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Comparison of the antimicrobial activity of direct pulp-capping materials: Mineral trioxide aggregate-Angelus and Biodentine

PURPOSE: To compare the antimicrobial activity of the tricalcium silicate-based Biodentine (BD) and mineral trioxide aggregate (MTA)-Angelus cement with the aid of agar diffusion test. MATERIALS AND METHODS: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Enterococcus faecium we...

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Autores principales: Özyürek, Taha, Demiryürek, Ebru Özsezer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5146775/
https://www.ncbi.nlm.nih.gov/pubmed/27994321
http://dx.doi.org/10.4103/0972-0707.194018
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author Özyürek, Taha
Demiryürek, Ebru Özsezer
author_facet Özyürek, Taha
Demiryürek, Ebru Özsezer
author_sort Özyürek, Taha
collection PubMed
description PURPOSE: To compare the antimicrobial activity of the tricalcium silicate-based Biodentine (BD) and mineral trioxide aggregate (MTA)-Angelus cement with the aid of agar diffusion test. MATERIALS AND METHODS: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Enterococcus faecium were inoculated in the Brucella liquid medium and were incubated at 37°C for 24 h. Thereafter, 100 >μl of the liquid culture of bacteria inoculated in the Mueller-Hinton agar with spread plate technique. Petri plates were dried in room temperature. For every microorganism, 3 petri plates were prepared (12 in total). In the medium, in every petri plate, 2 holes with 5 mm diameter and 2 mm depth were made. Afterward, BD and MTA-Angelus were filled into these holes under aseptic conditions according to the instructions of the manufacturing company. Then, the plates were kept in the incubator at 37°C for 24 h, and the diameters of the inhibition zones were measured with a digital caliper. RESULTS: Inhibition zones formed by BD against E. coli and S. aureus were significantly larger than the zones formed by MTA-Angelus (P < 0.05). However, the inhibition zones formed by MTA-Angelus against P. aureus and E. faecium were larger than the zones formed by BD (P < 0.05). CONCLUSION: Within the limits of the present study, tricalcium silicate-based MTA-Angelus and BD have antimicrobial activity against E. coli, S. aureus, P. aureus, and E. faecium.
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spelling pubmed-51467752016-12-19 Comparison of the antimicrobial activity of direct pulp-capping materials: Mineral trioxide aggregate-Angelus and Biodentine Özyürek, Taha Demiryürek, Ebru Özsezer J Conserv Dent Original Research Article PURPOSE: To compare the antimicrobial activity of the tricalcium silicate-based Biodentine (BD) and mineral trioxide aggregate (MTA)-Angelus cement with the aid of agar diffusion test. MATERIALS AND METHODS: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Enterococcus faecium were inoculated in the Brucella liquid medium and were incubated at 37°C for 24 h. Thereafter, 100 >μl of the liquid culture of bacteria inoculated in the Mueller-Hinton agar with spread plate technique. Petri plates were dried in room temperature. For every microorganism, 3 petri plates were prepared (12 in total). In the medium, in every petri plate, 2 holes with 5 mm diameter and 2 mm depth were made. Afterward, BD and MTA-Angelus were filled into these holes under aseptic conditions according to the instructions of the manufacturing company. Then, the plates were kept in the incubator at 37°C for 24 h, and the diameters of the inhibition zones were measured with a digital caliper. RESULTS: Inhibition zones formed by BD against E. coli and S. aureus were significantly larger than the zones formed by MTA-Angelus (P < 0.05). However, the inhibition zones formed by MTA-Angelus against P. aureus and E. faecium were larger than the zones formed by BD (P < 0.05). CONCLUSION: Within the limits of the present study, tricalcium silicate-based MTA-Angelus and BD have antimicrobial activity against E. coli, S. aureus, P. aureus, and E. faecium. Medknow Publications & Media Pvt Ltd 2016 /pmc/articles/PMC5146775/ /pubmed/27994321 http://dx.doi.org/10.4103/0972-0707.194018 Text en Copyright: © Journal of Conservative Dentistry http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Research Article
Özyürek, Taha
Demiryürek, Ebru Özsezer
Comparison of the antimicrobial activity of direct pulp-capping materials: Mineral trioxide aggregate-Angelus and Biodentine
title Comparison of the antimicrobial activity of direct pulp-capping materials: Mineral trioxide aggregate-Angelus and Biodentine
title_full Comparison of the antimicrobial activity of direct pulp-capping materials: Mineral trioxide aggregate-Angelus and Biodentine
title_fullStr Comparison of the antimicrobial activity of direct pulp-capping materials: Mineral trioxide aggregate-Angelus and Biodentine
title_full_unstemmed Comparison of the antimicrobial activity of direct pulp-capping materials: Mineral trioxide aggregate-Angelus and Biodentine
title_short Comparison of the antimicrobial activity of direct pulp-capping materials: Mineral trioxide aggregate-Angelus and Biodentine
title_sort comparison of the antimicrobial activity of direct pulp-capping materials: mineral trioxide aggregate-angelus and biodentine
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5146775/
https://www.ncbi.nlm.nih.gov/pubmed/27994321
http://dx.doi.org/10.4103/0972-0707.194018
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