Cargando…

Effect of the chemical composition of filter media on the microbial community in wastewater biofilms at different temperatures

This study investigates the microbial community composition in the biofilms grown on two different support media in fixed biofilm reactors for aerobic wastewater treatment, using next generation sequencing (NGS) technology. The chemical composition of the new type of support medium (TDR) was found t...

Descripción completa

Detalles Bibliográficos
Autores principales: Naz, Iffat, Hodgson, Douglas, Smith, Ann, Marchesi, Julian, Ahmed, Safia, Avignone-Rossa, Claudio, Saroj, Devendra P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154295/
https://www.ncbi.nlm.nih.gov/pubmed/28018581
http://dx.doi.org/10.1039/c6ra21040f
_version_ 1782474855710457856
author Naz, Iffat
Hodgson, Douglas
Smith, Ann
Marchesi, Julian
Ahmed, Safia
Avignone-Rossa, Claudio
Saroj, Devendra P.
author_facet Naz, Iffat
Hodgson, Douglas
Smith, Ann
Marchesi, Julian
Ahmed, Safia
Avignone-Rossa, Claudio
Saroj, Devendra P.
author_sort Naz, Iffat
collection PubMed
description This study investigates the microbial community composition in the biofilms grown on two different support media in fixed biofilm reactors for aerobic wastewater treatment, using next generation sequencing (NGS) technology. The chemical composition of the new type of support medium (TDR) was found to be quite different from the conventionally used support medium (stone). The analysis of 16S rRNA gene fragments recovered from the laboratory scale biofilm system show that biofilm support media and temperature conditions influence bacterial community structure and composition. Greater bacterial diversity was observed under each condition, primarily due to the large number of sequences available and sustenance of rare species. There were 6 phyla found, with the highest relative abundance shown by the phylum Proteobacteria (52.71%) followed by Bacteroidetes (33.33%), Actinobacteria (4.65%), Firmicutes, Verrucomicrobia (3.1%) and Chloroflex (>1%). The dataset showed 17 genera of bacterial populations to be commonly shared under all conditions, suggesting the presence of a core microbial community in the biofilms for wastewater treatment. However, some genera in the biofilms on TDR were observed in high proportions, which may be attributed to its chemical composition, explaining the improved level of wastewater treatment. The findings show that the structure of microbial communities in biofilm systems for wastewater treatment is affected by the properties of support matrix.
format Online
Article
Text
id pubmed-5154295
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-51542952016-12-21 Effect of the chemical composition of filter media on the microbial community in wastewater biofilms at different temperatures Naz, Iffat Hodgson, Douglas Smith, Ann Marchesi, Julian Ahmed, Safia Avignone-Rossa, Claudio Saroj, Devendra P. RSC Adv Chemistry This study investigates the microbial community composition in the biofilms grown on two different support media in fixed biofilm reactors for aerobic wastewater treatment, using next generation sequencing (NGS) technology. The chemical composition of the new type of support medium (TDR) was found to be quite different from the conventionally used support medium (stone). The analysis of 16S rRNA gene fragments recovered from the laboratory scale biofilm system show that biofilm support media and temperature conditions influence bacterial community structure and composition. Greater bacterial diversity was observed under each condition, primarily due to the large number of sequences available and sustenance of rare species. There were 6 phyla found, with the highest relative abundance shown by the phylum Proteobacteria (52.71%) followed by Bacteroidetes (33.33%), Actinobacteria (4.65%), Firmicutes, Verrucomicrobia (3.1%) and Chloroflex (>1%). The dataset showed 17 genera of bacterial populations to be commonly shared under all conditions, suggesting the presence of a core microbial community in the biofilms for wastewater treatment. However, some genera in the biofilms on TDR were observed in high proportions, which may be attributed to its chemical composition, explaining the improved level of wastewater treatment. The findings show that the structure of microbial communities in biofilm systems for wastewater treatment is affected by the properties of support matrix. Royal Society of Chemistry 2016-11-01 2016-10-26 /pmc/articles/PMC5154295/ /pubmed/28018581 http://dx.doi.org/10.1039/c6ra21040f Text en This journal is © The Royal Society of Chemistry 2016 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Naz, Iffat
Hodgson, Douglas
Smith, Ann
Marchesi, Julian
Ahmed, Safia
Avignone-Rossa, Claudio
Saroj, Devendra P.
Effect of the chemical composition of filter media on the microbial community in wastewater biofilms at different temperatures
title Effect of the chemical composition of filter media on the microbial community in wastewater biofilms at different temperatures
title_full Effect of the chemical composition of filter media on the microbial community in wastewater biofilms at different temperatures
title_fullStr Effect of the chemical composition of filter media on the microbial community in wastewater biofilms at different temperatures
title_full_unstemmed Effect of the chemical composition of filter media on the microbial community in wastewater biofilms at different temperatures
title_short Effect of the chemical composition of filter media on the microbial community in wastewater biofilms at different temperatures
title_sort effect of the chemical composition of filter media on the microbial community in wastewater biofilms at different temperatures
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154295/
https://www.ncbi.nlm.nih.gov/pubmed/28018581
http://dx.doi.org/10.1039/c6ra21040f
work_keys_str_mv AT naziffat effectofthechemicalcompositionoffiltermediaonthemicrobialcommunityinwastewaterbiofilmsatdifferenttemperatures
AT hodgsondouglas effectofthechemicalcompositionoffiltermediaonthemicrobialcommunityinwastewaterbiofilmsatdifferenttemperatures
AT smithann effectofthechemicalcompositionoffiltermediaonthemicrobialcommunityinwastewaterbiofilmsatdifferenttemperatures
AT marchesijulian effectofthechemicalcompositionoffiltermediaonthemicrobialcommunityinwastewaterbiofilmsatdifferenttemperatures
AT ahmedsafia effectofthechemicalcompositionoffiltermediaonthemicrobialcommunityinwastewaterbiofilmsatdifferenttemperatures
AT avignonerossaclaudio effectofthechemicalcompositionoffiltermediaonthemicrobialcommunityinwastewaterbiofilmsatdifferenttemperatures
AT sarojdevendrap effectofthechemicalcompositionoffiltermediaonthemicrobialcommunityinwastewaterbiofilmsatdifferenttemperatures