ericson, Ann-Charlott Sjöstrand, Sven Erik BMC Gastroenterol Research Article BACKGROUND: Endothelial nitric oxide synthase (eNOS) has previously been detected in the glandular part of the human gastric mucosa. Furthermore, nitric oxide (NO) has been shown to influence gastric secretion in various animal models. The present study was conducted to investigate the influence of exogenously and endogenously derived NO on histamine- and cAMP-stimulated gastric acid secretion in isolated human oxyntic glands. METHODS: Oxyntic glands were isolated from human gastric biopsies and were subsequently pre-treated with NO donors and nitric oxide synthase inhibitors and then exposed to histamine or dibutyryl-cAMP (db-cAMP). The secretory response of the glands was determined as accumulation of [(14)C]aminopyrine. RESULTS: The histamine- or db-cAMP-induced acid secretion was attenuated by L-arginine, a known source of endogenous NO, and also by the NO-donors sodium nitroprusside (SNP) and S-nitroso-N-acetyl-penicillamine (SNAP). Pre-treatment with either of the NOS inhibitors N(G)-nitro-L-arginine methyl ester (L-NAME) or N(G)-nitro-L-arginine (L-NNA) enhanced the secretory response. CONCLUSION: Our results show that NO inhibits gastric acid secretion in isolated human gastric glands, and that there is endogenous formation of NO within the glandular epithelium in the vicinity of the parietal cells. BioMed Central 2004-08-06 /pmc/articles/PMC514546/ /pubmed/15298720 http://dx.doi.org/10.1186/1471-230X-4-16 Text en Copyright © 2004 Berg et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Berg, Anna Redeen, Stefan Ericson, Ann-Charlott Sjöstrand, Sven Erik Nitric oxide-an endogenous inhibitor of gastric acid secretion in isolated human gastric glands |
title | Nitric oxide-an endogenous inhibitor of gastric acid secretion in isolated human gastric glands |
title_full | Nitric oxide-an endogenous inhibitor of gastric acid secretion in isolated human gastric glands |
title_fullStr | Nitric oxide-an endogenous inhibitor of gastric acid secretion in isolated human gastric glands |
title_full_unstemmed | Nitric oxide-an endogenous inhibitor of gastric acid secretion in isolated human gastric glands |
title_short | Nitric oxide-an endogenous inhibitor of gastric acid secretion in isolated human gastric glands |
title_sort | nitric oxide-an endogenous inhibitor of gastric acid secretion in isolated human gastric glands |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC514546/ https://www.ncbi.nlm.nih.gov/pubmed/15298720 http://dx.doi.org/10.1186/1471-230X-4-16 |
work_keys_str_mv | AT berganna nitricoxideanendogenousinhibitorofgastricacidsecretioninisolatedhumangastricglands AT redeenstefan nitricoxideanendogenousinhibitorofgastricacidsecretioninisolatedhumangastricglands AT ericsonanncharlott nitricoxideanendogenousinhibitorofgastricacidsecretioninisolatedhumangastricglands AT sjostrandsvenerik nitricoxideanendogenousinhibitorofgastricacidsecretioninisolatedhumangastricglands |