Cargando…

Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity

Psoriasis is a chronic and recurrent dermatitis, mediated by keratinocytes and T cells. Several proinflammatory cytokines contribute to formation and maintenance of psoriatic plaque. The Th1/Th17 pathways and some of IL-1 family members were involved in psoriasis pathogenesis and could contribute to...

Descripción completa

Detalles Bibliográficos
Autores principales: de Oliveira, Priscilla Stela Santana, Pereira, Michelly Cristiny, Silva de Paula, Simão Kalebe, Lima, Emerson Vasconcelos Andrade, Lima, Mariana Modesto de Andrade, de Arruda, Rodrigo Gomes, de Oliveira, Wagner Luís Mendes, Duarte, Ângela Luzia Branco Pinto, Pitta, Ivan da Rocha, Rêgo, Moacyr Jesus Melo Barreto, Galdino da Rocha Pitta, Maira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155088/
https://www.ncbi.nlm.nih.gov/pubmed/28042206
http://dx.doi.org/10.1155/2016/4395276
Descripción
Sumario:Psoriasis is a chronic and recurrent dermatitis, mediated by keratinocytes and T cells. Several proinflammatory cytokines contribute to formation and maintenance of psoriatic plaque. The Th1/Th17 pathways and some of IL-1 family members were involved in psoriasis pathogenesis and could contribute to disease activity. Therefore, we sought to analyse skin transcript levels of IL17A, IL22, RORC, IL8, IFNG, IL33, IL36A, FOXP3, and IL10 and correlate with clinic of patients with plaque-type psoriasis. In order to conduct that, we collected punch biopsies from lesional skin and obtained tissue RNA. After reverse transcription, qRT-PCR quantified the relative mRNA expression. The main results revealed increased transcripts levels of IL17A, IFNG, and FOXP3 in moderate-severe patients. Despite this, only IL17A can increase the chance to worsen disease severity. We also observed many significant positive correlations between each transcript. In conclusion, IL17A is elevated in lesional skin from psoriasis patients and plays crucial role in disease severity.