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Single synapse evaluation of the postsynaptic NMDA receptors targeted by evoked and spontaneous neurotransmission

Recent studies indicate that within individual synapses spontaneous and evoked release processes are segregated and regulated independently. In the hippocampus, earlier electrophysiological recordings suggested that spontaneous and evoked glutamate release can activate separate groups of postsynapti...

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Detalles Bibliográficos
Autores principales: Reese, Austin L, Kavalali, Ege T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5148599/
https://www.ncbi.nlm.nih.gov/pubmed/27882871
http://dx.doi.org/10.7554/eLife.21170
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author Reese, Austin L
Kavalali, Ege T
author_facet Reese, Austin L
Kavalali, Ege T
author_sort Reese, Austin L
collection PubMed
description Recent studies indicate that within individual synapses spontaneous and evoked release processes are segregated and regulated independently. In the hippocampus, earlier electrophysiological recordings suggested that spontaneous and evoked glutamate release can activate separate groups of postsynaptic NMDA receptors with limited overlap. However, it is still unclear how this separation of NMDA receptors is distributed across individual synapses. In a previous paper (Reese and Kavalali, 2015) we showed that NMDA receptor mediated spontaneous transmission signals to the postsynaptic protein translation machinery through Ca(2+)-induced Ca(2+) release. Here, we show that in rat hippocampal neurons although spontaneous and evoked glutamate release driven NMDA receptor mediated Ca2+ transients often occur at the same synapse, these two signals do not show significant correlation or cross talk. DOI: http://dx.doi.org/10.7554/eLife.21170.001
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spelling pubmed-51485992016-12-12 Single synapse evaluation of the postsynaptic NMDA receptors targeted by evoked and spontaneous neurotransmission Reese, Austin L Kavalali, Ege T eLife Neuroscience Recent studies indicate that within individual synapses spontaneous and evoked release processes are segregated and regulated independently. In the hippocampus, earlier electrophysiological recordings suggested that spontaneous and evoked glutamate release can activate separate groups of postsynaptic NMDA receptors with limited overlap. However, it is still unclear how this separation of NMDA receptors is distributed across individual synapses. In a previous paper (Reese and Kavalali, 2015) we showed that NMDA receptor mediated spontaneous transmission signals to the postsynaptic protein translation machinery through Ca(2+)-induced Ca(2+) release. Here, we show that in rat hippocampal neurons although spontaneous and evoked glutamate release driven NMDA receptor mediated Ca2+ transients often occur at the same synapse, these two signals do not show significant correlation or cross talk. DOI: http://dx.doi.org/10.7554/eLife.21170.001 eLife Sciences Publications, Ltd 2016-11-24 /pmc/articles/PMC5148599/ /pubmed/27882871 http://dx.doi.org/10.7554/eLife.21170 Text en © 2016, Reese et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Reese, Austin L
Kavalali, Ege T
Single synapse evaluation of the postsynaptic NMDA receptors targeted by evoked and spontaneous neurotransmission
title Single synapse evaluation of the postsynaptic NMDA receptors targeted by evoked and spontaneous neurotransmission
title_full Single synapse evaluation of the postsynaptic NMDA receptors targeted by evoked and spontaneous neurotransmission
title_fullStr Single synapse evaluation of the postsynaptic NMDA receptors targeted by evoked and spontaneous neurotransmission
title_full_unstemmed Single synapse evaluation of the postsynaptic NMDA receptors targeted by evoked and spontaneous neurotransmission
title_short Single synapse evaluation of the postsynaptic NMDA receptors targeted by evoked and spontaneous neurotransmission
title_sort single synapse evaluation of the postsynaptic nmda receptors targeted by evoked and spontaneous neurotransmission
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5148599/
https://www.ncbi.nlm.nih.gov/pubmed/27882871
http://dx.doi.org/10.7554/eLife.21170
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