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Usage of Murine T-cell Hybridoma Cells as Responder Cells Reveals Interference of Helicobacter Pylori with Human Dendritic Cell-mediated Antigen Presentation

Direct effects of Helicobacter pylori (H. pylori) on human CD4(+) T-cells hamper disentangling a possible bacterial-mediated interference with major histocompatibility complex class II (MHC-II)-dependent antigen presentation to these cells. To overcome this limitation, we employed a previously descr...

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Detalles Bibliográficos
Autores principales: Fehlings, Michael, Drobbe, Lea, Beigier-Bompadre, Macarena, Viveros, Pablo Renner, Moos, Verena, Schneider, Thomas, Meyer, Thomas F., Aebischer, Toni, Ignatius, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Akadémiai Kiadó 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5146649/
https://www.ncbi.nlm.nih.gov/pubmed/27980859
http://dx.doi.org/10.1556/1886.2016.00031
Descripción
Sumario:Direct effects of Helicobacter pylori (H. pylori) on human CD4(+) T-cells hamper disentangling a possible bacterial-mediated interference with major histocompatibility complex class II (MHC-II)-dependent antigen presentation to these cells. To overcome this limitation, we employed a previously described assay, which enables assessing human antigen-processing cell function by using murine T-cell hybridoma cells restricted by human leukocyte antigen (HLA) alleles. HLA-DR1(+) monocyte-derived dendritic cells were exposed to H. pylori and pulsed with the antigen 85B from Mycobacterium tuberculosis (M. tuberculosis). Interleukin-2 (IL-2) secretion by AG85B(aa97-112)-specific hybridoma cells was then evaluated as an integral reporter of cognate antigen presentation. This methodology enabled revealing of interference of H. pylori with the antigen-presenting capacity of human dendritic cells.