Fractionation of human spermatogenic cells using STA-PUT gravity sedimentation and their miRNA profiling

Human spermatogenic cells have not yet been isolated, and notably, their global miRNA profiles remain unknown. Here we have effectively isolated human spermatogonia, pachytene spermatocytes and round spermatids using STA-PUT velocity sedimentation. RT-PCR, immunocytochemistry and meiosis spread assa...

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Detalles Bibliográficos
Autores principales: Liu, Yun, Niu, Minghui, Yao, Chencheng, Hai, Yanan, Yuan, Qingqing, Liu, Yang, Guo, Ying, Li, Zheng, He, Zuping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155379/
https://www.ncbi.nlm.nih.gov/pubmed/25634318
http://dx.doi.org/10.1038/srep08084

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