Cargando…

Evaluating Anti-SmD1-amino-acid 83-119 Peptide Reactivity in Children with Systemic Lupus Erythematosus and Other Immunological Diseases

BACKGROUND: SmD1-amino-acid 83-119 peptide (SmD1(83-119)) is the major epitope of Smith (Sm) antigen, which is specific for adult systemic lupus erythematosus (SLE). The anti-SmD1(83-119) antibody has exhibited higher sensitivity and specificity than anti-Sm antibody in diagnosing adult SLE. However...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Hai-Ou, Zhang, Xiao-Qing, Fu, Qi-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5146793/
https://www.ncbi.nlm.nih.gov/pubmed/27900999
http://dx.doi.org/10.4103/0366-6999.194653
_version_ 1782473551773696000
author Yang, Hai-Ou
Zhang, Xiao-Qing
Fu, Qi-Hua
author_facet Yang, Hai-Ou
Zhang, Xiao-Qing
Fu, Qi-Hua
author_sort Yang, Hai-Ou
collection PubMed
description BACKGROUND: SmD1-amino-acid 83-119 peptide (SmD1(83-119)) is the major epitope of Smith (Sm) antigen, which is specific for adult systemic lupus erythematosus (SLE). The anti-SmD1(83-119) antibody has exhibited higher sensitivity and specificity than anti-Sm antibody in diagnosing adult SLE. However, the utility of anti-SmD1(83-119) antibodies remains unclear in children with SLE (cSLE). This study aimed to assess the characteristics of anti-SmD1(83-119) antibody in the diagnosis of cSLE. METHODS: Samples from 242 children with different rheumatological and immunological disorders, including autoimmune diseases (SLE [n = 46] and ankylosing spondylitis [AS, n = 11]), nonautoimmune diseases (Henoch-Schonlein purpura [HSP, n = 60], idiopathic thrombocytopenia purpura [n = 27], hematuria [n = 59], and arthralgia [n = 39]) were collected from Shanghai Children's Medical Center from March 6, 2012 to February 27, 2014. Seventy age- and sex-matched patients were enrolled in this study as the negative controls. All the patients' sera were analyzed for the anti-SmD1(83-119), anti-Sm, anti-U1-nRNP, anti-double-stranded DNA (dsDNA), anti-nucleosome, anti-SSA/Ro60, anti-SSA/Ro52, anti-SSB, anti-Scl-70, and anti-histone antibodies using the immunoblotting assay. The differences in sensitivity and specificity between anti-SmD1(83-119) and anti-Sm antibodies were compared by Chi-square test. The correlations between anti-SmD1(83-119) and other auto-antibodies were analyzed using the Spearman's correlation analysis. A value of P < 0.05 was considered statistically significant. RESULTS: Thirty-six out of 46 patients with cSLE were found to be positive for anti-SmD1(83-119), while 12 patients from the cSLE cohort were found to be positive for anti-Sm. Compared to cSLE, it has been shown that anti-SmD1(83-119) was only detected in 27.3% of patients with AS and 16.7% of patients with HSP. In comparison with anti-Sm, it has been demonstrated that anti-SmD1(83-119) had a higher sensitivity (78.3% vs. 26.1%, χ(2) = 25.1, P < 0.05) and a lower specificity (90.8% vs. 100%, χ(2) = 13.6, P < 0.05) in the diagnosis of cSLE. Further analysis revealed that anti-SmD1(83-119) antibodies were positively correlated with anti-dsDNA, anti-nucleosome, and anti-histone antibodies in cSLE. Moreover, it has been clearly shown that anti-SmD1(83-119) was more sensitive than anti-Sm in discriminating autoimmune diseases from nonautoimmune disorders in patients with arthralgia or hematuria. CONCLUSIONS: Measurement of anti-SmD1(83-119) in patients with cSLE has a higher sensitivity and a marginally lower specificity than anti-Sm. It has been suggested that inclusion of anti-SmD1(83-119) testing in the integrated laboratory diagnosis of cSLE may significantly improve the overall sensitivity in child populations.
format Online
Article
Text
id pubmed-5146793
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-51467932016-12-19 Evaluating Anti-SmD1-amino-acid 83-119 Peptide Reactivity in Children with Systemic Lupus Erythematosus and Other Immunological Diseases Yang, Hai-Ou Zhang, Xiao-Qing Fu, Qi-Hua Chin Med J (Engl) Original Article BACKGROUND: SmD1-amino-acid 83-119 peptide (SmD1(83-119)) is the major epitope of Smith (Sm) antigen, which is specific for adult systemic lupus erythematosus (SLE). The anti-SmD1(83-119) antibody has exhibited higher sensitivity and specificity than anti-Sm antibody in diagnosing adult SLE. However, the utility of anti-SmD1(83-119) antibodies remains unclear in children with SLE (cSLE). This study aimed to assess the characteristics of anti-SmD1(83-119) antibody in the diagnosis of cSLE. METHODS: Samples from 242 children with different rheumatological and immunological disorders, including autoimmune diseases (SLE [n = 46] and ankylosing spondylitis [AS, n = 11]), nonautoimmune diseases (Henoch-Schonlein purpura [HSP, n = 60], idiopathic thrombocytopenia purpura [n = 27], hematuria [n = 59], and arthralgia [n = 39]) were collected from Shanghai Children's Medical Center from March 6, 2012 to February 27, 2014. Seventy age- and sex-matched patients were enrolled in this study as the negative controls. All the patients' sera were analyzed for the anti-SmD1(83-119), anti-Sm, anti-U1-nRNP, anti-double-stranded DNA (dsDNA), anti-nucleosome, anti-SSA/Ro60, anti-SSA/Ro52, anti-SSB, anti-Scl-70, and anti-histone antibodies using the immunoblotting assay. The differences in sensitivity and specificity between anti-SmD1(83-119) and anti-Sm antibodies were compared by Chi-square test. The correlations between anti-SmD1(83-119) and other auto-antibodies were analyzed using the Spearman's correlation analysis. A value of P < 0.05 was considered statistically significant. RESULTS: Thirty-six out of 46 patients with cSLE were found to be positive for anti-SmD1(83-119), while 12 patients from the cSLE cohort were found to be positive for anti-Sm. Compared to cSLE, it has been shown that anti-SmD1(83-119) was only detected in 27.3% of patients with AS and 16.7% of patients with HSP. In comparison with anti-Sm, it has been demonstrated that anti-SmD1(83-119) had a higher sensitivity (78.3% vs. 26.1%, χ(2) = 25.1, P < 0.05) and a lower specificity (90.8% vs. 100%, χ(2) = 13.6, P < 0.05) in the diagnosis of cSLE. Further analysis revealed that anti-SmD1(83-119) antibodies were positively correlated with anti-dsDNA, anti-nucleosome, and anti-histone antibodies in cSLE. Moreover, it has been clearly shown that anti-SmD1(83-119) was more sensitive than anti-Sm in discriminating autoimmune diseases from nonautoimmune disorders in patients with arthralgia or hematuria. CONCLUSIONS: Measurement of anti-SmD1(83-119) in patients with cSLE has a higher sensitivity and a marginally lower specificity than anti-Sm. It has been suggested that inclusion of anti-SmD1(83-119) testing in the integrated laboratory diagnosis of cSLE may significantly improve the overall sensitivity in child populations. Medknow Publications & Media Pvt Ltd 2016-12-05 /pmc/articles/PMC5146793/ /pubmed/27900999 http://dx.doi.org/10.4103/0366-6999.194653 Text en Copyright: © 2016 Chinese Medical Journal http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Yang, Hai-Ou
Zhang, Xiao-Qing
Fu, Qi-Hua
Evaluating Anti-SmD1-amino-acid 83-119 Peptide Reactivity in Children with Systemic Lupus Erythematosus and Other Immunological Diseases
title Evaluating Anti-SmD1-amino-acid 83-119 Peptide Reactivity in Children with Systemic Lupus Erythematosus and Other Immunological Diseases
title_full Evaluating Anti-SmD1-amino-acid 83-119 Peptide Reactivity in Children with Systemic Lupus Erythematosus and Other Immunological Diseases
title_fullStr Evaluating Anti-SmD1-amino-acid 83-119 Peptide Reactivity in Children with Systemic Lupus Erythematosus and Other Immunological Diseases
title_full_unstemmed Evaluating Anti-SmD1-amino-acid 83-119 Peptide Reactivity in Children with Systemic Lupus Erythematosus and Other Immunological Diseases
title_short Evaluating Anti-SmD1-amino-acid 83-119 Peptide Reactivity in Children with Systemic Lupus Erythematosus and Other Immunological Diseases
title_sort evaluating anti-smd1-amino-acid 83-119 peptide reactivity in children with systemic lupus erythematosus and other immunological diseases
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5146793/
https://www.ncbi.nlm.nih.gov/pubmed/27900999
http://dx.doi.org/10.4103/0366-6999.194653
work_keys_str_mv AT yanghaiou evaluatingantismd1aminoacid83119peptidereactivityinchildrenwithsystemiclupuserythematosusandotherimmunologicaldiseases
AT zhangxiaoqing evaluatingantismd1aminoacid83119peptidereactivityinchildrenwithsystemiclupuserythematosusandotherimmunologicaldiseases
AT fuqihua evaluatingantismd1aminoacid83119peptidereactivityinchildrenwithsystemiclupuserythematosusandotherimmunologicaldiseases