System-specific periodicity in quantitative real-time polymerase chain reaction data questions threshold-based quantitation

Real-time quantitative polymerase chain reaction (qPCR) data are found to display periodic patterns in the fluorescence intensity as a function of sample number for fixed cycle number. This behavior is seen for technical replicate datasets recorded on several different commercial instruments; it occ...

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Detalles Bibliográficos
Autores principales: Spiess, Andrej-Nikolai, Rödiger, Stefan, Burdukiewicz, Michał, Volksdorf, Thomas, Tellinghuisen, Joel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154181/
https://www.ncbi.nlm.nih.gov/pubmed/27958340
http://dx.doi.org/10.1038/srep38951

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