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Characterization of smooth muscle, enteric nerve, interstitial cells of Cajal, and fibroblast-like cells in the gastric musculature of patients with diabetes mellitus

AIM: To investigate histologic abnormalities in the gastric smooth muscle of patients with diabetes mellitus (DM). METHODS: Full-thickness gastric specimens were obtained from patients undergoing surgery for gastric cancer. H&E stain and Masson’s Trichrome stain were performed to assess the degr...

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Autores principales: Park, Kyung Sik, Cho, Kwang Bum, Hwang, Il Seon, Park, Jae Hyung, Jang, Byung Ik, Kim, Kyeong Ok, Jeon, Sung Woo, Kim, Eun Soo, Park, Chang Sik, Kwon, Joong Goo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155172/
https://www.ncbi.nlm.nih.gov/pubmed/28028361
http://dx.doi.org/10.3748/wjg.v22.i46.10131
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author Park, Kyung Sik
Cho, Kwang Bum
Hwang, Il Seon
Park, Jae Hyung
Jang, Byung Ik
Kim, Kyeong Ok
Jeon, Sung Woo
Kim, Eun Soo
Park, Chang Sik
Kwon, Joong Goo
author_facet Park, Kyung Sik
Cho, Kwang Bum
Hwang, Il Seon
Park, Jae Hyung
Jang, Byung Ik
Kim, Kyeong Ok
Jeon, Sung Woo
Kim, Eun Soo
Park, Chang Sik
Kwon, Joong Goo
author_sort Park, Kyung Sik
collection PubMed
description AIM: To investigate histologic abnormalities in the gastric smooth muscle of patients with diabetes mellitus (DM). METHODS: Full-thickness gastric specimens were obtained from patients undergoing surgery for gastric cancer. H&E stain and Masson’s Trichrome stain were performed to assess the degree of fibrosis. Immunohistochemical staining using various antibodies was also performed [antibodies against protein gene product 9.5 (PGP9.5), neuronal nitric oxide synthase (nNOS), vasoactive intestinal peptide (VIP), neurokinin-1 (NK1) receptor, c-Kit, and platelet-derived growth factor receptor-alpha, (PDGFRα)]. Immunofluorescent staining and evaluation with confocal microscopy were also conducted. RESULTS: Twenty-six controls and 35 diabetic patients (21 short-duration patients and 14 long-duration patients) were included. There were no significant differences in basic demographics between the two groups except in mean body mass index (BMI) (higher in the DM group). Proportions of moderate-to-severe intercellular fibrosis in the muscle layer were significantly higher in the DM group than in the control group (P < 0.01). On immunohistochemical staining, c-Kit- and PDGFRα-positive immunoreactivity were significantly decreased in the DM group compared with the control group (P < 0.05). There were no statistically significant differences in PGP9.5, nNOS, VIP, and neurokinin 1 expression. On immunofluorescent staining, cellularity of interstitial cells of Cajal (ICC) was observed to decrease with increasing duration of DM. CONCLUSION: Our study suggests that increased intercellular fibrosis, loss of ICC, and loss of fibroblast-like cells are found in the smooth muscle of DM patients. These abnormalities may contribute to changes in gastric motor activity in patients with DM.
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spelling pubmed-51551722016-12-27 Characterization of smooth muscle, enteric nerve, interstitial cells of Cajal, and fibroblast-like cells in the gastric musculature of patients with diabetes mellitus Park, Kyung Sik Cho, Kwang Bum Hwang, Il Seon Park, Jae Hyung Jang, Byung Ik Kim, Kyeong Ok Jeon, Sung Woo Kim, Eun Soo Park, Chang Sik Kwon, Joong Goo World J Gastroenterol Basic Study AIM: To investigate histologic abnormalities in the gastric smooth muscle of patients with diabetes mellitus (DM). METHODS: Full-thickness gastric specimens were obtained from patients undergoing surgery for gastric cancer. H&E stain and Masson’s Trichrome stain were performed to assess the degree of fibrosis. Immunohistochemical staining using various antibodies was also performed [antibodies against protein gene product 9.5 (PGP9.5), neuronal nitric oxide synthase (nNOS), vasoactive intestinal peptide (VIP), neurokinin-1 (NK1) receptor, c-Kit, and platelet-derived growth factor receptor-alpha, (PDGFRα)]. Immunofluorescent staining and evaluation with confocal microscopy were also conducted. RESULTS: Twenty-six controls and 35 diabetic patients (21 short-duration patients and 14 long-duration patients) were included. There were no significant differences in basic demographics between the two groups except in mean body mass index (BMI) (higher in the DM group). Proportions of moderate-to-severe intercellular fibrosis in the muscle layer were significantly higher in the DM group than in the control group (P < 0.01). On immunohistochemical staining, c-Kit- and PDGFRα-positive immunoreactivity were significantly decreased in the DM group compared with the control group (P < 0.05). There were no statistically significant differences in PGP9.5, nNOS, VIP, and neurokinin 1 expression. On immunofluorescent staining, cellularity of interstitial cells of Cajal (ICC) was observed to decrease with increasing duration of DM. CONCLUSION: Our study suggests that increased intercellular fibrosis, loss of ICC, and loss of fibroblast-like cells are found in the smooth muscle of DM patients. These abnormalities may contribute to changes in gastric motor activity in patients with DM. Baishideng Publishing Group Inc 2016-12-14 2016-12-14 /pmc/articles/PMC5155172/ /pubmed/28028361 http://dx.doi.org/10.3748/wjg.v22.i46.10131 Text en ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.
spellingShingle Basic Study
Park, Kyung Sik
Cho, Kwang Bum
Hwang, Il Seon
Park, Jae Hyung
Jang, Byung Ik
Kim, Kyeong Ok
Jeon, Sung Woo
Kim, Eun Soo
Park, Chang Sik
Kwon, Joong Goo
Characterization of smooth muscle, enteric nerve, interstitial cells of Cajal, and fibroblast-like cells in the gastric musculature of patients with diabetes mellitus
title Characterization of smooth muscle, enteric nerve, interstitial cells of Cajal, and fibroblast-like cells in the gastric musculature of patients with diabetes mellitus
title_full Characterization of smooth muscle, enteric nerve, interstitial cells of Cajal, and fibroblast-like cells in the gastric musculature of patients with diabetes mellitus
title_fullStr Characterization of smooth muscle, enteric nerve, interstitial cells of Cajal, and fibroblast-like cells in the gastric musculature of patients with diabetes mellitus
title_full_unstemmed Characterization of smooth muscle, enteric nerve, interstitial cells of Cajal, and fibroblast-like cells in the gastric musculature of patients with diabetes mellitus
title_short Characterization of smooth muscle, enteric nerve, interstitial cells of Cajal, and fibroblast-like cells in the gastric musculature of patients with diabetes mellitus
title_sort characterization of smooth muscle, enteric nerve, interstitial cells of cajal, and fibroblast-like cells in the gastric musculature of patients with diabetes mellitus
topic Basic Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155172/
https://www.ncbi.nlm.nih.gov/pubmed/28028361
http://dx.doi.org/10.3748/wjg.v22.i46.10131
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