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Hormone-sensitive lipase deficiency alters gene expression and cholesterol content of mouse testis

Hormone-sensitive lipase-knockout (HSL−/−) mice exhibit azoospermia for unclear reasons. To explore the basis of sterility, we performed the following three experiments. First, HSL protein distribution in the testis was determined. Next, transcriptome analyses were performed on the testes of three e...

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Autores principales: Wang, Feng, Chen, Zheng, Ren, Xiaofang, Tian, Ye, Wang, Fucheng, Liu, Chao, Jin, Pengcheng, Li, Zongyue, Zhang, Feixiong, Zhu, Baochang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bioscientifica Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5148802/
https://www.ncbi.nlm.nih.gov/pubmed/27920259
http://dx.doi.org/10.1530/REP-16-0484
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author Wang, Feng
Chen, Zheng
Ren, Xiaofang
Tian, Ye
Wang, Fucheng
Liu, Chao
Jin, Pengcheng
Li, Zongyue
Zhang, Feixiong
Zhu, Baochang
author_facet Wang, Feng
Chen, Zheng
Ren, Xiaofang
Tian, Ye
Wang, Fucheng
Liu, Chao
Jin, Pengcheng
Li, Zongyue
Zhang, Feixiong
Zhu, Baochang
author_sort Wang, Feng
collection PubMed
description Hormone-sensitive lipase-knockout (HSL−/−) mice exhibit azoospermia for unclear reasons. To explore the basis of sterility, we performed the following three experiments. First, HSL protein distribution in the testis was determined. Next, transcriptome analyses were performed on the testes of three experimental groups. Finally, the fatty acid and cholesterol levels in the testes with three different genotypes studied were determined. We found that the HSL protein was present from spermatocyte cells to mature sperm acrosomes in wild-type (HSL+/+) testes. Spermiogenesis ceased at the elongation phase of HSL−/− testes. Transcriptome analysis indicated that genes involved in lipid metabolism, cell membrane, reproduction and inflammation-related processes were disordered in HSL−/− testes. The cholesterol content was significantly higher in HSL−/− than that in HSL+/+ testis. Therefore, gene expression and cholesterol ester content differed in HSL−/− testes compared to other testes, which may explain the sterility of male HSL−/− mice.
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spelling pubmed-51488022016-12-19 Hormone-sensitive lipase deficiency alters gene expression and cholesterol content of mouse testis Wang, Feng Chen, Zheng Ren, Xiaofang Tian, Ye Wang, Fucheng Liu, Chao Jin, Pengcheng Li, Zongyue Zhang, Feixiong Zhu, Baochang Reproduction Research Hormone-sensitive lipase-knockout (HSL−/−) mice exhibit azoospermia for unclear reasons. To explore the basis of sterility, we performed the following three experiments. First, HSL protein distribution in the testis was determined. Next, transcriptome analyses were performed on the testes of three experimental groups. Finally, the fatty acid and cholesterol levels in the testes with three different genotypes studied were determined. We found that the HSL protein was present from spermatocyte cells to mature sperm acrosomes in wild-type (HSL+/+) testes. Spermiogenesis ceased at the elongation phase of HSL−/− testes. Transcriptome analysis indicated that genes involved in lipid metabolism, cell membrane, reproduction and inflammation-related processes were disordered in HSL−/− testes. The cholesterol content was significantly higher in HSL−/− than that in HSL+/+ testis. Therefore, gene expression and cholesterol ester content differed in HSL−/− testes compared to other testes, which may explain the sterility of male HSL−/− mice. Bioscientifica Ltd 2016-12-05 /pmc/articles/PMC5148802/ /pubmed/27920259 http://dx.doi.org/10.1530/REP-16-0484 Text en © 2017 The Authors http://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Research
Wang, Feng
Chen, Zheng
Ren, Xiaofang
Tian, Ye
Wang, Fucheng
Liu, Chao
Jin, Pengcheng
Li, Zongyue
Zhang, Feixiong
Zhu, Baochang
Hormone-sensitive lipase deficiency alters gene expression and cholesterol content of mouse testis
title Hormone-sensitive lipase deficiency alters gene expression and cholesterol content of mouse testis
title_full Hormone-sensitive lipase deficiency alters gene expression and cholesterol content of mouse testis
title_fullStr Hormone-sensitive lipase deficiency alters gene expression and cholesterol content of mouse testis
title_full_unstemmed Hormone-sensitive lipase deficiency alters gene expression and cholesterol content of mouse testis
title_short Hormone-sensitive lipase deficiency alters gene expression and cholesterol content of mouse testis
title_sort hormone-sensitive lipase deficiency alters gene expression and cholesterol content of mouse testis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5148802/
https://www.ncbi.nlm.nih.gov/pubmed/27920259
http://dx.doi.org/10.1530/REP-16-0484
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