Microspore Induced Doubled Haploids Production from Ethyl Methanesulfonate (EMS) Soaked Flower Buds Is an Efficient Strategy for Mutagenesis in Chinese Cabbage

Chinese cabbage buds were soaked with Ethyl methanesulfonate (EMS) to induce mutagenesis. The influence of different EMS concentrations and treatment durations on microspore development, embryo production rate and seedling rate were evaluated in five Chinese cabbage genotypes. Mutations in four colo...

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Autores principales: Lu, Yin, Dai, Shuangyan, Gu, Aixia, Liu, Mengyang, Wang, Yanhua, Luo, Shuangxia, Zhao, Yujing, Wang, Shan, Xuan, Shuxin, Chen, Xueping, Li, Xiaofeng, Bonnema, Guusje, Zhao, Jianjun, Shen, Shuxing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5147456/
https://www.ncbi.nlm.nih.gov/pubmed/28018368
http://dx.doi.org/10.3389/fpls.2016.01780
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author Lu, Yin
Dai, Shuangyan
Gu, Aixia
Liu, Mengyang
Wang, Yanhua
Luo, Shuangxia
Zhao, Yujing
Wang, Shan
Xuan, Shuxin
Chen, Xueping
Li, Xiaofeng
Bonnema, Guusje
Zhao, Jianjun
Shen, Shuxing
author_facet Lu, Yin
Dai, Shuangyan
Gu, Aixia
Liu, Mengyang
Wang, Yanhua
Luo, Shuangxia
Zhao, Yujing
Wang, Shan
Xuan, Shuxin
Chen, Xueping
Li, Xiaofeng
Bonnema, Guusje
Zhao, Jianjun
Shen, Shuxing
author_sort Lu, Yin
collection PubMed
description Chinese cabbage buds were soaked with Ethyl methanesulfonate (EMS) to induce mutagenesis. The influence of different EMS concentrations and treatment durations on microspore development, embryo production rate and seedling rate were evaluated in five Chinese cabbage genotypes. Mutations in four color-related genes were identified using high resolution melting (HRM) curves of their PCR products. The greatest embryo production and seedling rates were observed when buds were treated with 0.03 to 0.1% EMS for 5 to 10 min, while EMS concentrations greater than 0.1% were lethal to the microspores. In total, 142 mutants with distinct variations in leaf shape, leaf color, corolla size, flower color, bolting time and downy mildew resistance were identified from 475 microspore culture derived Doubled Haploids. Our results demonstrate that microspore derived Doubled Haploids from EMS soaked buds represents an efficient approach to rapidly generate homozygous Chinese cabbage mutants.
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spelling pubmed-51474562016-12-23 Microspore Induced Doubled Haploids Production from Ethyl Methanesulfonate (EMS) Soaked Flower Buds Is an Efficient Strategy for Mutagenesis in Chinese Cabbage Lu, Yin Dai, Shuangyan Gu, Aixia Liu, Mengyang Wang, Yanhua Luo, Shuangxia Zhao, Yujing Wang, Shan Xuan, Shuxin Chen, Xueping Li, Xiaofeng Bonnema, Guusje Zhao, Jianjun Shen, Shuxing Front Plant Sci Plant Science Chinese cabbage buds were soaked with Ethyl methanesulfonate (EMS) to induce mutagenesis. The influence of different EMS concentrations and treatment durations on microspore development, embryo production rate and seedling rate were evaluated in five Chinese cabbage genotypes. Mutations in four color-related genes were identified using high resolution melting (HRM) curves of their PCR products. The greatest embryo production and seedling rates were observed when buds were treated with 0.03 to 0.1% EMS for 5 to 10 min, while EMS concentrations greater than 0.1% were lethal to the microspores. In total, 142 mutants with distinct variations in leaf shape, leaf color, corolla size, flower color, bolting time and downy mildew resistance were identified from 475 microspore culture derived Doubled Haploids. Our results demonstrate that microspore derived Doubled Haploids from EMS soaked buds represents an efficient approach to rapidly generate homozygous Chinese cabbage mutants. Frontiers Media S.A. 2016-11-28 /pmc/articles/PMC5147456/ /pubmed/28018368 http://dx.doi.org/10.3389/fpls.2016.01780 Text en Copyright © 2016 Lu, Dai, Gu, Liu, Wang, Luo, Zhao, Wang, Xuan, Chen, Li, Bonnema, Zhao and Shen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Lu, Yin
Dai, Shuangyan
Gu, Aixia
Liu, Mengyang
Wang, Yanhua
Luo, Shuangxia
Zhao, Yujing
Wang, Shan
Xuan, Shuxin
Chen, Xueping
Li, Xiaofeng
Bonnema, Guusje
Zhao, Jianjun
Shen, Shuxing
Microspore Induced Doubled Haploids Production from Ethyl Methanesulfonate (EMS) Soaked Flower Buds Is an Efficient Strategy for Mutagenesis in Chinese Cabbage
title Microspore Induced Doubled Haploids Production from Ethyl Methanesulfonate (EMS) Soaked Flower Buds Is an Efficient Strategy for Mutagenesis in Chinese Cabbage
title_full Microspore Induced Doubled Haploids Production from Ethyl Methanesulfonate (EMS) Soaked Flower Buds Is an Efficient Strategy for Mutagenesis in Chinese Cabbage
title_fullStr Microspore Induced Doubled Haploids Production from Ethyl Methanesulfonate (EMS) Soaked Flower Buds Is an Efficient Strategy for Mutagenesis in Chinese Cabbage
title_full_unstemmed Microspore Induced Doubled Haploids Production from Ethyl Methanesulfonate (EMS) Soaked Flower Buds Is an Efficient Strategy for Mutagenesis in Chinese Cabbage
title_short Microspore Induced Doubled Haploids Production from Ethyl Methanesulfonate (EMS) Soaked Flower Buds Is an Efficient Strategy for Mutagenesis in Chinese Cabbage
title_sort microspore induced doubled haploids production from ethyl methanesulfonate (ems) soaked flower buds is an efficient strategy for mutagenesis in chinese cabbage
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5147456/
https://www.ncbi.nlm.nih.gov/pubmed/28018368
http://dx.doi.org/10.3389/fpls.2016.01780
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