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Development of a vivo rabbit ligated intestinal Loop Model for HCMV infection

BACKGROUND: Human Cytomegalovirus (HCMV) infections can be found throughout the body, especially in epithelial tissue. Animal model was established by inoculation of HCMV (strain AD-169) or coinoculation with Hepatitis E virus (HEV) into the ligated sacculus rotundus and vermiform appendix in living...

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Autores principales: Tang, Jin, Wu, Qiaoxing, Tang, Xinming, Shi, Ruihan, Suo, Jingxia, Huang, Guangping, An, Junqing, Wang, Jingyuan, Yang, Jinling, Hao, Wenzhuo, She, Ruiping, Suo, Xun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154130/
https://www.ncbi.nlm.nih.gov/pubmed/27999668
http://dx.doi.org/10.1186/s40104-016-0129-1
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author Tang, Jin
Wu, Qiaoxing
Tang, Xinming
Shi, Ruihan
Suo, Jingxia
Huang, Guangping
An, Junqing
Wang, Jingyuan
Yang, Jinling
Hao, Wenzhuo
She, Ruiping
Suo, Xun
author_facet Tang, Jin
Wu, Qiaoxing
Tang, Xinming
Shi, Ruihan
Suo, Jingxia
Huang, Guangping
An, Junqing
Wang, Jingyuan
Yang, Jinling
Hao, Wenzhuo
She, Ruiping
Suo, Xun
author_sort Tang, Jin
collection PubMed
description BACKGROUND: Human Cytomegalovirus (HCMV) infections can be found throughout the body, especially in epithelial tissue. Animal model was established by inoculation of HCMV (strain AD-169) or coinoculation with Hepatitis E virus (HEV) into the ligated sacculus rotundus and vermiform appendix in living rabbits. The specimens were collected from animals sacrificed 1 and a half hours after infection. RESULTS: The virus was found to be capable of reproducing in these specimens through RT-PCR and Western-blot. Severe inflammation damage was found in HCMV-infected tissue. The viral protein could be detected in high amounts in the mucosal epithelium and lamina propria by immunohistochemistry and immunofluorescense. Moreover, there are strong positive signals in lymphocytes, macrophages, and lymphoid follicles. Quantitative statistics indicate that lymphocytes among epithlium cells increased significantly in viral infection groups. CONCLUSIONS: The results showed that HCMV or HEV + HCMV can efficiently infect in rabbits by vivo ligated intestine loop inoculation. The present study successfully developed an infective model in vivo rabbit ligated intestinal Loop for HCMV pathogenesis study. This rabbit model can be helpful for understanding modulation of the gut immune system with HCMV infection.
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spelling pubmed-51541302016-12-20 Development of a vivo rabbit ligated intestinal Loop Model for HCMV infection Tang, Jin Wu, Qiaoxing Tang, Xinming Shi, Ruihan Suo, Jingxia Huang, Guangping An, Junqing Wang, Jingyuan Yang, Jinling Hao, Wenzhuo She, Ruiping Suo, Xun J Anim Sci Biotechnol Methodology Article BACKGROUND: Human Cytomegalovirus (HCMV) infections can be found throughout the body, especially in epithelial tissue. Animal model was established by inoculation of HCMV (strain AD-169) or coinoculation with Hepatitis E virus (HEV) into the ligated sacculus rotundus and vermiform appendix in living rabbits. The specimens were collected from animals sacrificed 1 and a half hours after infection. RESULTS: The virus was found to be capable of reproducing in these specimens through RT-PCR and Western-blot. Severe inflammation damage was found in HCMV-infected tissue. The viral protein could be detected in high amounts in the mucosal epithelium and lamina propria by immunohistochemistry and immunofluorescense. Moreover, there are strong positive signals in lymphocytes, macrophages, and lymphoid follicles. Quantitative statistics indicate that lymphocytes among epithlium cells increased significantly in viral infection groups. CONCLUSIONS: The results showed that HCMV or HEV + HCMV can efficiently infect in rabbits by vivo ligated intestine loop inoculation. The present study successfully developed an infective model in vivo rabbit ligated intestinal Loop for HCMV pathogenesis study. This rabbit model can be helpful for understanding modulation of the gut immune system with HCMV infection. BioMed Central 2016-12-13 /pmc/articles/PMC5154130/ /pubmed/27999668 http://dx.doi.org/10.1186/s40104-016-0129-1 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Methodology Article
Tang, Jin
Wu, Qiaoxing
Tang, Xinming
Shi, Ruihan
Suo, Jingxia
Huang, Guangping
An, Junqing
Wang, Jingyuan
Yang, Jinling
Hao, Wenzhuo
She, Ruiping
Suo, Xun
Development of a vivo rabbit ligated intestinal Loop Model for HCMV infection
title Development of a vivo rabbit ligated intestinal Loop Model for HCMV infection
title_full Development of a vivo rabbit ligated intestinal Loop Model for HCMV infection
title_fullStr Development of a vivo rabbit ligated intestinal Loop Model for HCMV infection
title_full_unstemmed Development of a vivo rabbit ligated intestinal Loop Model for HCMV infection
title_short Development of a vivo rabbit ligated intestinal Loop Model for HCMV infection
title_sort development of a vivo rabbit ligated intestinal loop model for hcmv infection
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154130/
https://www.ncbi.nlm.nih.gov/pubmed/27999668
http://dx.doi.org/10.1186/s40104-016-0129-1
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