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Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity

Psoriasis is a chronic and recurrent dermatitis, mediated by keratinocytes and T cells. Several proinflammatory cytokines contribute to formation and maintenance of psoriatic plaque. The Th1/Th17 pathways and some of IL-1 family members were involved in psoriasis pathogenesis and could contribute to...

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Autores principales: de Oliveira, Priscilla Stela Santana, Pereira, Michelly Cristiny, Silva de Paula, Simão Kalebe, Lima, Emerson Vasconcelos Andrade, Lima, Mariana Modesto de Andrade, de Arruda, Rodrigo Gomes, de Oliveira, Wagner Luís Mendes, Duarte, Ângela Luzia Branco Pinto, Pitta, Ivan da Rocha, Rêgo, Moacyr Jesus Melo Barreto, Galdino da Rocha Pitta, Maira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155088/
https://www.ncbi.nlm.nih.gov/pubmed/28042206
http://dx.doi.org/10.1155/2016/4395276
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author de Oliveira, Priscilla Stela Santana
Pereira, Michelly Cristiny
Silva de Paula, Simão Kalebe
Lima, Emerson Vasconcelos Andrade
Lima, Mariana Modesto de Andrade
de Arruda, Rodrigo Gomes
de Oliveira, Wagner Luís Mendes
Duarte, Ângela Luzia Branco Pinto
Pitta, Ivan da Rocha
Rêgo, Moacyr Jesus Melo Barreto
Galdino da Rocha Pitta, Maira
author_facet de Oliveira, Priscilla Stela Santana
Pereira, Michelly Cristiny
Silva de Paula, Simão Kalebe
Lima, Emerson Vasconcelos Andrade
Lima, Mariana Modesto de Andrade
de Arruda, Rodrigo Gomes
de Oliveira, Wagner Luís Mendes
Duarte, Ângela Luzia Branco Pinto
Pitta, Ivan da Rocha
Rêgo, Moacyr Jesus Melo Barreto
Galdino da Rocha Pitta, Maira
author_sort de Oliveira, Priscilla Stela Santana
collection PubMed
description Psoriasis is a chronic and recurrent dermatitis, mediated by keratinocytes and T cells. Several proinflammatory cytokines contribute to formation and maintenance of psoriatic plaque. The Th1/Th17 pathways and some of IL-1 family members were involved in psoriasis pathogenesis and could contribute to disease activity. Therefore, we sought to analyse skin transcript levels of IL17A, IL22, RORC, IL8, IFNG, IL33, IL36A, FOXP3, and IL10 and correlate with clinic of patients with plaque-type psoriasis. In order to conduct that, we collected punch biopsies from lesional skin and obtained tissue RNA. After reverse transcription, qRT-PCR quantified the relative mRNA expression. The main results revealed increased transcripts levels of IL17A, IFNG, and FOXP3 in moderate-severe patients. Despite this, only IL17A can increase the chance to worsen disease severity. We also observed many significant positive correlations between each transcript. In conclusion, IL17A is elevated in lesional skin from psoriasis patients and plays crucial role in disease severity.
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spelling pubmed-51550882017-01-01 Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity de Oliveira, Priscilla Stela Santana Pereira, Michelly Cristiny Silva de Paula, Simão Kalebe Lima, Emerson Vasconcelos Andrade Lima, Mariana Modesto de Andrade de Arruda, Rodrigo Gomes de Oliveira, Wagner Luís Mendes Duarte, Ângela Luzia Branco Pinto Pitta, Ivan da Rocha Rêgo, Moacyr Jesus Melo Barreto Galdino da Rocha Pitta, Maira Mediators Inflamm Research Article Psoriasis is a chronic and recurrent dermatitis, mediated by keratinocytes and T cells. Several proinflammatory cytokines contribute to formation and maintenance of psoriatic plaque. The Th1/Th17 pathways and some of IL-1 family members were involved in psoriasis pathogenesis and could contribute to disease activity. Therefore, we sought to analyse skin transcript levels of IL17A, IL22, RORC, IL8, IFNG, IL33, IL36A, FOXP3, and IL10 and correlate with clinic of patients with plaque-type psoriasis. In order to conduct that, we collected punch biopsies from lesional skin and obtained tissue RNA. After reverse transcription, qRT-PCR quantified the relative mRNA expression. The main results revealed increased transcripts levels of IL17A, IFNG, and FOXP3 in moderate-severe patients. Despite this, only IL17A can increase the chance to worsen disease severity. We also observed many significant positive correlations between each transcript. In conclusion, IL17A is elevated in lesional skin from psoriasis patients and plays crucial role in disease severity. Hindawi Publishing Corporation 2016 2016-11-30 /pmc/articles/PMC5155088/ /pubmed/28042206 http://dx.doi.org/10.1155/2016/4395276 Text en Copyright © 2016 Priscilla Stela Santana de Oliveira et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
de Oliveira, Priscilla Stela Santana
Pereira, Michelly Cristiny
Silva de Paula, Simão Kalebe
Lima, Emerson Vasconcelos Andrade
Lima, Mariana Modesto de Andrade
de Arruda, Rodrigo Gomes
de Oliveira, Wagner Luís Mendes
Duarte, Ângela Luzia Branco Pinto
Pitta, Ivan da Rocha
Rêgo, Moacyr Jesus Melo Barreto
Galdino da Rocha Pitta, Maira
Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity
title Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity
title_full Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity
title_fullStr Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity
title_full_unstemmed Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity
title_short Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity
title_sort increased il17a, ifng, and foxp3 transcripts in moderate-severe psoriasis: a major influence exerted by il17a in disease severity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155088/
https://www.ncbi.nlm.nih.gov/pubmed/28042206
http://dx.doi.org/10.1155/2016/4395276
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