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The effect of Chinese herbs and its effective components on coronary heart disease through PPARs-PGC1α pathway

BACKGROUND: DanQi pill (DQP) is prescribed widely in China and has definite cardioprotective effect on coronary heart disease. Our previous studies proved that DQP could effectively regulate plasma levels of high density lipoprotein (HDL) and low density lipoprotein (LDL). However, the regulatory me...

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Autores principales: Wang, Qiyan, Li, Chun, Zhang, Qian, Wang, Yuanyuan, Shi, Tianjiao, Lu, Linghui, Zhang, Yi, Wang, Yong, Wang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5153825/
https://www.ncbi.nlm.nih.gov/pubmed/27955667
http://dx.doi.org/10.1186/s12906-016-1496-z
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author Wang, Qiyan
Li, Chun
Zhang, Qian
Wang, Yuanyuan
Shi, Tianjiao
Lu, Linghui
Zhang, Yi
Wang, Yong
Wang, Wei
author_facet Wang, Qiyan
Li, Chun
Zhang, Qian
Wang, Yuanyuan
Shi, Tianjiao
Lu, Linghui
Zhang, Yi
Wang, Yong
Wang, Wei
author_sort Wang, Qiyan
collection PubMed
description BACKGROUND: DanQi pill (DQP) is prescribed widely in China and has definite cardioprotective effect on coronary heart disease. Our previous studies proved that DQP could effectively regulate plasma levels of high density lipoprotein (HDL) and low density lipoprotein (LDL). However, the regulatory mechanisms of DQP and its major components Salvianolic acids and Panax notoginseng saponins (DS) on lipid metabolism disorders haven’t been comprehensively studied so far. METHODS: Rat model of coronary heart disease was induced by left anterior descending (LAD) artery ligation operations. Rats were divided into sham, model, DQP treated, DS treated and positive drug (clofibrate) treated groups. At 28 days after surgery, cardiac functions were assessed by echocardiography. Expressions of transcription factors and key molecules in energy metabolism pathway were measured by reverse transcriptase polymerase chain reaction or western blotting. RESULTS: In ischemic heart model, cardiac functions were severely injured but improved by treatments of DQP and DS. Expression of LPL was down-regulated in model group. Both DQP and DS could up-regulate the mRNA expression of LPL. Membrane proteins involved in lipid transport and uptake, such as FABP4 and CPT-1A, were down-regulated in ischemic heart tissues. Treatment with DQP and DS regulated lipid metabolisms by up-regulating expressions of FABP4 and CPT-1A. DQP and DS also suppressed expression of cytochrome P450. Furthermore, transcriptional factors, such as PPARα, PPARγ, RXRA and PGC-1α, were down-regulated in ischemic model group. DQP and DS could up-regulate expressions of these factors. However, DS showed a better efficacy than DQP on PGC-1α, a coactivator of PPARs. Key molecules in signaling pathways such as AKT1/2, ERK and PI3K were also regulated by DQP and DS simultaneously. CONCLUSIONS: Salvianolic acids and Panax notoginseng are the major effective components of DanQi pill in improving lipid metabolism in ischemic heart model. The effects may be mediated by regulating transcriptional factors such as PPARs, RXRA and PGC-1α.
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spelling pubmed-51538252016-12-20 The effect of Chinese herbs and its effective components on coronary heart disease through PPARs-PGC1α pathway Wang, Qiyan Li, Chun Zhang, Qian Wang, Yuanyuan Shi, Tianjiao Lu, Linghui Zhang, Yi Wang, Yong Wang, Wei BMC Complement Altern Med Research Article BACKGROUND: DanQi pill (DQP) is prescribed widely in China and has definite cardioprotective effect on coronary heart disease. Our previous studies proved that DQP could effectively regulate plasma levels of high density lipoprotein (HDL) and low density lipoprotein (LDL). However, the regulatory mechanisms of DQP and its major components Salvianolic acids and Panax notoginseng saponins (DS) on lipid metabolism disorders haven’t been comprehensively studied so far. METHODS: Rat model of coronary heart disease was induced by left anterior descending (LAD) artery ligation operations. Rats were divided into sham, model, DQP treated, DS treated and positive drug (clofibrate) treated groups. At 28 days after surgery, cardiac functions were assessed by echocardiography. Expressions of transcription factors and key molecules in energy metabolism pathway were measured by reverse transcriptase polymerase chain reaction or western blotting. RESULTS: In ischemic heart model, cardiac functions were severely injured but improved by treatments of DQP and DS. Expression of LPL was down-regulated in model group. Both DQP and DS could up-regulate the mRNA expression of LPL. Membrane proteins involved in lipid transport and uptake, such as FABP4 and CPT-1A, were down-regulated in ischemic heart tissues. Treatment with DQP and DS regulated lipid metabolisms by up-regulating expressions of FABP4 and CPT-1A. DQP and DS also suppressed expression of cytochrome P450. Furthermore, transcriptional factors, such as PPARα, PPARγ, RXRA and PGC-1α, were down-regulated in ischemic model group. DQP and DS could up-regulate expressions of these factors. However, DS showed a better efficacy than DQP on PGC-1α, a coactivator of PPARs. Key molecules in signaling pathways such as AKT1/2, ERK and PI3K were also regulated by DQP and DS simultaneously. CONCLUSIONS: Salvianolic acids and Panax notoginseng are the major effective components of DanQi pill in improving lipid metabolism in ischemic heart model. The effects may be mediated by regulating transcriptional factors such as PPARs, RXRA and PGC-1α. BioMed Central 2016-12-12 /pmc/articles/PMC5153825/ /pubmed/27955667 http://dx.doi.org/10.1186/s12906-016-1496-z Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Wang, Qiyan
Li, Chun
Zhang, Qian
Wang, Yuanyuan
Shi, Tianjiao
Lu, Linghui
Zhang, Yi
Wang, Yong
Wang, Wei
The effect of Chinese herbs and its effective components on coronary heart disease through PPARs-PGC1α pathway
title The effect of Chinese herbs and its effective components on coronary heart disease through PPARs-PGC1α pathway
title_full The effect of Chinese herbs and its effective components on coronary heart disease through PPARs-PGC1α pathway
title_fullStr The effect of Chinese herbs and its effective components on coronary heart disease through PPARs-PGC1α pathway
title_full_unstemmed The effect of Chinese herbs and its effective components on coronary heart disease through PPARs-PGC1α pathway
title_short The effect of Chinese herbs and its effective components on coronary heart disease through PPARs-PGC1α pathway
title_sort effect of chinese herbs and its effective components on coronary heart disease through ppars-pgc1α pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5153825/
https://www.ncbi.nlm.nih.gov/pubmed/27955667
http://dx.doi.org/10.1186/s12906-016-1496-z
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