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Characteristics of a thyroid carcinoma cell line derived from spinal metastasis

A thyroid carcinoma cell line named THY28 was established through primary culture of the surgical specimens, which were derived from a Chinese patient with spinal metastasis. The cell morphology, growth kinetics, cell cycle, chromosome number, cell capability of migration, tumorigenicity and cytogen...

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Detalles Bibliográficos
Autores principales: Zhou, Zhenhua, Li, Yan, Yan, Xu, Wang, Xudong, Chen, Su, Xiao, Jianru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5146815/
https://www.ncbi.nlm.nih.gov/pubmed/27811013
http://dx.doi.org/10.1042/BSR20160403
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author Zhou, Zhenhua
Li, Yan
Yan, Xu
Wang, Xudong
Chen, Su
Xiao, Jianru
author_facet Zhou, Zhenhua
Li, Yan
Yan, Xu
Wang, Xudong
Chen, Su
Xiao, Jianru
author_sort Zhou, Zhenhua
collection PubMed
description A thyroid carcinoma cell line named THY28 was established through primary culture of the surgical specimens, which were derived from a Chinese patient with spinal metastasis. The cell morphology, growth kinetics, cell cycle, chromosome number, cell capability of migration, tumorigenicity and cytogenetic features of the cell line were investigated. THY28 cells were subcultured in vitro for more than 50 passages with a human karyotype. The modal number of its chromosomes was mainly from 67 to 85. The doubling time of THY28 cells was 56 hours. The histopathological features of xenograft induced by THY28 cells were consistent with the characteristics of thyroid cancer. The biological and molecular properties of THY28 cells were not entirely consistent with those of other thyroid carcinoma cells such as SW579 and TT cells, indicating biological differences between primary and metastatic thyroid carcinoma cell lines. We have established a novel thyroid carcinoma cell line derived from spinal metastasis, which will provide a useful model for biological or therapeutic studies of thyroid carcinoma metastasis.
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spelling pubmed-51468152016-12-22 Characteristics of a thyroid carcinoma cell line derived from spinal metastasis Zhou, Zhenhua Li, Yan Yan, Xu Wang, Xudong Chen, Su Xiao, Jianru Biosci Rep Original Papers A thyroid carcinoma cell line named THY28 was established through primary culture of the surgical specimens, which were derived from a Chinese patient with spinal metastasis. The cell morphology, growth kinetics, cell cycle, chromosome number, cell capability of migration, tumorigenicity and cytogenetic features of the cell line were investigated. THY28 cells were subcultured in vitro for more than 50 passages with a human karyotype. The modal number of its chromosomes was mainly from 67 to 85. The doubling time of THY28 cells was 56 hours. The histopathological features of xenograft induced by THY28 cells were consistent with the characteristics of thyroid cancer. The biological and molecular properties of THY28 cells were not entirely consistent with those of other thyroid carcinoma cells such as SW579 and TT cells, indicating biological differences between primary and metastatic thyroid carcinoma cell lines. We have established a novel thyroid carcinoma cell line derived from spinal metastasis, which will provide a useful model for biological or therapeutic studies of thyroid carcinoma metastasis. Portland Press Ltd. 2016-12-09 /pmc/articles/PMC5146815/ /pubmed/27811013 http://dx.doi.org/10.1042/BSR20160403 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Papers
Zhou, Zhenhua
Li, Yan
Yan, Xu
Wang, Xudong
Chen, Su
Xiao, Jianru
Characteristics of a thyroid carcinoma cell line derived from spinal metastasis
title Characteristics of a thyroid carcinoma cell line derived from spinal metastasis
title_full Characteristics of a thyroid carcinoma cell line derived from spinal metastasis
title_fullStr Characteristics of a thyroid carcinoma cell line derived from spinal metastasis
title_full_unstemmed Characteristics of a thyroid carcinoma cell line derived from spinal metastasis
title_short Characteristics of a thyroid carcinoma cell line derived from spinal metastasis
title_sort characteristics of a thyroid carcinoma cell line derived from spinal metastasis
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5146815/
https://www.ncbi.nlm.nih.gov/pubmed/27811013
http://dx.doi.org/10.1042/BSR20160403
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