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Recreating Stable Brachypodium hybridum Allotetraploids by Uniting the Divergent Genomes of B. distachyon and B. stacei

Brachypodium hybridum (2n = 30) is a natural allopolyploid with highly divergent sub-genomes derived from two extant diploid species, B. distachyon (2n = 10) and B. stacei (2n = 20) that differ in chromosome evolution and number. We created synthetic B. hybridum allotetraploids by hybridizing variou...

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Autores principales: Dinh Thi, Vinh Ha, Coriton, Olivier, Le Clainche, Isabelle, Arnaud, Dominique, Gordon, Sean P., Linc, Gabriella, Catalan, Pilar, Hasterok, Robert, Vogel, John P., Jahier, Joseph, Chalhoub, Boulos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5147888/
https://www.ncbi.nlm.nih.gov/pubmed/27936041
http://dx.doi.org/10.1371/journal.pone.0167171
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author Dinh Thi, Vinh Ha
Coriton, Olivier
Le Clainche, Isabelle
Arnaud, Dominique
Gordon, Sean P.
Linc, Gabriella
Catalan, Pilar
Hasterok, Robert
Vogel, John P.
Jahier, Joseph
Chalhoub, Boulos
author_facet Dinh Thi, Vinh Ha
Coriton, Olivier
Le Clainche, Isabelle
Arnaud, Dominique
Gordon, Sean P.
Linc, Gabriella
Catalan, Pilar
Hasterok, Robert
Vogel, John P.
Jahier, Joseph
Chalhoub, Boulos
author_sort Dinh Thi, Vinh Ha
collection PubMed
description Brachypodium hybridum (2n = 30) is a natural allopolyploid with highly divergent sub-genomes derived from two extant diploid species, B. distachyon (2n = 10) and B. stacei (2n = 20) that differ in chromosome evolution and number. We created synthetic B. hybridum allotetraploids by hybridizing various lines of B. distachyon and B. stacei. The initial amphihaploid F1 interspecific hybrids were obtained at low frequencies when B. distachyon was used as the maternal parent (0.15% or 0.245% depending on the line used) and were sterile. No hybrids were obtained from reciprocal crosses or when autotetraploids of the parental species were crossed. Colchicine treatment was used to double the genome of the F1 amphihaploid lines leading to allotetraploids. The genome-doubled F1 plants produced a few S1 (first selfed generation) seeds after self-pollination. S1 plants from one parental combination (Bd3-1×Bsta5) were fertile and gave rise to further generations whereas those of another parental combination (Bd21×ABR114) were sterile, illustrating the importance of the parental lineages crossed. The synthetic allotetraploids were stable and resembled the natural B. hybridum at the phenotypic, cytogenetic and genomic levels. The successful creation of synthetic B. hybridum offers the possibility to study changes in genome structure and regulation at the earliest stages of allopolyploid formation in comparison with the parental species and natural B. hybridum.
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spelling pubmed-51478882016-12-28 Recreating Stable Brachypodium hybridum Allotetraploids by Uniting the Divergent Genomes of B. distachyon and B. stacei Dinh Thi, Vinh Ha Coriton, Olivier Le Clainche, Isabelle Arnaud, Dominique Gordon, Sean P. Linc, Gabriella Catalan, Pilar Hasterok, Robert Vogel, John P. Jahier, Joseph Chalhoub, Boulos PLoS One Research Article Brachypodium hybridum (2n = 30) is a natural allopolyploid with highly divergent sub-genomes derived from two extant diploid species, B. distachyon (2n = 10) and B. stacei (2n = 20) that differ in chromosome evolution and number. We created synthetic B. hybridum allotetraploids by hybridizing various lines of B. distachyon and B. stacei. The initial amphihaploid F1 interspecific hybrids were obtained at low frequencies when B. distachyon was used as the maternal parent (0.15% or 0.245% depending on the line used) and were sterile. No hybrids were obtained from reciprocal crosses or when autotetraploids of the parental species were crossed. Colchicine treatment was used to double the genome of the F1 amphihaploid lines leading to allotetraploids. The genome-doubled F1 plants produced a few S1 (first selfed generation) seeds after self-pollination. S1 plants from one parental combination (Bd3-1×Bsta5) were fertile and gave rise to further generations whereas those of another parental combination (Bd21×ABR114) were sterile, illustrating the importance of the parental lineages crossed. The synthetic allotetraploids were stable and resembled the natural B. hybridum at the phenotypic, cytogenetic and genomic levels. The successful creation of synthetic B. hybridum offers the possibility to study changes in genome structure and regulation at the earliest stages of allopolyploid formation in comparison with the parental species and natural B. hybridum. Public Library of Science 2016-12-09 /pmc/articles/PMC5147888/ /pubmed/27936041 http://dx.doi.org/10.1371/journal.pone.0167171 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Dinh Thi, Vinh Ha
Coriton, Olivier
Le Clainche, Isabelle
Arnaud, Dominique
Gordon, Sean P.
Linc, Gabriella
Catalan, Pilar
Hasterok, Robert
Vogel, John P.
Jahier, Joseph
Chalhoub, Boulos
Recreating Stable Brachypodium hybridum Allotetraploids by Uniting the Divergent Genomes of B. distachyon and B. stacei
title Recreating Stable Brachypodium hybridum Allotetraploids by Uniting the Divergent Genomes of B. distachyon and B. stacei
title_full Recreating Stable Brachypodium hybridum Allotetraploids by Uniting the Divergent Genomes of B. distachyon and B. stacei
title_fullStr Recreating Stable Brachypodium hybridum Allotetraploids by Uniting the Divergent Genomes of B. distachyon and B. stacei
title_full_unstemmed Recreating Stable Brachypodium hybridum Allotetraploids by Uniting the Divergent Genomes of B. distachyon and B. stacei
title_short Recreating Stable Brachypodium hybridum Allotetraploids by Uniting the Divergent Genomes of B. distachyon and B. stacei
title_sort recreating stable brachypodium hybridum allotetraploids by uniting the divergent genomes of b. distachyon and b. stacei
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5147888/
https://www.ncbi.nlm.nih.gov/pubmed/27936041
http://dx.doi.org/10.1371/journal.pone.0167171
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