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Diagnostic test accuracy of loop-mediated isothermal amplification assay for Mycobacterium tuberculosis: systematic review and meta-analysis

Diagnostic test accuracy of the loop-mediated isothermal amplification (LAMP) assay for culture proven tuberculosis is unclear. We searched electronic databases for both cohort and case-control studies that provided data to calculate sensitivity and specificity. The index test was any LAMP assay inc...

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Autores principales: Nagai, Kenjiro, Horita, Nobuyuki, Yamamoto, Masaki, Tsukahara, Toshinori, Nagakura, Hideyuki, Tashiro, Ken, Shibata, Yuji, Watanabe, Hiroki, Nakashima, Kentaro, Ushio, Ryota, Ikeda, Misako, Narita, Atsuya, Kanai, Akinori, Sato, Takashi, Kaneko, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5153623/
https://www.ncbi.nlm.nih.gov/pubmed/27958360
http://dx.doi.org/10.1038/srep39090
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author Nagai, Kenjiro
Horita, Nobuyuki
Yamamoto, Masaki
Tsukahara, Toshinori
Nagakura, Hideyuki
Tashiro, Ken
Shibata, Yuji
Watanabe, Hiroki
Nakashima, Kentaro
Ushio, Ryota
Ikeda, Misako
Narita, Atsuya
Kanai, Akinori
Sato, Takashi
Kaneko, Takeshi
author_facet Nagai, Kenjiro
Horita, Nobuyuki
Yamamoto, Masaki
Tsukahara, Toshinori
Nagakura, Hideyuki
Tashiro, Ken
Shibata, Yuji
Watanabe, Hiroki
Nakashima, Kentaro
Ushio, Ryota
Ikeda, Misako
Narita, Atsuya
Kanai, Akinori
Sato, Takashi
Kaneko, Takeshi
author_sort Nagai, Kenjiro
collection PubMed
description Diagnostic test accuracy of the loop-mediated isothermal amplification (LAMP) assay for culture proven tuberculosis is unclear. We searched electronic databases for both cohort and case-control studies that provided data to calculate sensitivity and specificity. The index test was any LAMP assay including both commercialized kits and in-house assays. Culture-proven M. tuberculosis was considered a positive reference test. We included 26 studies on 9330 sputum samples and one study on 315 extra-pulmonary specimens. For sputum samples, 26 studies yielded the summary estimates of sensitivity of 89.6% (95% CI 85.6–92.6%), specificity of 94.0% (95% CI 91.0–96.1%), and a diagnostic odds ratio of 145 (95% CI 93–226). Nine studies focusing on Loopamp MTBC yielded the summary estimates of sensitivity of 80.9% (95% CI 76.0–85.1%) and specificity of 96.5% (95% CI 94.7–97.7%). Loopamp MTBC had higher sensitivity and lower specificity for smear-positive sputa compared to smear-negative sputa. In-house assays showed higher sensitivity and lower specificity compared to Loopamp MTBC. LAMP promises to be a useful test for the diagnosis of TB, however there is still need to improve the assay to make it simpler, cheaper and more efficient to make it competitive against other PCR methods already available.
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spelling pubmed-51536232016-12-19 Diagnostic test accuracy of loop-mediated isothermal amplification assay for Mycobacterium tuberculosis: systematic review and meta-analysis Nagai, Kenjiro Horita, Nobuyuki Yamamoto, Masaki Tsukahara, Toshinori Nagakura, Hideyuki Tashiro, Ken Shibata, Yuji Watanabe, Hiroki Nakashima, Kentaro Ushio, Ryota Ikeda, Misako Narita, Atsuya Kanai, Akinori Sato, Takashi Kaneko, Takeshi Sci Rep Article Diagnostic test accuracy of the loop-mediated isothermal amplification (LAMP) assay for culture proven tuberculosis is unclear. We searched electronic databases for both cohort and case-control studies that provided data to calculate sensitivity and specificity. The index test was any LAMP assay including both commercialized kits and in-house assays. Culture-proven M. tuberculosis was considered a positive reference test. We included 26 studies on 9330 sputum samples and one study on 315 extra-pulmonary specimens. For sputum samples, 26 studies yielded the summary estimates of sensitivity of 89.6% (95% CI 85.6–92.6%), specificity of 94.0% (95% CI 91.0–96.1%), and a diagnostic odds ratio of 145 (95% CI 93–226). Nine studies focusing on Loopamp MTBC yielded the summary estimates of sensitivity of 80.9% (95% CI 76.0–85.1%) and specificity of 96.5% (95% CI 94.7–97.7%). Loopamp MTBC had higher sensitivity and lower specificity for smear-positive sputa compared to smear-negative sputa. In-house assays showed higher sensitivity and lower specificity compared to Loopamp MTBC. LAMP promises to be a useful test for the diagnosis of TB, however there is still need to improve the assay to make it simpler, cheaper and more efficient to make it competitive against other PCR methods already available. Nature Publishing Group 2016-12-13 /pmc/articles/PMC5153623/ /pubmed/27958360 http://dx.doi.org/10.1038/srep39090 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Nagai, Kenjiro
Horita, Nobuyuki
Yamamoto, Masaki
Tsukahara, Toshinori
Nagakura, Hideyuki
Tashiro, Ken
Shibata, Yuji
Watanabe, Hiroki
Nakashima, Kentaro
Ushio, Ryota
Ikeda, Misako
Narita, Atsuya
Kanai, Akinori
Sato, Takashi
Kaneko, Takeshi
Diagnostic test accuracy of loop-mediated isothermal amplification assay for Mycobacterium tuberculosis: systematic review and meta-analysis
title Diagnostic test accuracy of loop-mediated isothermal amplification assay for Mycobacterium tuberculosis: systematic review and meta-analysis
title_full Diagnostic test accuracy of loop-mediated isothermal amplification assay for Mycobacterium tuberculosis: systematic review and meta-analysis
title_fullStr Diagnostic test accuracy of loop-mediated isothermal amplification assay for Mycobacterium tuberculosis: systematic review and meta-analysis
title_full_unstemmed Diagnostic test accuracy of loop-mediated isothermal amplification assay for Mycobacterium tuberculosis: systematic review and meta-analysis
title_short Diagnostic test accuracy of loop-mediated isothermal amplification assay for Mycobacterium tuberculosis: systematic review and meta-analysis
title_sort diagnostic test accuracy of loop-mediated isothermal amplification assay for mycobacterium tuberculosis: systematic review and meta-analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5153623/
https://www.ncbi.nlm.nih.gov/pubmed/27958360
http://dx.doi.org/10.1038/srep39090
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