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Engineering of chitosan and collagen macromolecules using sebacic acid for clinical applications

Transformation of natural polymers to three-dimensional (3D) scaffolds for biomedical applications faces a number of challenges, viz., solubility, stability (mechanical and thermal), strength, biocompatibility, and biodegradability. Hence, intensive research on suitable agents to provide the requisi...

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Autores principales: Sailakshmi, G, Mitra, Tapas, Gnanamani, A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5151108/
https://www.ncbi.nlm.nih.gov/pubmed/29470652
http://dx.doi.org/10.1186/2194-0517-2-11
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author Sailakshmi, G
Mitra, Tapas
Gnanamani, A
author_facet Sailakshmi, G
Mitra, Tapas
Gnanamani, A
author_sort Sailakshmi, G
collection PubMed
description Transformation of natural polymers to three-dimensional (3D) scaffolds for biomedical applications faces a number of challenges, viz., solubility, stability (mechanical and thermal), strength, biocompatibility, and biodegradability. Hence, intensive research on suitable agents to provide the requisite properties has been initiated at the global level. In the present study, an attempt was made to engineer chitosan and collagen macromolecules using sebacic acid, and further evaluation of the mechanical stability and biocompatible property of the engineered scaffold material was done. A 3D scaffold material was prepared using chitosan at 1.0% (w/v) and sebacic acid at 0.2% (w/v); similarly, collagen at 0.5% (w/v) and sebacic acid at 0.2% (w/v) were prepared individually by freeze-drying technique. Analysis revealed that the engineered scaffolds displayed an appreciable mechanical strength and, in addition, were found to be biocompatible to NIH 3T3 fibroblast cells. Studies on the chemistry behind the interaction and the characteristics of the cross-linked scaffold materials suggested that non-covalent interactions play a major role in deciding the property of the said polymer materials. The prepared scaffold was suitable for tissue engineering application as a wound dressing material. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/2194-0517-2-11) contains supplementary material, which is available to authorized users.
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spelling pubmed-51511082016-12-27 Engineering of chitosan and collagen macromolecules using sebacic acid for clinical applications Sailakshmi, G Mitra, Tapas Gnanamani, A Prog Biomater Original Research Transformation of natural polymers to three-dimensional (3D) scaffolds for biomedical applications faces a number of challenges, viz., solubility, stability (mechanical and thermal), strength, biocompatibility, and biodegradability. Hence, intensive research on suitable agents to provide the requisite properties has been initiated at the global level. In the present study, an attempt was made to engineer chitosan and collagen macromolecules using sebacic acid, and further evaluation of the mechanical stability and biocompatible property of the engineered scaffold material was done. A 3D scaffold material was prepared using chitosan at 1.0% (w/v) and sebacic acid at 0.2% (w/v); similarly, collagen at 0.5% (w/v) and sebacic acid at 0.2% (w/v) were prepared individually by freeze-drying technique. Analysis revealed that the engineered scaffolds displayed an appreciable mechanical strength and, in addition, were found to be biocompatible to NIH 3T3 fibroblast cells. Studies on the chemistry behind the interaction and the characteristics of the cross-linked scaffold materials suggested that non-covalent interactions play a major role in deciding the property of the said polymer materials. The prepared scaffold was suitable for tissue engineering application as a wound dressing material. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/2194-0517-2-11) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2013-04-23 /pmc/articles/PMC5151108/ /pubmed/29470652 http://dx.doi.org/10.1186/2194-0517-2-11 Text en © Sailakshmi et al.; licensee Springer. 2013 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Sailakshmi, G
Mitra, Tapas
Gnanamani, A
Engineering of chitosan and collagen macromolecules using sebacic acid for clinical applications
title Engineering of chitosan and collagen macromolecules using sebacic acid for clinical applications
title_full Engineering of chitosan and collagen macromolecules using sebacic acid for clinical applications
title_fullStr Engineering of chitosan and collagen macromolecules using sebacic acid for clinical applications
title_full_unstemmed Engineering of chitosan and collagen macromolecules using sebacic acid for clinical applications
title_short Engineering of chitosan and collagen macromolecules using sebacic acid for clinical applications
title_sort engineering of chitosan and collagen macromolecules using sebacic acid for clinical applications
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5151108/
https://www.ncbi.nlm.nih.gov/pubmed/29470652
http://dx.doi.org/10.1186/2194-0517-2-11
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