Cargando…

Chronic exposure to indoxacarb and pulmonary expression of toll-like receptor-9 in mice

AIM: Chronic exposure to indoxacarb and pulmonary expression of toll-like receptor 9 (TLR-9) in mice. MATERIALS AND METHODS: In this study, healthy male Swiss albino mice (n=30) aging 8-10 weeks were used to evaluate TLR-9 expression in lungs of mice following indoxacarb exposure with and without li...

Descripción completa

Detalles Bibliográficos
Autores principales: Kaur, Sandeep, Mukhopadhyay, C. S., Sethi, R. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5146311/
https://www.ncbi.nlm.nih.gov/pubmed/27956782
http://dx.doi.org/10.14202/vetworld.2016.1282-1286
_version_ 1782473460283342848
author Kaur, Sandeep
Mukhopadhyay, C. S.
Sethi, R. S.
author_facet Kaur, Sandeep
Mukhopadhyay, C. S.
Sethi, R. S.
author_sort Kaur, Sandeep
collection PubMed
description AIM: Chronic exposure to indoxacarb and pulmonary expression of toll-like receptor 9 (TLR-9) in mice. MATERIALS AND METHODS: In this study, healthy male Swiss albino mice (n=30) aging 8-10 weeks were used to evaluate TLR-9 expression in lungs of mice following indoxacarb exposure with and without lipopolysaccharide (LPS). Indoxacarb was administered orally dissolved in groundnut oil at 4 and 2 mg/kg/day for 90 days. On day 91, five animals from each group were challenged with LPS/normal saline solution at 80 µg/animal. The lung tissues were processed for real time and immunohistochemical studies. RESULTS: LPS resulted increase in fold change m-RNA expression level of TLR-9 as compare to control, while indoxacarb (4 mg/kg) alone and in combination with LPS resulted 16.21-fold change and 29.4-fold change increase in expression of TLR-9 m-RNA, respectively, as compared to control. Similarly, indoxacarb (2 mg/kg) alone or in combination with LPS also altered TLR-9 expression. Further at protein level control group showed minimal expression of TLR-9 in lungs as compare to other groups, however, LPS group showed intense positive staining in bronchial epithelium as well as in alveolar septal cells. Indoxacarb at both doses individually showed strong immuno-positive reaction as compare to control, however when combined with LPS resulted intense staining in airway epithelium as compare to control. CONCLUSION: Chronic oral administration of indoxacarb for 90 days (4 and 2 mg/kg) alters expression of TLR-9 at m-RNA and protein level and co-exposure with LPS exhibited synergistic effect.
format Online
Article
Text
id pubmed-5146311
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Veterinary World
record_format MEDLINE/PubMed
spelling pubmed-51463112016-12-12 Chronic exposure to indoxacarb and pulmonary expression of toll-like receptor-9 in mice Kaur, Sandeep Mukhopadhyay, C. S. Sethi, R. S. Vet World Research Article AIM: Chronic exposure to indoxacarb and pulmonary expression of toll-like receptor 9 (TLR-9) in mice. MATERIALS AND METHODS: In this study, healthy male Swiss albino mice (n=30) aging 8-10 weeks were used to evaluate TLR-9 expression in lungs of mice following indoxacarb exposure with and without lipopolysaccharide (LPS). Indoxacarb was administered orally dissolved in groundnut oil at 4 and 2 mg/kg/day for 90 days. On day 91, five animals from each group were challenged with LPS/normal saline solution at 80 µg/animal. The lung tissues were processed for real time and immunohistochemical studies. RESULTS: LPS resulted increase in fold change m-RNA expression level of TLR-9 as compare to control, while indoxacarb (4 mg/kg) alone and in combination with LPS resulted 16.21-fold change and 29.4-fold change increase in expression of TLR-9 m-RNA, respectively, as compared to control. Similarly, indoxacarb (2 mg/kg) alone or in combination with LPS also altered TLR-9 expression. Further at protein level control group showed minimal expression of TLR-9 in lungs as compare to other groups, however, LPS group showed intense positive staining in bronchial epithelium as well as in alveolar septal cells. Indoxacarb at both doses individually showed strong immuno-positive reaction as compare to control, however when combined with LPS resulted intense staining in airway epithelium as compare to control. CONCLUSION: Chronic oral administration of indoxacarb for 90 days (4 and 2 mg/kg) alters expression of TLR-9 at m-RNA and protein level and co-exposure with LPS exhibited synergistic effect. Veterinary World 2016-11 2016-11-21 /pmc/articles/PMC5146311/ /pubmed/27956782 http://dx.doi.org/10.14202/vetworld.2016.1282-1286 Text en Copyright: © Kaur, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Kaur, Sandeep
Mukhopadhyay, C. S.
Sethi, R. S.
Chronic exposure to indoxacarb and pulmonary expression of toll-like receptor-9 in mice
title Chronic exposure to indoxacarb and pulmonary expression of toll-like receptor-9 in mice
title_full Chronic exposure to indoxacarb and pulmonary expression of toll-like receptor-9 in mice
title_fullStr Chronic exposure to indoxacarb and pulmonary expression of toll-like receptor-9 in mice
title_full_unstemmed Chronic exposure to indoxacarb and pulmonary expression of toll-like receptor-9 in mice
title_short Chronic exposure to indoxacarb and pulmonary expression of toll-like receptor-9 in mice
title_sort chronic exposure to indoxacarb and pulmonary expression of toll-like receptor-9 in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5146311/
https://www.ncbi.nlm.nih.gov/pubmed/27956782
http://dx.doi.org/10.14202/vetworld.2016.1282-1286
work_keys_str_mv AT kaursandeep chronicexposuretoindoxacarbandpulmonaryexpressionoftolllikereceptor9inmice
AT mukhopadhyaycs chronicexposuretoindoxacarbandpulmonaryexpressionoftolllikereceptor9inmice
AT sethirs chronicexposuretoindoxacarbandpulmonaryexpressionoftolllikereceptor9inmice