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A genetic assay for gene essentiality in Clostridium
Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium difficile were viable only in the presence of a sta...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Academic Press
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154367/ https://www.ncbi.nlm.nih.gov/pubmed/27487328 http://dx.doi.org/10.1016/j.anaerobe.2016.07.007 |