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Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity
Psoriasis is a chronic and recurrent dermatitis, mediated by keratinocytes and T cells. Several proinflammatory cytokines contribute to formation and maintenance of psoriatic plaque. The Th1/Th17 pathways and some of IL-1 family members were involved in psoriasis pathogenesis and could contribute to...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155088/ https://www.ncbi.nlm.nih.gov/pubmed/28042206 http://dx.doi.org/10.1155/2016/4395276 |
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author | de Oliveira, Priscilla Stela Santana Pereira, Michelly Cristiny Silva de Paula, Simão Kalebe Lima, Emerson Vasconcelos Andrade Lima, Mariana Modesto de Andrade de Arruda, Rodrigo Gomes de Oliveira, Wagner Luís Mendes Duarte, Ângela Luzia Branco Pinto Pitta, Ivan da Rocha Rêgo, Moacyr Jesus Melo Barreto Galdino da Rocha Pitta, Maira |
author_facet | de Oliveira, Priscilla Stela Santana Pereira, Michelly Cristiny Silva de Paula, Simão Kalebe Lima, Emerson Vasconcelos Andrade Lima, Mariana Modesto de Andrade de Arruda, Rodrigo Gomes de Oliveira, Wagner Luís Mendes Duarte, Ângela Luzia Branco Pinto Pitta, Ivan da Rocha Rêgo, Moacyr Jesus Melo Barreto Galdino da Rocha Pitta, Maira |
author_sort | de Oliveira, Priscilla Stela Santana |
collection | PubMed |
description | Psoriasis is a chronic and recurrent dermatitis, mediated by keratinocytes and T cells. Several proinflammatory cytokines contribute to formation and maintenance of psoriatic plaque. The Th1/Th17 pathways and some of IL-1 family members were involved in psoriasis pathogenesis and could contribute to disease activity. Therefore, we sought to analyse skin transcript levels of IL17A, IL22, RORC, IL8, IFNG, IL33, IL36A, FOXP3, and IL10 and correlate with clinic of patients with plaque-type psoriasis. In order to conduct that, we collected punch biopsies from lesional skin and obtained tissue RNA. After reverse transcription, qRT-PCR quantified the relative mRNA expression. The main results revealed increased transcripts levels of IL17A, IFNG, and FOXP3 in moderate-severe patients. Despite this, only IL17A can increase the chance to worsen disease severity. We also observed many significant positive correlations between each transcript. In conclusion, IL17A is elevated in lesional skin from psoriasis patients and plays crucial role in disease severity. |
format | Online Article Text |
id | pubmed-5155088 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-51550882017-01-01 Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity de Oliveira, Priscilla Stela Santana Pereira, Michelly Cristiny Silva de Paula, Simão Kalebe Lima, Emerson Vasconcelos Andrade Lima, Mariana Modesto de Andrade de Arruda, Rodrigo Gomes de Oliveira, Wagner Luís Mendes Duarte, Ângela Luzia Branco Pinto Pitta, Ivan da Rocha Rêgo, Moacyr Jesus Melo Barreto Galdino da Rocha Pitta, Maira Mediators Inflamm Research Article Psoriasis is a chronic and recurrent dermatitis, mediated by keratinocytes and T cells. Several proinflammatory cytokines contribute to formation and maintenance of psoriatic plaque. The Th1/Th17 pathways and some of IL-1 family members were involved in psoriasis pathogenesis and could contribute to disease activity. Therefore, we sought to analyse skin transcript levels of IL17A, IL22, RORC, IL8, IFNG, IL33, IL36A, FOXP3, and IL10 and correlate with clinic of patients with plaque-type psoriasis. In order to conduct that, we collected punch biopsies from lesional skin and obtained tissue RNA. After reverse transcription, qRT-PCR quantified the relative mRNA expression. The main results revealed increased transcripts levels of IL17A, IFNG, and FOXP3 in moderate-severe patients. Despite this, only IL17A can increase the chance to worsen disease severity. We also observed many significant positive correlations between each transcript. In conclusion, IL17A is elevated in lesional skin from psoriasis patients and plays crucial role in disease severity. Hindawi Publishing Corporation 2016 2016-11-30 /pmc/articles/PMC5155088/ /pubmed/28042206 http://dx.doi.org/10.1155/2016/4395276 Text en Copyright © 2016 Priscilla Stela Santana de Oliveira et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article de Oliveira, Priscilla Stela Santana Pereira, Michelly Cristiny Silva de Paula, Simão Kalebe Lima, Emerson Vasconcelos Andrade Lima, Mariana Modesto de Andrade de Arruda, Rodrigo Gomes de Oliveira, Wagner Luís Mendes Duarte, Ângela Luzia Branco Pinto Pitta, Ivan da Rocha Rêgo, Moacyr Jesus Melo Barreto Galdino da Rocha Pitta, Maira Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity |
title | Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity |
title_full | Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity |
title_fullStr | Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity |
title_full_unstemmed | Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity |
title_short | Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity |
title_sort | increased il17a, ifng, and foxp3 transcripts in moderate-severe psoriasis: a major influence exerted by il17a in disease severity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155088/ https://www.ncbi.nlm.nih.gov/pubmed/28042206 http://dx.doi.org/10.1155/2016/4395276 |
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