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Scutellaria barbata flavonoids alleviate memory deficits and neuronal injuries induced by composited Aβ in rats

BACKGROUND: The aim of the present study was to investigate the effects of Scutellaria barbata flavonoids (SBF) on memory impairment and neuronal injury induced by amyloid beta protein 25–35 in combination with aluminum trichloride (AlCl3) and recombinant human transforming growth factor-β1 (RHTGF-β...

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Autores principales: Wu, Xiao G., Wang, Shu S., Miao, Hong, Cheng, Jian J., Zhang, Shu F., Shang, Ya Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5146811/
https://www.ncbi.nlm.nih.gov/pubmed/27931218
http://dx.doi.org/10.1186/s12993-016-0118-8
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author Wu, Xiao G.
Wang, Shu S.
Miao, Hong
Cheng, Jian J.
Zhang, Shu F.
Shang, Ya Z.
author_facet Wu, Xiao G.
Wang, Shu S.
Miao, Hong
Cheng, Jian J.
Zhang, Shu F.
Shang, Ya Z.
author_sort Wu, Xiao G.
collection PubMed
description BACKGROUND: The aim of the present study was to investigate the effects of Scutellaria barbata flavonoids (SBF) on memory impairment and neuronal injury induced by amyloid beta protein 25–35 in combination with aluminum trichloride (AlCl3) and recombinant human transforming growth factor-β1 (RHTGF-β1) (composited Aβ) in rats. METHODS: The composited Aβ-treated model of Alzheimer’s disease (AD)-like memory impairment and neuronal injury was established in male rats by right intracerebroventricular injection of composited Aβ, and the effects of SBF were assessed using this rat model. Spatial learning and memory of rats were assessed in the Morris water maze, and neuronal injury was assessed by light and electron microscopy with hematoxylin-eosin or uranyl acetate and lead nitrate-sodium citrate staining, respectively. RESULTS: In the Morris water maze, memory impairment was observed in 94.7% of the composited Aβ-treated rats. The composited Aβ-treated rats took longer than sham-operated rats to find the hidden platform during position navigation and reversal learning trials. They also spent less time swimming in the target quadrant in the probe trial. Optical and electron microscopic observations showed significant neuropathological changes including neuron loss or pyknosis in hippocampus, typical colliquative necrosis in cerebral cortex, mitochondrial swelling and cristae fragmentation and a large number of lipofuscin deposits in the cytoplasm. Treatment with SBF (35–140 mg/kg) reduced the memory impairment and neuronal injury induced by composited Aβ. CONCLUSION: SBF-mediated improvement of composited Aβ-induced memory impairment and neuronal injury in rats provides an appropriate rationale for evaluating SBF as a promising agent for treatment of AD. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12993-016-0118-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-51468112016-12-15 Scutellaria barbata flavonoids alleviate memory deficits and neuronal injuries induced by composited Aβ in rats Wu, Xiao G. Wang, Shu S. Miao, Hong Cheng, Jian J. Zhang, Shu F. Shang, Ya Z. Behav Brain Funct Research BACKGROUND: The aim of the present study was to investigate the effects of Scutellaria barbata flavonoids (SBF) on memory impairment and neuronal injury induced by amyloid beta protein 25–35 in combination with aluminum trichloride (AlCl3) and recombinant human transforming growth factor-β1 (RHTGF-β1) (composited Aβ) in rats. METHODS: The composited Aβ-treated model of Alzheimer’s disease (AD)-like memory impairment and neuronal injury was established in male rats by right intracerebroventricular injection of composited Aβ, and the effects of SBF were assessed using this rat model. Spatial learning and memory of rats were assessed in the Morris water maze, and neuronal injury was assessed by light and electron microscopy with hematoxylin-eosin or uranyl acetate and lead nitrate-sodium citrate staining, respectively. RESULTS: In the Morris water maze, memory impairment was observed in 94.7% of the composited Aβ-treated rats. The composited Aβ-treated rats took longer than sham-operated rats to find the hidden platform during position navigation and reversal learning trials. They also spent less time swimming in the target quadrant in the probe trial. Optical and electron microscopic observations showed significant neuropathological changes including neuron loss or pyknosis in hippocampus, typical colliquative necrosis in cerebral cortex, mitochondrial swelling and cristae fragmentation and a large number of lipofuscin deposits in the cytoplasm. Treatment with SBF (35–140 mg/kg) reduced the memory impairment and neuronal injury induced by composited Aβ. CONCLUSION: SBF-mediated improvement of composited Aβ-induced memory impairment and neuronal injury in rats provides an appropriate rationale for evaluating SBF as a promising agent for treatment of AD. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12993-016-0118-8) contains supplementary material, which is available to authorized users. BioMed Central 2016-12-08 /pmc/articles/PMC5146811/ /pubmed/27931218 http://dx.doi.org/10.1186/s12993-016-0118-8 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Wu, Xiao G.
Wang, Shu S.
Miao, Hong
Cheng, Jian J.
Zhang, Shu F.
Shang, Ya Z.
Scutellaria barbata flavonoids alleviate memory deficits and neuronal injuries induced by composited Aβ in rats
title Scutellaria barbata flavonoids alleviate memory deficits and neuronal injuries induced by composited Aβ in rats
title_full Scutellaria barbata flavonoids alleviate memory deficits and neuronal injuries induced by composited Aβ in rats
title_fullStr Scutellaria barbata flavonoids alleviate memory deficits and neuronal injuries induced by composited Aβ in rats
title_full_unstemmed Scutellaria barbata flavonoids alleviate memory deficits and neuronal injuries induced by composited Aβ in rats
title_short Scutellaria barbata flavonoids alleviate memory deficits and neuronal injuries induced by composited Aβ in rats
title_sort scutellaria barbata flavonoids alleviate memory deficits and neuronal injuries induced by composited aβ in rats
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5146811/
https://www.ncbi.nlm.nih.gov/pubmed/27931218
http://dx.doi.org/10.1186/s12993-016-0118-8
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