Cargando…
Attenuation of dengue virus infection by adeno-associated virus-mediated siRNA delivery
BACKGROUND: The need for safe and effective treatment of dengue virus (DEN), a class A agent that causes dengue hemorrhagic fever/dengue shock syndrome, has been a critical global priority. An effective vaccine for DEN is not yet available. In this study the possibility of attenuating DEN infection...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2004
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC514572/ https://www.ncbi.nlm.nih.gov/pubmed/15301687 http://dx.doi.org/10.1186/1479-0556-2-8 |
_version_ | 1782121730903375872 |
---|---|
author | Zhang, Weidong Singam, Rajeswari Hellermann, Gary Kong, Xiaoyuan Juan, Homero San Lockey, Richard F Wu, Shuen-Ju Porter, Kevin Mohapatra, Shyam S |
author_facet | Zhang, Weidong Singam, Rajeswari Hellermann, Gary Kong, Xiaoyuan Juan, Homero San Lockey, Richard F Wu, Shuen-Ju Porter, Kevin Mohapatra, Shyam S |
author_sort | Zhang, Weidong |
collection | PubMed |
description | BACKGROUND: The need for safe and effective treatment of dengue virus (DEN), a class A agent that causes dengue hemorrhagic fever/dengue shock syndrome, has been a critical global priority. An effective vaccine for DEN is not yet available. In this study the possibility of attenuating DEN infection using adeno-associated virus (AAV)-encoded short interfering RNAs (siRNA) was examined in Vero cells and human dendritic cells (DCs). METHODS: A cassette encoding siRNA targeted to a 3' untranslated sequence common to all DEN serotypes was designed and tested for its ability to attenuate DEN infection by use of AAV delivery. RESULTS: Vero cells or DCs infected with AAV-siRNA showed a significant, dose-dependent reduction in DEN infection. Treatment of DCs with AAV-siRNA also decreased the DEN-induced apoptosis of DCs and did not induce significant inflammation. CONCLUSION: These results demonstrate that AAV-mediated siRNA delivery is capable of reducing DEN infection in cells and may be useful in decreasing DEN replication in humans. |
format | Text |
id | pubmed-514572 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-5145722004-08-27 Attenuation of dengue virus infection by adeno-associated virus-mediated siRNA delivery Zhang, Weidong Singam, Rajeswari Hellermann, Gary Kong, Xiaoyuan Juan, Homero San Lockey, Richard F Wu, Shuen-Ju Porter, Kevin Mohapatra, Shyam S Genet Vaccines Ther Research BACKGROUND: The need for safe and effective treatment of dengue virus (DEN), a class A agent that causes dengue hemorrhagic fever/dengue shock syndrome, has been a critical global priority. An effective vaccine for DEN is not yet available. In this study the possibility of attenuating DEN infection using adeno-associated virus (AAV)-encoded short interfering RNAs (siRNA) was examined in Vero cells and human dendritic cells (DCs). METHODS: A cassette encoding siRNA targeted to a 3' untranslated sequence common to all DEN serotypes was designed and tested for its ability to attenuate DEN infection by use of AAV delivery. RESULTS: Vero cells or DCs infected with AAV-siRNA showed a significant, dose-dependent reduction in DEN infection. Treatment of DCs with AAV-siRNA also decreased the DEN-induced apoptosis of DCs and did not induce significant inflammation. CONCLUSION: These results demonstrate that AAV-mediated siRNA delivery is capable of reducing DEN infection in cells and may be useful in decreasing DEN replication in humans. BioMed Central 2004-08-09 /pmc/articles/PMC514572/ /pubmed/15301687 http://dx.doi.org/10.1186/1479-0556-2-8 Text en Copyright © 2004 Zhang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Zhang, Weidong Singam, Rajeswari Hellermann, Gary Kong, Xiaoyuan Juan, Homero San Lockey, Richard F Wu, Shuen-Ju Porter, Kevin Mohapatra, Shyam S Attenuation of dengue virus infection by adeno-associated virus-mediated siRNA delivery |
title | Attenuation of dengue virus infection by adeno-associated virus-mediated siRNA delivery |
title_full | Attenuation of dengue virus infection by adeno-associated virus-mediated siRNA delivery |
title_fullStr | Attenuation of dengue virus infection by adeno-associated virus-mediated siRNA delivery |
title_full_unstemmed | Attenuation of dengue virus infection by adeno-associated virus-mediated siRNA delivery |
title_short | Attenuation of dengue virus infection by adeno-associated virus-mediated siRNA delivery |
title_sort | attenuation of dengue virus infection by adeno-associated virus-mediated sirna delivery |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC514572/ https://www.ncbi.nlm.nih.gov/pubmed/15301687 http://dx.doi.org/10.1186/1479-0556-2-8 |
work_keys_str_mv | AT zhangweidong attenuationofdenguevirusinfectionbyadenoassociatedvirusmediatedsirnadelivery AT singamrajeswari attenuationofdenguevirusinfectionbyadenoassociatedvirusmediatedsirnadelivery AT hellermanngary attenuationofdenguevirusinfectionbyadenoassociatedvirusmediatedsirnadelivery AT kongxiaoyuan attenuationofdenguevirusinfectionbyadenoassociatedvirusmediatedsirnadelivery AT juanhomerosan attenuationofdenguevirusinfectionbyadenoassociatedvirusmediatedsirnadelivery AT lockeyrichardf attenuationofdenguevirusinfectionbyadenoassociatedvirusmediatedsirnadelivery AT wushuenju attenuationofdenguevirusinfectionbyadenoassociatedvirusmediatedsirnadelivery AT porterkevin attenuationofdenguevirusinfectionbyadenoassociatedvirusmediatedsirnadelivery AT mohapatrashyams attenuationofdenguevirusinfectionbyadenoassociatedvirusmediatedsirnadelivery |