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In vitro effectiveness of triterpenoids and their synergistic effect with antibiotics against Staphylococcus aureus strains

OBJECTIVES: The aim of this study is to evaluate the effect of four triterpenoids such as oleanolic acid, ursolic acid, cycloastragenol, and beta-boswellic acid alone and in combination with antibiotics against Staphylococcus aureus strains. MATERIALS AND METHODS: Sixteen clinical strains of S. aure...

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Autores principales: Hamza, Muhammad, Nadir, Maha, Mehmood, Nadir, Farooq, Adeel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155475/
https://www.ncbi.nlm.nih.gov/pubmed/28066112
http://dx.doi.org/10.4103/0253-7613.194851
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author Hamza, Muhammad
Nadir, Maha
Mehmood, Nadir
Farooq, Adeel
author_facet Hamza, Muhammad
Nadir, Maha
Mehmood, Nadir
Farooq, Adeel
author_sort Hamza, Muhammad
collection PubMed
description OBJECTIVES: The aim of this study is to evaluate the effect of four triterpenoids such as oleanolic acid, ursolic acid, cycloastragenol, and beta-boswellic acid alone and in combination with antibiotics against Staphylococcus aureus strains. MATERIALS AND METHODS: Sixteen clinical strains of S. aureus from infected wounds were isolated. Eight were methicillin-sensitive S. aureus (MSSA), and the other eight were methicillin-resistant S. aureus (MRSA). The activity was also seen in reference S. aureus American Type Culture Collection(™) strains. The activity of all the triterpenoids and antibiotics against S. aureus was evaluated by broth microdilution method. The effectiveness was judged by comparing the minimum inhibitory concentrations (MICs) of the compounds with antibiotics. The combination of antibiotics with compounds was evaluated by their fractional inhibitory concentrations (FIC). RESULTS: Against both clinical and reference MSSA strains, none of the compounds exhibited comparable activity to antibiotics vancomycin or cefradine except for ursolic acid (MIC 7.8 μg/ml). Against MRSA, all compounds (MIC 16–128 μg/ml) showed lesser activity than vancomycin (MIC 5.8 μg/ml). Among triterpenoid-antibiotic combinations, the most effective were ursolic acid and vancomycin against clinical strain MSSA (FIC(S) 0.17). However, overall, different combinations between triterpenoids and antibiotics showed 95%–46% (P < 0.05) reduction in MICs of antibiotics compared to when antibiotics were used alone. Cefradine, a drug not suitable for treating MRSA (MIC = 45 μg/ml), showed a remarkable decrease in its MIC (87% P< 0.01) when it was used in combination with oleanolic acid or ursolic acid in both clinical and reference strains. CONCLUSION: The tested triterpenoids are relatively weaker than antibiotics. However, when used in combination with antibiotics, they showed remarkable synergistic effect and thus can help in prolonging the viability of these antibiotics against S. aureus infections. Furthermore, reduction in MIC of cefradine with oleanolic acid indicates their potential use against MRSA.
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spelling pubmed-51554752017-01-06 In vitro effectiveness of triterpenoids and their synergistic effect with antibiotics against Staphylococcus aureus strains Hamza, Muhammad Nadir, Maha Mehmood, Nadir Farooq, Adeel Indian J Pharmacol Short Communication OBJECTIVES: The aim of this study is to evaluate the effect of four triterpenoids such as oleanolic acid, ursolic acid, cycloastragenol, and beta-boswellic acid alone and in combination with antibiotics against Staphylococcus aureus strains. MATERIALS AND METHODS: Sixteen clinical strains of S. aureus from infected wounds were isolated. Eight were methicillin-sensitive S. aureus (MSSA), and the other eight were methicillin-resistant S. aureus (MRSA). The activity was also seen in reference S. aureus American Type Culture Collection(™) strains. The activity of all the triterpenoids and antibiotics against S. aureus was evaluated by broth microdilution method. The effectiveness was judged by comparing the minimum inhibitory concentrations (MICs) of the compounds with antibiotics. The combination of antibiotics with compounds was evaluated by their fractional inhibitory concentrations (FIC). RESULTS: Against both clinical and reference MSSA strains, none of the compounds exhibited comparable activity to antibiotics vancomycin or cefradine except for ursolic acid (MIC 7.8 μg/ml). Against MRSA, all compounds (MIC 16–128 μg/ml) showed lesser activity than vancomycin (MIC 5.8 μg/ml). Among triterpenoid-antibiotic combinations, the most effective were ursolic acid and vancomycin against clinical strain MSSA (FIC(S) 0.17). However, overall, different combinations between triterpenoids and antibiotics showed 95%–46% (P < 0.05) reduction in MICs of antibiotics compared to when antibiotics were used alone. Cefradine, a drug not suitable for treating MRSA (MIC = 45 μg/ml), showed a remarkable decrease in its MIC (87% P< 0.01) when it was used in combination with oleanolic acid or ursolic acid in both clinical and reference strains. CONCLUSION: The tested triterpenoids are relatively weaker than antibiotics. However, when used in combination with antibiotics, they showed remarkable synergistic effect and thus can help in prolonging the viability of these antibiotics against S. aureus infections. Furthermore, reduction in MIC of cefradine with oleanolic acid indicates their potential use against MRSA. Medknow Publications & Media Pvt Ltd 2016 /pmc/articles/PMC5155475/ /pubmed/28066112 http://dx.doi.org/10.4103/0253-7613.194851 Text en Copyright: © Indian Journal of Pharmacology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Short Communication
Hamza, Muhammad
Nadir, Maha
Mehmood, Nadir
Farooq, Adeel
In vitro effectiveness of triterpenoids and their synergistic effect with antibiotics against Staphylococcus aureus strains
title In vitro effectiveness of triterpenoids and their synergistic effect with antibiotics against Staphylococcus aureus strains
title_full In vitro effectiveness of triterpenoids and their synergistic effect with antibiotics against Staphylococcus aureus strains
title_fullStr In vitro effectiveness of triterpenoids and their synergistic effect with antibiotics against Staphylococcus aureus strains
title_full_unstemmed In vitro effectiveness of triterpenoids and their synergistic effect with antibiotics against Staphylococcus aureus strains
title_short In vitro effectiveness of triterpenoids and their synergistic effect with antibiotics against Staphylococcus aureus strains
title_sort in vitro effectiveness of triterpenoids and their synergistic effect with antibiotics against staphylococcus aureus strains
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155475/
https://www.ncbi.nlm.nih.gov/pubmed/28066112
http://dx.doi.org/10.4103/0253-7613.194851
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