A genetic assay for gene essentiality in Clostridium

Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium difficile were viable only in the presence of a sta...

Descripción completa

Detalles Bibliográficos
Autores principales: Walker, David J.F., Heap, John T., Winzer, Klaus, Minton, Nigel P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2016
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154367/
https://www.ncbi.nlm.nih.gov/pubmed/27487328
http://dx.doi.org/10.1016/j.anaerobe.2016.07.007
_version_ 1782474866379718656
author Walker, David J.F.
Heap, John T.
Winzer, Klaus
Minton, Nigel P.
author_facet Walker, David J.F.
Heap, John T.
Winzer, Klaus
Minton, Nigel P.
author_sort Walker, David J.F.
collection PubMed
description Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium difficile were viable only in the presence of a stable second copy of the gene.
format Online
Article
Text
id pubmed-5154367
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Academic Press
record_format MEDLINE/PubMed
spelling pubmed-51543672016-12-19 A genetic assay for gene essentiality in Clostridium Walker, David J.F. Heap, John T. Winzer, Klaus Minton, Nigel P. Anaerobe Short Communication Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium difficile were viable only in the presence of a stable second copy of the gene. Academic Press 2016-12 /pmc/articles/PMC5154367/ /pubmed/27487328 http://dx.doi.org/10.1016/j.anaerobe.2016.07.007 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Short Communication
Walker, David J.F.
Heap, John T.
Winzer, Klaus
Minton, Nigel P.
A genetic assay for gene essentiality in Clostridium
title A genetic assay for gene essentiality in Clostridium
title_full A genetic assay for gene essentiality in Clostridium
title_fullStr A genetic assay for gene essentiality in Clostridium
title_full_unstemmed A genetic assay for gene essentiality in Clostridium
title_short A genetic assay for gene essentiality in Clostridium
title_sort genetic assay for gene essentiality in clostridium
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154367/
https://www.ncbi.nlm.nih.gov/pubmed/27487328
http://dx.doi.org/10.1016/j.anaerobe.2016.07.007
work_keys_str_mv AT walkerdavidjf ageneticassayforgeneessentialityinclostridium
AT heapjohnt ageneticassayforgeneessentialityinclostridium
AT winzerklaus ageneticassayforgeneessentialityinclostridium
AT mintonnigelp ageneticassayforgeneessentialityinclostridium
AT walkerdavidjf geneticassayforgeneessentialityinclostridium
AT heapjohnt geneticassayforgeneessentialityinclostridium
AT winzerklaus geneticassayforgeneessentialityinclostridium
AT mintonnigelp geneticassayforgeneessentialityinclostridium

Ejemplares similares