Introduction of correlative light and airSEM(TM) microscopy imaging for tissue research under ambient conditions

A complete fingerprint of a tissue sample requires a detailed description of its cellular and extracellular components while minimizing artifacts. We introduce the application of a novel scanning electron microscope (airSEM(TM)) in conjunction with light microscopy for functional analysis of tissue...

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Autores principales: Solomonov, Inna, Talmi-Frank, Dalit, Milstein, Yonat, Addadi, Sefi, Aloshin, Anna, Sagi, Irit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154511/
https://www.ncbi.nlm.nih.gov/pubmed/25100357
http://dx.doi.org/10.1038/srep05987
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author Solomonov, Inna
Talmi-Frank, Dalit
Milstein, Yonat
Addadi, Sefi
Aloshin, Anna
Sagi, Irit
author_facet Solomonov, Inna
Talmi-Frank, Dalit
Milstein, Yonat
Addadi, Sefi
Aloshin, Anna
Sagi, Irit
author_sort Solomonov, Inna
collection PubMed
description A complete fingerprint of a tissue sample requires a detailed description of its cellular and extracellular components while minimizing artifacts. We introduce the application of a novel scanning electron microscope (airSEM(TM)) in conjunction with light microscopy for functional analysis of tissue preparations at nanometric resolution (<10 nm) and under ambient conditions. Our metal-staining protocols enable easy and detailed visualization of tissues and their extracellular scaffolds. A multimodality imaging setup, featuring airSEM(TM) and a light microscope on the same platform, provides a convenient and easy-to-use system for obtaining structural and functional correlative data. The airSEM(TM) imaging station complements other existing imaging solutions and shows great potential for studies of complex biological systems.
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spelling pubmed-51545112016-12-20 Introduction of correlative light and airSEM(TM) microscopy imaging for tissue research under ambient conditions Solomonov, Inna Talmi-Frank, Dalit Milstein, Yonat Addadi, Sefi Aloshin, Anna Sagi, Irit Sci Rep Article A complete fingerprint of a tissue sample requires a detailed description of its cellular and extracellular components while minimizing artifacts. We introduce the application of a novel scanning electron microscope (airSEM(TM)) in conjunction with light microscopy for functional analysis of tissue preparations at nanometric resolution (<10 nm) and under ambient conditions. Our metal-staining protocols enable easy and detailed visualization of tissues and their extracellular scaffolds. A multimodality imaging setup, featuring airSEM(TM) and a light microscope on the same platform, provides a convenient and easy-to-use system for obtaining structural and functional correlative data. The airSEM(TM) imaging station complements other existing imaging solutions and shows great potential for studies of complex biological systems. Nature Publishing Group 2014-08-07 /pmc/articles/PMC5154511/ /pubmed/25100357 http://dx.doi.org/10.1038/srep05987 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Solomonov, Inna
Talmi-Frank, Dalit
Milstein, Yonat
Addadi, Sefi
Aloshin, Anna
Sagi, Irit
Introduction of correlative light and airSEM(TM) microscopy imaging for tissue research under ambient conditions
title Introduction of correlative light and airSEM(TM) microscopy imaging for tissue research under ambient conditions
title_full Introduction of correlative light and airSEM(TM) microscopy imaging for tissue research under ambient conditions
title_fullStr Introduction of correlative light and airSEM(TM) microscopy imaging for tissue research under ambient conditions
title_full_unstemmed Introduction of correlative light and airSEM(TM) microscopy imaging for tissue research under ambient conditions
title_short Introduction of correlative light and airSEM(TM) microscopy imaging for tissue research under ambient conditions
title_sort introduction of correlative light and airsem(tm) microscopy imaging for tissue research under ambient conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154511/
https://www.ncbi.nlm.nih.gov/pubmed/25100357
http://dx.doi.org/10.1038/srep05987
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