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miR-455 inhibits neuronal cell death by targeting TRAF3 in cerebral ischemic stroke
Ischemic stroke is one of the leading causes of brain disease, with high morbidity, disability, and mortality. MicroRNAs (miRNAs) have been identified as vital gene regulators in various types of human diseases. Accumulating evidence has suggested that aberrant expression of miRNAs play critical rol...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5147416/ https://www.ncbi.nlm.nih.gov/pubmed/27980410 http://dx.doi.org/10.2147/NDT.S121183 |
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author | Yao, Shengtao Tang, Bo Li, Gang Fan, Ruiming Cao, Fang |
author_facet | Yao, Shengtao Tang, Bo Li, Gang Fan, Ruiming Cao, Fang |
author_sort | Yao, Shengtao |
collection | PubMed |
description | Ischemic stroke is one of the leading causes of brain disease, with high morbidity, disability, and mortality. MicroRNAs (miRNAs) have been identified as vital gene regulators in various types of human diseases. Accumulating evidence has suggested that aberrant expression of miRNAs play critical roles in the pathologies of ischemic stroke. Yet, the precise mechanism by which miRNAs control cerebral ischemic stroke remains unclear. In the present study, we explored whether miR-455 suppresses neuronal death by targeting TRAF3 in cerebral ischemic stroke. The expression levels of miR-455 and TRAF3 were detected by quantitative real-time polymerase chain reaction and Western blot. The role of miR-455 in cell death caused by oxygen–glucose deprivation (OGD) was assessed using Cell Counting Kit-8 (CCK-8) assay. The influence of miR-455 on infarct volume was evaluated in mouse brain after middle cerebral artery occlusion (MCAO). Bioinformatics softwares and luciferase analysis were used to find and confirm the targets of miR-455. The results showed that the expression levels of miR-455 significantly decreased in primary neuronal cells subjected to OGD and mouse brain subjected to MCAO. In addition, forced expression of miR-455 inhibited neuronal death and weakened ischemic brain infarction in focal ischemia-stroked mice. Furthermore, TRAF3 was proved to be a direct target of miR-455, and miR-455 could negatively suppress TRAF3 expression. Biological function analysis showed that TRAF3 silencing displayed the neuroprotective effect in ischemic stroke and could enhance miR-455-induced positive impact on ischemic injury both in vitro and in vivo. Taken together, miR-455 played a vital role in protecting neuronal cells from death by downregulating TRAF3 protein expression. These findings may represent a novel latent therapeutic target for cerebral ischemic stroke. |
format | Online Article Text |
id | pubmed-5147416 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-51474162016-12-15 miR-455 inhibits neuronal cell death by targeting TRAF3 in cerebral ischemic stroke Yao, Shengtao Tang, Bo Li, Gang Fan, Ruiming Cao, Fang Neuropsychiatr Dis Treat Original Research Ischemic stroke is one of the leading causes of brain disease, with high morbidity, disability, and mortality. MicroRNAs (miRNAs) have been identified as vital gene regulators in various types of human diseases. Accumulating evidence has suggested that aberrant expression of miRNAs play critical roles in the pathologies of ischemic stroke. Yet, the precise mechanism by which miRNAs control cerebral ischemic stroke remains unclear. In the present study, we explored whether miR-455 suppresses neuronal death by targeting TRAF3 in cerebral ischemic stroke. The expression levels of miR-455 and TRAF3 were detected by quantitative real-time polymerase chain reaction and Western blot. The role of miR-455 in cell death caused by oxygen–glucose deprivation (OGD) was assessed using Cell Counting Kit-8 (CCK-8) assay. The influence of miR-455 on infarct volume was evaluated in mouse brain after middle cerebral artery occlusion (MCAO). Bioinformatics softwares and luciferase analysis were used to find and confirm the targets of miR-455. The results showed that the expression levels of miR-455 significantly decreased in primary neuronal cells subjected to OGD and mouse brain subjected to MCAO. In addition, forced expression of miR-455 inhibited neuronal death and weakened ischemic brain infarction in focal ischemia-stroked mice. Furthermore, TRAF3 was proved to be a direct target of miR-455, and miR-455 could negatively suppress TRAF3 expression. Biological function analysis showed that TRAF3 silencing displayed the neuroprotective effect in ischemic stroke and could enhance miR-455-induced positive impact on ischemic injury both in vitro and in vivo. Taken together, miR-455 played a vital role in protecting neuronal cells from death by downregulating TRAF3 protein expression. These findings may represent a novel latent therapeutic target for cerebral ischemic stroke. Dove Medical Press 2016-12-05 /pmc/articles/PMC5147416/ /pubmed/27980410 http://dx.doi.org/10.2147/NDT.S121183 Text en © 2016 Yao et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Yao, Shengtao Tang, Bo Li, Gang Fan, Ruiming Cao, Fang miR-455 inhibits neuronal cell death by targeting TRAF3 in cerebral ischemic stroke |
title | miR-455 inhibits neuronal cell death by targeting TRAF3 in cerebral ischemic stroke |
title_full | miR-455 inhibits neuronal cell death by targeting TRAF3 in cerebral ischemic stroke |
title_fullStr | miR-455 inhibits neuronal cell death by targeting TRAF3 in cerebral ischemic stroke |
title_full_unstemmed | miR-455 inhibits neuronal cell death by targeting TRAF3 in cerebral ischemic stroke |
title_short | miR-455 inhibits neuronal cell death by targeting TRAF3 in cerebral ischemic stroke |
title_sort | mir-455 inhibits neuronal cell death by targeting traf3 in cerebral ischemic stroke |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5147416/ https://www.ncbi.nlm.nih.gov/pubmed/27980410 http://dx.doi.org/10.2147/NDT.S121183 |
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