Cargando…

Functional proteomics-aided selection of protease inhibitors for herbivore insect control

Studies have reported the potential of protease inhibitors to engineer insect resistance in transgenic plants but the general usefulness of this approach in crop protection still remains to be established. Insects have evolved strategies to cope with dietary protease inhibitors, such as the use of p...

Descripción completa

Detalles Bibliográficos
Autores principales: Rasoolizadeh, Asieh, Munger, Aurélie, Goulet, Marie-Claire, Sainsbury, Frank, Cloutier, Conrad, Michaud, Dominique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5153846/
https://www.ncbi.nlm.nih.gov/pubmed/27958307
http://dx.doi.org/10.1038/srep38827
_version_ 1782474776868028416
author Rasoolizadeh, Asieh
Munger, Aurélie
Goulet, Marie-Claire
Sainsbury, Frank
Cloutier, Conrad
Michaud, Dominique
author_facet Rasoolizadeh, Asieh
Munger, Aurélie
Goulet, Marie-Claire
Sainsbury, Frank
Cloutier, Conrad
Michaud, Dominique
author_sort Rasoolizadeh, Asieh
collection PubMed
description Studies have reported the potential of protease inhibitors to engineer insect resistance in transgenic plants but the general usefulness of this approach in crop protection still remains to be established. Insects have evolved strategies to cope with dietary protease inhibitors, such as the use of proteases recalcitrant to inhibition, that often make the selection of effective inhibitors very challenging. Here, we used a functional proteomics approach for the ‘capture’ of Cys protease targets in crude protein extracts as a tool to identify promising cystatins for plant improvement. Two cystatins found to differ in their efficiency to capture Cys proteases of the coleopteran pest Leptinotarsa decemlineata also differed in their usefulness to produce transgenic potato lines resistant to this insect. Plants expressing the most potent cystatin at high level had a strong repressing effect on larval growth and leaf intake, while plants expressing the weakest cystatin showed no effect on both two parameters compared to untransformed parental line used for genetic transformation. Our data underline the relevance of considering the whole range of possible protease targets when selecting an inhibitor for plant pest control. They also confirm the feasibility of developing cystatin-expressing transgenics resistant to a major pest of potato.
format Online
Article
Text
id pubmed-5153846
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-51538462016-12-28 Functional proteomics-aided selection of protease inhibitors for herbivore insect control Rasoolizadeh, Asieh Munger, Aurélie Goulet, Marie-Claire Sainsbury, Frank Cloutier, Conrad Michaud, Dominique Sci Rep Article Studies have reported the potential of protease inhibitors to engineer insect resistance in transgenic plants but the general usefulness of this approach in crop protection still remains to be established. Insects have evolved strategies to cope with dietary protease inhibitors, such as the use of proteases recalcitrant to inhibition, that often make the selection of effective inhibitors very challenging. Here, we used a functional proteomics approach for the ‘capture’ of Cys protease targets in crude protein extracts as a tool to identify promising cystatins for plant improvement. Two cystatins found to differ in their efficiency to capture Cys proteases of the coleopteran pest Leptinotarsa decemlineata also differed in their usefulness to produce transgenic potato lines resistant to this insect. Plants expressing the most potent cystatin at high level had a strong repressing effect on larval growth and leaf intake, while plants expressing the weakest cystatin showed no effect on both two parameters compared to untransformed parental line used for genetic transformation. Our data underline the relevance of considering the whole range of possible protease targets when selecting an inhibitor for plant pest control. They also confirm the feasibility of developing cystatin-expressing transgenics resistant to a major pest of potato. Nature Publishing Group 2016-12-13 /pmc/articles/PMC5153846/ /pubmed/27958307 http://dx.doi.org/10.1038/srep38827 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Rasoolizadeh, Asieh
Munger, Aurélie
Goulet, Marie-Claire
Sainsbury, Frank
Cloutier, Conrad
Michaud, Dominique
Functional proteomics-aided selection of protease inhibitors for herbivore insect control
title Functional proteomics-aided selection of protease inhibitors for herbivore insect control
title_full Functional proteomics-aided selection of protease inhibitors for herbivore insect control
title_fullStr Functional proteomics-aided selection of protease inhibitors for herbivore insect control
title_full_unstemmed Functional proteomics-aided selection of protease inhibitors for herbivore insect control
title_short Functional proteomics-aided selection of protease inhibitors for herbivore insect control
title_sort functional proteomics-aided selection of protease inhibitors for herbivore insect control
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5153846/
https://www.ncbi.nlm.nih.gov/pubmed/27958307
http://dx.doi.org/10.1038/srep38827
work_keys_str_mv AT rasoolizadehasieh functionalproteomicsaidedselectionofproteaseinhibitorsforherbivoreinsectcontrol
AT mungeraurelie functionalproteomicsaidedselectionofproteaseinhibitorsforherbivoreinsectcontrol
AT gouletmarieclaire functionalproteomicsaidedselectionofproteaseinhibitorsforherbivoreinsectcontrol
AT sainsburyfrank functionalproteomicsaidedselectionofproteaseinhibitorsforherbivoreinsectcontrol
AT cloutierconrad functionalproteomicsaidedselectionofproteaseinhibitorsforherbivoreinsectcontrol
AT michauddominique functionalproteomicsaidedselectionofproteaseinhibitorsforherbivoreinsectcontrol