A genetic assay for gene essentiality in Clostridium

Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium difficile were viable only in the presence of a sta...

Descripción completa

Detalles Bibliográficos
Autores principales: Walker, David J.F., Heap, John T., Winzer, Klaus, Minton, Nigel P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2016
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154367/
https://www.ncbi.nlm.nih.gov/pubmed/27487328
http://dx.doi.org/10.1016/j.anaerobe.2016.07.007
Descripción
Sumario:Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium difficile were viable only in the presence of a stable second copy of the gene.

Ejemplares similares