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A conserved bacterial protein induces pancreatic beta cell expansion during zebrafish development

Resident microbes play important roles in the development of the gastrointestinal tract, but their influence on other digestive organs is less well explored. Using the gnotobiotic zebrafish, we discovered that the normal expansion of the pancreatic β cell population during early larval development r...

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Detalles Bibliográficos
Autores principales: Hill, Jennifer Hampton, Franzosa, Eric A, Huttenhower, Curtis, Guillemin, Karen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154760/
https://www.ncbi.nlm.nih.gov/pubmed/27960075
http://dx.doi.org/10.7554/eLife.20145
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author Hill, Jennifer Hampton
Franzosa, Eric A
Huttenhower, Curtis
Guillemin, Karen
author_facet Hill, Jennifer Hampton
Franzosa, Eric A
Huttenhower, Curtis
Guillemin, Karen
author_sort Hill, Jennifer Hampton
collection PubMed
description Resident microbes play important roles in the development of the gastrointestinal tract, but their influence on other digestive organs is less well explored. Using the gnotobiotic zebrafish, we discovered that the normal expansion of the pancreatic β cell population during early larval development requires the intestinal microbiota and that specific bacterial members can restore normal β cell numbers. These bacteria share a gene that encodes a previously undescribed protein, named herein BefA (β Cell Expansion Factor A), which is sufficient to induce β cell proliferation in developing zebrafish larvae. Homologs of BefA are present in several human-associated bacterial species, and we show that they have conserved capacity to stimulate β cell proliferation in larval zebrafish. Our findings highlight a role for the microbiota in early pancreatic β cell development and suggest a possible basis for the association between low diversity childhood fecal microbiota and increased diabetes risk. DOI: http://dx.doi.org/10.7554/eLife.20145.001
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spelling pubmed-51547602016-12-15 A conserved bacterial protein induces pancreatic beta cell expansion during zebrafish development Hill, Jennifer Hampton Franzosa, Eric A Huttenhower, Curtis Guillemin, Karen eLife Developmental Biology and Stem Cells Resident microbes play important roles in the development of the gastrointestinal tract, but their influence on other digestive organs is less well explored. Using the gnotobiotic zebrafish, we discovered that the normal expansion of the pancreatic β cell population during early larval development requires the intestinal microbiota and that specific bacterial members can restore normal β cell numbers. These bacteria share a gene that encodes a previously undescribed protein, named herein BefA (β Cell Expansion Factor A), which is sufficient to induce β cell proliferation in developing zebrafish larvae. Homologs of BefA are present in several human-associated bacterial species, and we show that they have conserved capacity to stimulate β cell proliferation in larval zebrafish. Our findings highlight a role for the microbiota in early pancreatic β cell development and suggest a possible basis for the association between low diversity childhood fecal microbiota and increased diabetes risk. DOI: http://dx.doi.org/10.7554/eLife.20145.001 eLife Sciences Publications, Ltd 2016-12-13 /pmc/articles/PMC5154760/ /pubmed/27960075 http://dx.doi.org/10.7554/eLife.20145 Text en © 2016, Hill et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology and Stem Cells
Hill, Jennifer Hampton
Franzosa, Eric A
Huttenhower, Curtis
Guillemin, Karen
A conserved bacterial protein induces pancreatic beta cell expansion during zebrafish development
title A conserved bacterial protein induces pancreatic beta cell expansion during zebrafish development
title_full A conserved bacterial protein induces pancreatic beta cell expansion during zebrafish development
title_fullStr A conserved bacterial protein induces pancreatic beta cell expansion during zebrafish development
title_full_unstemmed A conserved bacterial protein induces pancreatic beta cell expansion during zebrafish development
title_short A conserved bacterial protein induces pancreatic beta cell expansion during zebrafish development
title_sort conserved bacterial protein induces pancreatic beta cell expansion during zebrafish development
topic Developmental Biology and Stem Cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154760/
https://www.ncbi.nlm.nih.gov/pubmed/27960075
http://dx.doi.org/10.7554/eLife.20145
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