Cargando…

Host cell surfaces induce a Type IV pili-dependent alteration of bacterial swimming

For most pathogenic bacteria, flagellar motility is recognized as a virulence factor. Here, we analysed the swimming behaviour of bacteria close to eukaryotic cellular surfaces, using the major opportunistic pathogen Pseudomonas aeruginosa as a model. We delineated three classes of swimming trajecto...

Descripción completa

Detalles Bibliográficos
Autores principales: Golovkine, Guillaume, Lemelle, Laurence, Burny, Claire, Vaillant, Cedric, Palierne, Jean-Francois, Place, Christophe, Huber, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155295/
https://www.ncbi.nlm.nih.gov/pubmed/27966607
http://dx.doi.org/10.1038/srep38950
_version_ 1782474979551477760
author Golovkine, Guillaume
Lemelle, Laurence
Burny, Claire
Vaillant, Cedric
Palierne, Jean-Francois
Place, Christophe
Huber, Philippe
author_facet Golovkine, Guillaume
Lemelle, Laurence
Burny, Claire
Vaillant, Cedric
Palierne, Jean-Francois
Place, Christophe
Huber, Philippe
author_sort Golovkine, Guillaume
collection PubMed
description For most pathogenic bacteria, flagellar motility is recognized as a virulence factor. Here, we analysed the swimming behaviour of bacteria close to eukaryotic cellular surfaces, using the major opportunistic pathogen Pseudomonas aeruginosa as a model. We delineated three classes of swimming trajectories on both cellular surfaces and glass that could be differentiated by their speeds and local curvatures, resulting from different levels of hydrodynamic interactions with the surface. Segmentation of the trajectories into linear and curved sections or pause allowed us to precisely describe the corresponding swimming patterns near the two surfaces. We concluded that (i) the trajectory classes were of same nature on cells and glass, however the trajectory distribution was strikingly different between surface types, (ii) on cell monolayers, a larger fraction of bacteria adopted a swimming mode with stronger bacteria-surface interaction mostly dependent upon Type IV pili. Thus, bacteria swim near boundaries with diverse patterns and importantly, Type IV pili differentially influence swimming near cellular and abiotic surfaces.
format Online
Article
Text
id pubmed-5155295
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-51552952016-12-20 Host cell surfaces induce a Type IV pili-dependent alteration of bacterial swimming Golovkine, Guillaume Lemelle, Laurence Burny, Claire Vaillant, Cedric Palierne, Jean-Francois Place, Christophe Huber, Philippe Sci Rep Article For most pathogenic bacteria, flagellar motility is recognized as a virulence factor. Here, we analysed the swimming behaviour of bacteria close to eukaryotic cellular surfaces, using the major opportunistic pathogen Pseudomonas aeruginosa as a model. We delineated three classes of swimming trajectories on both cellular surfaces and glass that could be differentiated by their speeds and local curvatures, resulting from different levels of hydrodynamic interactions with the surface. Segmentation of the trajectories into linear and curved sections or pause allowed us to precisely describe the corresponding swimming patterns near the two surfaces. We concluded that (i) the trajectory classes were of same nature on cells and glass, however the trajectory distribution was strikingly different between surface types, (ii) on cell monolayers, a larger fraction of bacteria adopted a swimming mode with stronger bacteria-surface interaction mostly dependent upon Type IV pili. Thus, bacteria swim near boundaries with diverse patterns and importantly, Type IV pili differentially influence swimming near cellular and abiotic surfaces. Nature Publishing Group 2016-12-14 /pmc/articles/PMC5155295/ /pubmed/27966607 http://dx.doi.org/10.1038/srep38950 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Golovkine, Guillaume
Lemelle, Laurence
Burny, Claire
Vaillant, Cedric
Palierne, Jean-Francois
Place, Christophe
Huber, Philippe
Host cell surfaces induce a Type IV pili-dependent alteration of bacterial swimming
title Host cell surfaces induce a Type IV pili-dependent alteration of bacterial swimming
title_full Host cell surfaces induce a Type IV pili-dependent alteration of bacterial swimming
title_fullStr Host cell surfaces induce a Type IV pili-dependent alteration of bacterial swimming
title_full_unstemmed Host cell surfaces induce a Type IV pili-dependent alteration of bacterial swimming
title_short Host cell surfaces induce a Type IV pili-dependent alteration of bacterial swimming
title_sort host cell surfaces induce a type iv pili-dependent alteration of bacterial swimming
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155295/
https://www.ncbi.nlm.nih.gov/pubmed/27966607
http://dx.doi.org/10.1038/srep38950
work_keys_str_mv AT golovkineguillaume hostcellsurfacesinduceatypeivpilidependentalterationofbacterialswimming
AT lemellelaurence hostcellsurfacesinduceatypeivpilidependentalterationofbacterialswimming
AT burnyclaire hostcellsurfacesinduceatypeivpilidependentalterationofbacterialswimming
AT vaillantcedric hostcellsurfacesinduceatypeivpilidependentalterationofbacterialswimming
AT paliernejeanfrancois hostcellsurfacesinduceatypeivpilidependentalterationofbacterialswimming
AT placechristophe hostcellsurfacesinduceatypeivpilidependentalterationofbacterialswimming
AT huberphilippe hostcellsurfacesinduceatypeivpilidependentalterationofbacterialswimming