Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples

The zebra mussel (Dreissena polymorpha Pallas, 1771) and the quagga mussel (D. rostriformis Deshayes, 1838) are successful invasive bivalves with substantial ecological and economic impacts in freshwater systems once they become established. Since their eradication is extremely difficult, their dete...

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Autores principales: Peñarrubia, Luis, Alcaraz, Carles, Vaate, Abraham bij de, Sanz, Nuria, Pla, Carles, Vidal, Oriol, Viñas, Jordi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155230/
https://www.ncbi.nlm.nih.gov/pubmed/27966602
http://dx.doi.org/10.1038/srep39067
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author Peñarrubia, Luis
Alcaraz, Carles
Vaate, Abraham bij de
Sanz, Nuria
Pla, Carles
Vidal, Oriol
Viñas, Jordi
author_facet Peñarrubia, Luis
Alcaraz, Carles
Vaate, Abraham bij de
Sanz, Nuria
Pla, Carles
Vidal, Oriol
Viñas, Jordi
author_sort Peñarrubia, Luis
collection PubMed
description The zebra mussel (Dreissena polymorpha Pallas, 1771) and the quagga mussel (D. rostriformis Deshayes, 1838) are successful invasive bivalves with substantial ecological and economic impacts in freshwater systems once they become established. Since their eradication is extremely difficult, their detection at an early stage is crucial to prevent spread. In this study, we optimized and validated a qPCR detection method based on the histone H2B gene to quantify combined infestation levels of zebra and quagga mussels in environmental DNA samples. Our results show specific dreissenid DNA present in filtered water samples for which microscopic diagnostic identification for larvae failed. Monitoring a large number of locations for invasive dreissenid species based on a highly specific environmental DNA qPCR assay may prove to be an essential tool for management and control plans focused on prevention of establishment of dreissenid mussels in new locations.
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spelling pubmed-51552302016-12-28 Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples Peñarrubia, Luis Alcaraz, Carles Vaate, Abraham bij de Sanz, Nuria Pla, Carles Vidal, Oriol Viñas, Jordi Sci Rep Article The zebra mussel (Dreissena polymorpha Pallas, 1771) and the quagga mussel (D. rostriformis Deshayes, 1838) are successful invasive bivalves with substantial ecological and economic impacts in freshwater systems once they become established. Since their eradication is extremely difficult, their detection at an early stage is crucial to prevent spread. In this study, we optimized and validated a qPCR detection method based on the histone H2B gene to quantify combined infestation levels of zebra and quagga mussels in environmental DNA samples. Our results show specific dreissenid DNA present in filtered water samples for which microscopic diagnostic identification for larvae failed. Monitoring a large number of locations for invasive dreissenid species based on a highly specific environmental DNA qPCR assay may prove to be an essential tool for management and control plans focused on prevention of establishment of dreissenid mussels in new locations. Nature Publishing Group 2016-12-14 /pmc/articles/PMC5155230/ /pubmed/27966602 http://dx.doi.org/10.1038/srep39067 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Peñarrubia, Luis
Alcaraz, Carles
Vaate, Abraham bij de
Sanz, Nuria
Pla, Carles
Vidal, Oriol
Viñas, Jordi
Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples
title Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples
title_full Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples
title_fullStr Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples
title_full_unstemmed Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples
title_short Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples
title_sort validated methodology for quantifying infestation levels of dreissenid mussels in environmental dna (edna) samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155230/
https://www.ncbi.nlm.nih.gov/pubmed/27966602
http://dx.doi.org/10.1038/srep39067
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