Cargando…

Leukotriene B4 levels in sputum from asthma patients

Poor asthma control is associated with increased airway neutrophils. Leukotriene B4 (LTB4) is a potent neutrophil chemoattractant. We examined the levels of LTB4 levels in the sputum of asthma patients and the relationship with disease severity. 47 asthma patients (categorised according to Global In...

Descripción completa

Detalles Bibliográficos
Autores principales: Higham, Andrew, Cadden, Paul, Southworth, Thomas, Rossall, Matthew, Kolsum, Umme, Lea, Simon, Knowles, Richard, Singh, Dave
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Respiratory Society 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5152838/
https://www.ncbi.nlm.nih.gov/pubmed/28053970
http://dx.doi.org/10.1183/23120541.00088-2015
_version_ 1782474629394202624
author Higham, Andrew
Cadden, Paul
Southworth, Thomas
Rossall, Matthew
Kolsum, Umme
Lea, Simon
Knowles, Richard
Singh, Dave
author_facet Higham, Andrew
Cadden, Paul
Southworth, Thomas
Rossall, Matthew
Kolsum, Umme
Lea, Simon
Knowles, Richard
Singh, Dave
author_sort Higham, Andrew
collection PubMed
description Poor asthma control is associated with increased airway neutrophils. Leukotriene B4 (LTB4) is a potent neutrophil chemoattractant. We examined the levels of LTB4 levels in the sputum of asthma patients and the relationship with disease severity. 47 asthma patients (categorised according to Global Initiative for Asthma treatment stage) and 12 healthy controls provided sputum samples that were processed first with PBS to obtain supernatants and secondly with dithiothreitol (DTT) to obtain supernatants. LTB4 levels were determined by ELISA. LTB4 levels were significantly higher in step 1 (steroid naïve) and step 3 (inhaled corticosteroid (ICS) plus long acting β-agonist) patients than step 2 patients (ICS alone) (p=0.02 and p=0.01, respectively). There was very good correlation when comparing PBS processed to DTT processed supernatants. High LTB4 levels were found in the sputum of asthmatics at step 3 despite ICS use.
format Online
Article
Text
id pubmed-5152838
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher European Respiratory Society
record_format MEDLINE/PubMed
spelling pubmed-51528382017-01-04 Leukotriene B4 levels in sputum from asthma patients Higham, Andrew Cadden, Paul Southworth, Thomas Rossall, Matthew Kolsum, Umme Lea, Simon Knowles, Richard Singh, Dave ERJ Open Res Original Articles Poor asthma control is associated with increased airway neutrophils. Leukotriene B4 (LTB4) is a potent neutrophil chemoattractant. We examined the levels of LTB4 levels in the sputum of asthma patients and the relationship with disease severity. 47 asthma patients (categorised according to Global Initiative for Asthma treatment stage) and 12 healthy controls provided sputum samples that were processed first with PBS to obtain supernatants and secondly with dithiothreitol (DTT) to obtain supernatants. LTB4 levels were determined by ELISA. LTB4 levels were significantly higher in step 1 (steroid naïve) and step 3 (inhaled corticosteroid (ICS) plus long acting β-agonist) patients than step 2 patients (ICS alone) (p=0.02 and p=0.01, respectively). There was very good correlation when comparing PBS processed to DTT processed supernatants. High LTB4 levels were found in the sputum of asthmatics at step 3 despite ICS use. European Respiratory Society 2016-10-26 /pmc/articles/PMC5152838/ /pubmed/28053970 http://dx.doi.org/10.1183/23120541.00088-2015 Text en Copyright ©ERS 2016 http://creativecommons.org/licenses/by-nc/4.0/ This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0.
spellingShingle Original Articles
Higham, Andrew
Cadden, Paul
Southworth, Thomas
Rossall, Matthew
Kolsum, Umme
Lea, Simon
Knowles, Richard
Singh, Dave
Leukotriene B4 levels in sputum from asthma patients
title Leukotriene B4 levels in sputum from asthma patients
title_full Leukotriene B4 levels in sputum from asthma patients
title_fullStr Leukotriene B4 levels in sputum from asthma patients
title_full_unstemmed Leukotriene B4 levels in sputum from asthma patients
title_short Leukotriene B4 levels in sputum from asthma patients
title_sort leukotriene b4 levels in sputum from asthma patients
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5152838/
https://www.ncbi.nlm.nih.gov/pubmed/28053970
http://dx.doi.org/10.1183/23120541.00088-2015
work_keys_str_mv AT highamandrew leukotrieneb4levelsinsputumfromasthmapatients
AT caddenpaul leukotrieneb4levelsinsputumfromasthmapatients
AT southworththomas leukotrieneb4levelsinsputumfromasthmapatients
AT rossallmatthew leukotrieneb4levelsinsputumfromasthmapatients
AT kolsumumme leukotrieneb4levelsinsputumfromasthmapatients
AT leasimon leukotrieneb4levelsinsputumfromasthmapatients
AT knowlesrichard leukotrieneb4levelsinsputumfromasthmapatients
AT singhdave leukotrieneb4levelsinsputumfromasthmapatients