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Complete genome sequence of new bacteriophage phiE142, which causes simultaneously lysis of multidrug-resistant Escherichia coli O157:H7 and Salmonella enterica

The emergence of antibiotic-resistant foodborne bacteria is a global health problem that requires immediate attention. Bacteriophages are a promising biotechnological alternative approach against bacterial pathogens. However, a detailed analysis of phage genomes is essential to assess the safety of...

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Autores principales: Amarillas, Luis, Chaidez, Cristobal, González-Robles, Arturo, León-Félix, Josefina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154165/
https://www.ncbi.nlm.nih.gov/pubmed/27999624
http://dx.doi.org/10.1186/s40793-016-0211-5
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author Amarillas, Luis
Chaidez, Cristobal
González-Robles, Arturo
León-Félix, Josefina
author_facet Amarillas, Luis
Chaidez, Cristobal
González-Robles, Arturo
León-Félix, Josefina
author_sort Amarillas, Luis
collection PubMed
description The emergence of antibiotic-resistant foodborne bacteria is a global health problem that requires immediate attention. Bacteriophages are a promising biotechnological alternative approach against bacterial pathogens. However, a detailed analysis of phage genomes is essential to assess the safety of the phages prior to their use as biocontrol agents. Therefore, here we report the complete genome sequence of bacteriophage phiE142, which is able to lyse Salmonella and multidrug-resistant Escherichia coli O157:H7 strains. Bacteriophage phiE142 belongs to the Myoviridae family due to the presence of long non-flexible tail and icosahedral head. The genome is composed of 121,442 bp and contains 194 ORFs, and 2 tRNAs. Furthermore, the phiE142 genome does not contain any genes coding for food-borne allergens, antibiotics resistance, virulence factors, or associated with lysogenic conversion. The bacteriophage phiE142 is characterized by broad host range and compelling genetic attributes making them potential candidates as a biocontrol agent. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40793-016-0211-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-51541652016-12-20 Complete genome sequence of new bacteriophage phiE142, which causes simultaneously lysis of multidrug-resistant Escherichia coli O157:H7 and Salmonella enterica Amarillas, Luis Chaidez, Cristobal González-Robles, Arturo León-Félix, Josefina Stand Genomic Sci Short Genome Report The emergence of antibiotic-resistant foodborne bacteria is a global health problem that requires immediate attention. Bacteriophages are a promising biotechnological alternative approach against bacterial pathogens. However, a detailed analysis of phage genomes is essential to assess the safety of the phages prior to their use as biocontrol agents. Therefore, here we report the complete genome sequence of bacteriophage phiE142, which is able to lyse Salmonella and multidrug-resistant Escherichia coli O157:H7 strains. Bacteriophage phiE142 belongs to the Myoviridae family due to the presence of long non-flexible tail and icosahedral head. The genome is composed of 121,442 bp and contains 194 ORFs, and 2 tRNAs. Furthermore, the phiE142 genome does not contain any genes coding for food-borne allergens, antibiotics resistance, virulence factors, or associated with lysogenic conversion. The bacteriophage phiE142 is characterized by broad host range and compelling genetic attributes making them potential candidates as a biocontrol agent. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40793-016-0211-5) contains supplementary material, which is available to authorized users. BioMed Central 2016-12-13 /pmc/articles/PMC5154165/ /pubmed/27999624 http://dx.doi.org/10.1186/s40793-016-0211-5 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Genome Report
Amarillas, Luis
Chaidez, Cristobal
González-Robles, Arturo
León-Félix, Josefina
Complete genome sequence of new bacteriophage phiE142, which causes simultaneously lysis of multidrug-resistant Escherichia coli O157:H7 and Salmonella enterica
title Complete genome sequence of new bacteriophage phiE142, which causes simultaneously lysis of multidrug-resistant Escherichia coli O157:H7 and Salmonella enterica
title_full Complete genome sequence of new bacteriophage phiE142, which causes simultaneously lysis of multidrug-resistant Escherichia coli O157:H7 and Salmonella enterica
title_fullStr Complete genome sequence of new bacteriophage phiE142, which causes simultaneously lysis of multidrug-resistant Escherichia coli O157:H7 and Salmonella enterica
title_full_unstemmed Complete genome sequence of new bacteriophage phiE142, which causes simultaneously lysis of multidrug-resistant Escherichia coli O157:H7 and Salmonella enterica
title_short Complete genome sequence of new bacteriophage phiE142, which causes simultaneously lysis of multidrug-resistant Escherichia coli O157:H7 and Salmonella enterica
title_sort complete genome sequence of new bacteriophage phie142, which causes simultaneously lysis of multidrug-resistant escherichia coli o157:h7 and salmonella enterica
topic Short Genome Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154165/
https://www.ncbi.nlm.nih.gov/pubmed/27999624
http://dx.doi.org/10.1186/s40793-016-0211-5
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