Cargando…

Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration

BACKGROUND: The rapid and reliable detection of infectious agents is one of the most challenging tasks in scenarios lacking well-equipped laboratory infrastructure, like diagnostics in rural areas of developing countries. Commercially available point-of-care diagnostic tests for emerging and rare di...

Descripción completa

Detalles Bibliográficos
Autores principales: Stern, Daniel, Olson, Victoria A., Smith, Scott K., Pietraszczyk, Marko, Miller, Lilija, Miethe, Peter, Dorner, Brigitte G., Nitsche, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5148848/
https://www.ncbi.nlm.nih.gov/pubmed/27938377
http://dx.doi.org/10.1186/s12985-016-0665-5
_version_ 1782473893972279296
author Stern, Daniel
Olson, Victoria A.
Smith, Scott K.
Pietraszczyk, Marko
Miller, Lilija
Miethe, Peter
Dorner, Brigitte G.
Nitsche, Andreas
author_facet Stern, Daniel
Olson, Victoria A.
Smith, Scott K.
Pietraszczyk, Marko
Miller, Lilija
Miethe, Peter
Dorner, Brigitte G.
Nitsche, Andreas
author_sort Stern, Daniel
collection PubMed
description BACKGROUND: The rapid and reliable detection of infectious agents is one of the most challenging tasks in scenarios lacking well-equipped laboratory infrastructure, like diagnostics in rural areas of developing countries. Commercially available point-of-care diagnostic tests for emerging and rare diseases are particularly scarce. RESULTS: In this work we present a point-of-care test for the detection of Orthopoxviruses (OPV). The OPV ABICAP assay detects down to 1 × 10(4) plaque forming units/mL of OPV particles within 45 min. It can be applied to clinical material like skin crusts and detects all zoonotic OPV infecting humans, including Vaccinia, Cowpox, Monkeypox, and most importantly Variola virus. CONCLUSIONS: Given the high sensitivity and the ease of handling, the novel assay could be highly useful for on-site diagnostics of suspected Monkeypox virus infections in areas lacking proper laboratory infrastructure as well as rapid on-site testing of suspected bioterrorism samples. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12985-016-0665-5) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5148848
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-51488482016-12-15 Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration Stern, Daniel Olson, Victoria A. Smith, Scott K. Pietraszczyk, Marko Miller, Lilija Miethe, Peter Dorner, Brigitte G. Nitsche, Andreas Virol J Methodology BACKGROUND: The rapid and reliable detection of infectious agents is one of the most challenging tasks in scenarios lacking well-equipped laboratory infrastructure, like diagnostics in rural areas of developing countries. Commercially available point-of-care diagnostic tests for emerging and rare diseases are particularly scarce. RESULTS: In this work we present a point-of-care test for the detection of Orthopoxviruses (OPV). The OPV ABICAP assay detects down to 1 × 10(4) plaque forming units/mL of OPV particles within 45 min. It can be applied to clinical material like skin crusts and detects all zoonotic OPV infecting humans, including Vaccinia, Cowpox, Monkeypox, and most importantly Variola virus. CONCLUSIONS: Given the high sensitivity and the ease of handling, the novel assay could be highly useful for on-site diagnostics of suspected Monkeypox virus infections in areas lacking proper laboratory infrastructure as well as rapid on-site testing of suspected bioterrorism samples. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12985-016-0665-5) contains supplementary material, which is available to authorized users. BioMed Central 2016-12-09 /pmc/articles/PMC5148848/ /pubmed/27938377 http://dx.doi.org/10.1186/s12985-016-0665-5 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Methodology
Stern, Daniel
Olson, Victoria A.
Smith, Scott K.
Pietraszczyk, Marko
Miller, Lilija
Miethe, Peter
Dorner, Brigitte G.
Nitsche, Andreas
Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration
title Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration
title_full Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration
title_fullStr Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration
title_full_unstemmed Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration
title_short Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration
title_sort rapid and sensitive point-of-care detection of orthopoxviruses by abicap immunofiltration
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5148848/
https://www.ncbi.nlm.nih.gov/pubmed/27938377
http://dx.doi.org/10.1186/s12985-016-0665-5
work_keys_str_mv AT sterndaniel rapidandsensitivepointofcaredetectionoforthopoxvirusesbyabicapimmunofiltration
AT olsonvictoriaa rapidandsensitivepointofcaredetectionoforthopoxvirusesbyabicapimmunofiltration
AT smithscottk rapidandsensitivepointofcaredetectionoforthopoxvirusesbyabicapimmunofiltration
AT pietraszczykmarko rapidandsensitivepointofcaredetectionoforthopoxvirusesbyabicapimmunofiltration
AT millerlilija rapidandsensitivepointofcaredetectionoforthopoxvirusesbyabicapimmunofiltration
AT miethepeter rapidandsensitivepointofcaredetectionoforthopoxvirusesbyabicapimmunofiltration
AT dornerbrigitteg rapidandsensitivepointofcaredetectionoforthopoxvirusesbyabicapimmunofiltration
AT nitscheandreas rapidandsensitivepointofcaredetectionoforthopoxvirusesbyabicapimmunofiltration