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Host cell surfaces induce a Type IV pili-dependent alteration of bacterial swimming
For most pathogenic bacteria, flagellar motility is recognized as a virulence factor. Here, we analysed the swimming behaviour of bacteria close to eukaryotic cellular surfaces, using the major opportunistic pathogen Pseudomonas aeruginosa as a model. We delineated three classes of swimming trajecto...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155295/ https://www.ncbi.nlm.nih.gov/pubmed/27966607 http://dx.doi.org/10.1038/srep38950 |
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author | Golovkine, Guillaume Lemelle, Laurence Burny, Claire Vaillant, Cedric Palierne, Jean-Francois Place, Christophe Huber, Philippe |
author_facet | Golovkine, Guillaume Lemelle, Laurence Burny, Claire Vaillant, Cedric Palierne, Jean-Francois Place, Christophe Huber, Philippe |
author_sort | Golovkine, Guillaume |
collection | PubMed |
description | For most pathogenic bacteria, flagellar motility is recognized as a virulence factor. Here, we analysed the swimming behaviour of bacteria close to eukaryotic cellular surfaces, using the major opportunistic pathogen Pseudomonas aeruginosa as a model. We delineated three classes of swimming trajectories on both cellular surfaces and glass that could be differentiated by their speeds and local curvatures, resulting from different levels of hydrodynamic interactions with the surface. Segmentation of the trajectories into linear and curved sections or pause allowed us to precisely describe the corresponding swimming patterns near the two surfaces. We concluded that (i) the trajectory classes were of same nature on cells and glass, however the trajectory distribution was strikingly different between surface types, (ii) on cell monolayers, a larger fraction of bacteria adopted a swimming mode with stronger bacteria-surface interaction mostly dependent upon Type IV pili. Thus, bacteria swim near boundaries with diverse patterns and importantly, Type IV pili differentially influence swimming near cellular and abiotic surfaces. |
format | Online Article Text |
id | pubmed-5155295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-51552952016-12-20 Host cell surfaces induce a Type IV pili-dependent alteration of bacterial swimming Golovkine, Guillaume Lemelle, Laurence Burny, Claire Vaillant, Cedric Palierne, Jean-Francois Place, Christophe Huber, Philippe Sci Rep Article For most pathogenic bacteria, flagellar motility is recognized as a virulence factor. Here, we analysed the swimming behaviour of bacteria close to eukaryotic cellular surfaces, using the major opportunistic pathogen Pseudomonas aeruginosa as a model. We delineated three classes of swimming trajectories on both cellular surfaces and glass that could be differentiated by their speeds and local curvatures, resulting from different levels of hydrodynamic interactions with the surface. Segmentation of the trajectories into linear and curved sections or pause allowed us to precisely describe the corresponding swimming patterns near the two surfaces. We concluded that (i) the trajectory classes were of same nature on cells and glass, however the trajectory distribution was strikingly different between surface types, (ii) on cell monolayers, a larger fraction of bacteria adopted a swimming mode with stronger bacteria-surface interaction mostly dependent upon Type IV pili. Thus, bacteria swim near boundaries with diverse patterns and importantly, Type IV pili differentially influence swimming near cellular and abiotic surfaces. Nature Publishing Group 2016-12-14 /pmc/articles/PMC5155295/ /pubmed/27966607 http://dx.doi.org/10.1038/srep38950 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Golovkine, Guillaume Lemelle, Laurence Burny, Claire Vaillant, Cedric Palierne, Jean-Francois Place, Christophe Huber, Philippe Host cell surfaces induce a Type IV pili-dependent alteration of bacterial swimming |
title | Host cell surfaces induce a Type IV pili-dependent alteration of bacterial swimming |
title_full | Host cell surfaces induce a Type IV pili-dependent alteration of bacterial swimming |
title_fullStr | Host cell surfaces induce a Type IV pili-dependent alteration of bacterial swimming |
title_full_unstemmed | Host cell surfaces induce a Type IV pili-dependent alteration of bacterial swimming |
title_short | Host cell surfaces induce a Type IV pili-dependent alteration of bacterial swimming |
title_sort | host cell surfaces induce a type iv pili-dependent alteration of bacterial swimming |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155295/ https://www.ncbi.nlm.nih.gov/pubmed/27966607 http://dx.doi.org/10.1038/srep38950 |
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