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PET imaging-guided chemogenetic silencing reveals a critical role of primate rostromedial caudate in reward evaluation

The rostromedial caudate (rmCD) of primates is thought to contribute to reward value processing, but a causal relationship has not been established. Here we use an inhibitory DREADD (Designer Receptor Exclusively Activated by Designer Drug) to repeatedly and non-invasively inactivate rmCD of macaque...

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Detalles Bibliográficos
Autores principales: Nagai, Yuji, Kikuchi, Erika, Lerchner, Walter, Inoue, Ken-ichi, Ji, Bin, Eldridge, Mark A. G., Kaneko, Hiroyuki, Kimura, Yasuyuki, Oh-Nishi, Arata, Hori, Yukiko, Kato, Yoko, Hirabayashi, Toshiyuki, Fujimoto, Atsushi, Kumata, Katsushi, Zhang, Ming-Rong, Aoki, Ichio, Suhara, Tetsuya, Higuchi, Makoto, Takada, Masahiko, Richmond, Barry J., Minamimoto, Takafumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5150653/
https://www.ncbi.nlm.nih.gov/pubmed/27922009
http://dx.doi.org/10.1038/ncomms13605
Descripción
Sumario:The rostromedial caudate (rmCD) of primates is thought to contribute to reward value processing, but a causal relationship has not been established. Here we use an inhibitory DREADD (Designer Receptor Exclusively Activated by Designer Drug) to repeatedly and non-invasively inactivate rmCD of macaque monkeys. We inject an adeno-associated viral vector expressing the inhibitory DREADD, hM4Di, into the rmCD bilaterally. To visualize DREADD expression in vivo, we develop a non-invasive imaging method using positron emission tomography (PET). PET imaging provides information critical for successful chemogenetic silencing during experiments, in this case the location and level of hM4Di expression, and the relationship between agonist dose and hM4Di receptor occupancy. Here we demonstrate that inactivating bilateral rmCD through activation of hM4Di produces a significant and reproducible loss of sensitivity to reward value in monkeys. Thus, the rmCD is involved in making normal judgments about the value of reward.