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Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration
BACKGROUND: The rapid and reliable detection of infectious agents is one of the most challenging tasks in scenarios lacking well-equipped laboratory infrastructure, like diagnostics in rural areas of developing countries. Commercially available point-of-care diagnostic tests for emerging and rare di...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5148848/ https://www.ncbi.nlm.nih.gov/pubmed/27938377 http://dx.doi.org/10.1186/s12985-016-0665-5 |
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author | Stern, Daniel Olson, Victoria A. Smith, Scott K. Pietraszczyk, Marko Miller, Lilija Miethe, Peter Dorner, Brigitte G. Nitsche, Andreas |
author_facet | Stern, Daniel Olson, Victoria A. Smith, Scott K. Pietraszczyk, Marko Miller, Lilija Miethe, Peter Dorner, Brigitte G. Nitsche, Andreas |
author_sort | Stern, Daniel |
collection | PubMed |
description | BACKGROUND: The rapid and reliable detection of infectious agents is one of the most challenging tasks in scenarios lacking well-equipped laboratory infrastructure, like diagnostics in rural areas of developing countries. Commercially available point-of-care diagnostic tests for emerging and rare diseases are particularly scarce. RESULTS: In this work we present a point-of-care test for the detection of Orthopoxviruses (OPV). The OPV ABICAP assay detects down to 1 × 10(4) plaque forming units/mL of OPV particles within 45 min. It can be applied to clinical material like skin crusts and detects all zoonotic OPV infecting humans, including Vaccinia, Cowpox, Monkeypox, and most importantly Variola virus. CONCLUSIONS: Given the high sensitivity and the ease of handling, the novel assay could be highly useful for on-site diagnostics of suspected Monkeypox virus infections in areas lacking proper laboratory infrastructure as well as rapid on-site testing of suspected bioterrorism samples. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12985-016-0665-5) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5148848 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-51488482016-12-15 Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration Stern, Daniel Olson, Victoria A. Smith, Scott K. Pietraszczyk, Marko Miller, Lilija Miethe, Peter Dorner, Brigitte G. Nitsche, Andreas Virol J Methodology BACKGROUND: The rapid and reliable detection of infectious agents is one of the most challenging tasks in scenarios lacking well-equipped laboratory infrastructure, like diagnostics in rural areas of developing countries. Commercially available point-of-care diagnostic tests for emerging and rare diseases are particularly scarce. RESULTS: In this work we present a point-of-care test for the detection of Orthopoxviruses (OPV). The OPV ABICAP assay detects down to 1 × 10(4) plaque forming units/mL of OPV particles within 45 min. It can be applied to clinical material like skin crusts and detects all zoonotic OPV infecting humans, including Vaccinia, Cowpox, Monkeypox, and most importantly Variola virus. CONCLUSIONS: Given the high sensitivity and the ease of handling, the novel assay could be highly useful for on-site diagnostics of suspected Monkeypox virus infections in areas lacking proper laboratory infrastructure as well as rapid on-site testing of suspected bioterrorism samples. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12985-016-0665-5) contains supplementary material, which is available to authorized users. BioMed Central 2016-12-09 /pmc/articles/PMC5148848/ /pubmed/27938377 http://dx.doi.org/10.1186/s12985-016-0665-5 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Methodology Stern, Daniel Olson, Victoria A. Smith, Scott K. Pietraszczyk, Marko Miller, Lilija Miethe, Peter Dorner, Brigitte G. Nitsche, Andreas Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration |
title | Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration |
title_full | Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration |
title_fullStr | Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration |
title_full_unstemmed | Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration |
title_short | Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration |
title_sort | rapid and sensitive point-of-care detection of orthopoxviruses by abicap immunofiltration |
topic | Methodology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5148848/ https://www.ncbi.nlm.nih.gov/pubmed/27938377 http://dx.doi.org/10.1186/s12985-016-0665-5 |
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