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Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application

Limbal stem cells (LSC) are the progenitor cells that maintain the transparency of the cornea. Limbal stem cell deficiency (LSCD) leads to corneal opacity, inflammation, scarring, and blindness. A clinical approach to treat this condition consists in LSC transplantation (LSCT) after ex vivo expansio...

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Autores principales: Nieto-Nicolau, Nuria, Martínez-Conesa, Eva M., Casaroli-Marano, Ricardo P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155095/
https://www.ncbi.nlm.nih.gov/pubmed/28042298
http://dx.doi.org/10.1155/2016/3032128
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author Nieto-Nicolau, Nuria
Martínez-Conesa, Eva M.
Casaroli-Marano, Ricardo P.
author_facet Nieto-Nicolau, Nuria
Martínez-Conesa, Eva M.
Casaroli-Marano, Ricardo P.
author_sort Nieto-Nicolau, Nuria
collection PubMed
description Limbal stem cells (LSC) are the progenitor cells that maintain the transparency of the cornea. Limbal stem cell deficiency (LSCD) leads to corneal opacity, inflammation, scarring, and blindness. A clinical approach to treat this condition consists in LSC transplantation (LSCT) after ex vivo expansion of LSC. In unilateral LSCD, an autologous transplant is possible, but cases of bilateral LSCD require allogenic LSCT. Cadaveric donors represent the most important source of LSC allografts for treatment of bilateral LSCD when living relative donors are not available. To evaluate the suitability of aged cadaveric donors for LSCT, we compared three pools of LSC from donors of different ages (<60 years, 60–75 years, and >75 years). We evaluated graft quality in terms of percent of p63-positive (p63+) cells by immunofluorescence, colony forming efficiency, and mRNA and protein expression of p63, PAX6, Wnt7a, E-cadherin, and cytokeratin (CK) 12, CK3, and CK19. The results showed that LSC cultures from aged donors can express ≥3% of p63+ cells—considered as the minimum value for predicting favorable clinical outcomes after LSCT—suggesting that these cells could be a suitable source of LSC for transplantation. Our results also indicate the need to evaluate LSC graft quality criteria for each donor.
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spelling pubmed-51550952017-01-01 Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application Nieto-Nicolau, Nuria Martínez-Conesa, Eva M. Casaroli-Marano, Ricardo P. Stem Cells Int Research Article Limbal stem cells (LSC) are the progenitor cells that maintain the transparency of the cornea. Limbal stem cell deficiency (LSCD) leads to corneal opacity, inflammation, scarring, and blindness. A clinical approach to treat this condition consists in LSC transplantation (LSCT) after ex vivo expansion of LSC. In unilateral LSCD, an autologous transplant is possible, but cases of bilateral LSCD require allogenic LSCT. Cadaveric donors represent the most important source of LSC allografts for treatment of bilateral LSCD when living relative donors are not available. To evaluate the suitability of aged cadaveric donors for LSCT, we compared three pools of LSC from donors of different ages (<60 years, 60–75 years, and >75 years). We evaluated graft quality in terms of percent of p63-positive (p63+) cells by immunofluorescence, colony forming efficiency, and mRNA and protein expression of p63, PAX6, Wnt7a, E-cadherin, and cytokeratin (CK) 12, CK3, and CK19. The results showed that LSC cultures from aged donors can express ≥3% of p63+ cells—considered as the minimum value for predicting favorable clinical outcomes after LSCT—suggesting that these cells could be a suitable source of LSC for transplantation. Our results also indicate the need to evaluate LSC graft quality criteria for each donor. Hindawi Publishing Corporation 2016 2016-11-30 /pmc/articles/PMC5155095/ /pubmed/28042298 http://dx.doi.org/10.1155/2016/3032128 Text en Copyright © 2016 Nuria Nieto-Nicolau et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nieto-Nicolau, Nuria
Martínez-Conesa, Eva M.
Casaroli-Marano, Ricardo P.
Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application
title Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application
title_full Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application
title_fullStr Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application
title_full_unstemmed Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application
title_short Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application
title_sort limbal stem cells from aged donors are a suitable source for clinical application
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155095/
https://www.ncbi.nlm.nih.gov/pubmed/28042298
http://dx.doi.org/10.1155/2016/3032128
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